ABSTRACT
Breast cancer spreading to different organs have been related to different molecules and mechanisms, but cutaneous metastasis remains unexplored. Increasing evidence showed that MUC1 and some of its carbohydrate associated antigens may be implicated in breast cancer metastasis. In this study we analyzed these tumor markers in order to identify breast cancer cutaneous metastatic profiles. A cohort of 26 primary tumors from breast cancer patients with cutaneous metastases were included; also, cutaneous and lymphatic node metastatic samples and primary tumors from breast cancer patients without metastases were analysed. Immunohistochemical (IHC) studies demonstrated that both underglycosylated MUC1 (uMUC1) and sialyl Lewis x (sLex) to be positively associated with cutaneous metastatic primary tumors (pâ¯<â¯0.05). Notably, a high percentage of tumors with cutaneous metastases were characterized as triple negative and Her2+ tumors (37.5 % and 29 %, respectively). Some discordant results were found between primary tumors and their matched cutaneous metastases. To determine if MUC1 variants may be carriers of carbohydrate antigens, subcellular fractions from a cutaneous metastatic lesion were obtained, immunoprecipitated and analyzed by Western blot. We found that the isolated uMUC1 with a molecular weight of>200â¯kDa was also the site for binding of anti-sLex MAb; in coincidence, a high correlation of positive IHC expression of both markers was observed. Our findings confirm that breast cancer cutaneous metastases were associated to highly malignant primary tumors and sustain the hypothesis that u-MUC1 and sLe x may drive breast cancer cutaneous metastases.
Subject(s)
Biomarkers, Tumor/metabolism , Breast Neoplasms/pathology , Mucin-1/metabolism , Sialyl Lewis X Antigen/metabolism , Skin Neoplasms/secondary , Adult , Aged , Biomarkers, Tumor/analysis , Female , Humans , Middle AgedABSTRACT
El sarcoma del estroma mamario es una variante rara, latamente maligna, de los sarcomas de partes blandas. Su tratamiento convencional es la cirugía, la cual se asocia con un pobre pronóstico. Nosostros reportamos aquí un caso observado en una mujer de 56 años de edad. Una cirugía conservadora fue realizada, seguida de terapia radiante. Sin embargo, se observó una rápida progresión de la enfermedad con desarrollo de metástasis óseas, hepáticas y pulmonares, que resultó refractaria a la terapia sistemática. La sobrevida desde el diagnóstico fue de 16 meses. Una revisión de la literatura acerca del sarcoma del estroma mamario fue conducida (AU)
Subject(s)
Humans , Female , Middle Aged , Breast Neoplasms/pathology , Sarcoma/pathology , Breast Neoplasms/diagnosis , Breast Neoplasms/classification , Sarcoma/diagnosis , Sarcoma/classification , Survival Rate , Prognosis , Mitotic IndexABSTRACT
El sarcoma del estroma mamario es una variante rara, latamente maligna, de los sarcomas de partes blandas. Su tratamiento convencional es la cirugía, la cual se asocia con un pobre pronóstico. Nosostros reportamos aquí un caso observado en una mujer de 56 años de edad. Una cirugía conservadora fue realizada, seguida de terapia radiante. Sin embargo, se observó una rápida progresión de la enfermedad con desarrollo de metástasis óseas, hepáticas y pulmonares, que resultó refractaria a la terapia sistemática. La sobrevida desde el diagnóstico fue de 16 meses. Una revisión de la literatura acerca del sarcoma del estroma mamario fue conducida
Subject(s)
Humans , Female , Middle Aged , Breast Neoplasms , Sarcoma , Breast Neoplasms , Mitotic Index , Prognosis , Sarcoma , Survival RateABSTRACT
Lichen sclerosus (LS) is a chronic inflammatory disease of unknown etiology that may affect the genital and/or extragenital skin of individuals of either sex at all ages. In boys, the prepuce is the most common site of involvement. The diagnostic criteria of LS include the presence of inflammatory infiltrates mainly composed of T lymphocytes. We report on two cases of LS of the prepuce because of the unusual feature of lymphocytic (CD45RO+ and CD20+), histiocytic (CD68+), and granulomatous phlebitis. This lesion was not present in a group of another 18 cases of childhood penile LS. We have not been able to find any references describing and illustrating inflammatory involvement of the dermal vein walls in LS. Unlike the data reported in the literature, the dermal inflammatory infiltrates of these two cases showed a similar proportion of B and T lymphocytes in addition to frequent CD68+ histiocytes.
