ABSTRACT
Se evaluó el efecto toxico del ibuprofeno y meloxicam en la estructura histológica del testículo y calidad seminal de ratas albinas (Rattus norvegicus) variedad Sprague Dawley. Se utilizaron 18 ratas machos de 250 a 300 gramos de peso corporal y de 5 meses de edad, asignados en 3 grupos: grupo 1 control, grupo 2 con una dosis de 120 mg·kg⁻¹·día⁻¹ de ibuprofeno, grupo 3 con una dosis de 1 mg·kg⁻¹·día⁻¹ de meloxicam. Las tabletas de ibuprofeno y meloxicam se diluyeron en agua destilada hasta obtener la concentración previamente establecida, el grupo control recibió solo suero fisiológico. El tratamiento se administró por vía oral usando jeringas de 1 mL provistas de una cánula de intubación oro-esofágica. Concluidos los 30 días, todas las ratas machos fueron eutanizadas con cloroformo comercial y se procedió a extraer los testículos y el epidídimo de cada grupo. El análisis histológico se observó una disminución significativa en la altura de la pared del tubo seminífero así como el diámetro de los mismos; daños severos en las células germinales, congestión vascular intersticial; aumento del tejido conjuntivo, disminución en el número y alteración de la morfología de los espermatozoides en comparación con el grupo control
The toxic effect of ibuprofen and meloxicam on the histological structure of the testes and seminal quality of albino rats (Rattus norvegicus) variety Sprague Dawley was evaluated. Eighteen male rats of 250 to 300 grams body weight and 5 months of age were used, assigned in 3 groups: group 1 control, group 2 with a dose of 120 mg·kg⁻¹·day⁻¹ ibuprofen, group 3 with a dose of 1 mg·kg⁻¹·day⁻¹ meloxicam. Ibuprofen and meloxicam tablets were diluted in distilled water to the previously established concentration, the control group received only physiological serum. Treatment was administered orally using 1 mL syringes provided with a oral-esophageal intubation cannula. At the end of the 30 days, all male rats were euthanized with commercial chloroform and the testes and epididymis were removed from each group. Histological analysis showed a significant decrease in seminiferous tube wall height and diameter; severe damage to germinal cells, interstitial vascular congestion; increased connective tissue, decreased number and morphology of spermatozoa compared to the control group
Subject(s)
Animals , Rats , Ibuprofen/toxicity , Semen Analysis , Testis , Anti-Inflammatory Agents, Non-Steroidal/toxicity , Cyclooxygenase Inhibitors/toxicity , Rats, Sprague-Dawley , Testis/ultrastructure , Case-Control Studies , Teratozoospermia/chemically inducedABSTRACT
1,2,3,4-Tetrahydro-beta-carboline (TH beta C) is an endogenous or environmental neurotoxic factor putatively involved in the development of Parkinson's disease (PD). As part of our efforts to characterize the mechanism of the reported protection of smoking against PD, we have examined the interaction between TH beta C and cigarette smoke. We found that TH beta C reacts in vitro and under physiological conditions with some components of cigarette smoke to form N2-(cyanomethyl)-TH beta C (CM-TH beta C), N2-(gamma-cyanoethyl)-TH beta C (CE-TH beta C), N2-(1'-cyanopropyl)-TH beta C (CP-TH beta C), N2-(1'-cyanobutyl)-TH beta C (CB-TH beta C) and N2-formyl-TH beta C (F-TH beta C). Significant differences in the recovery of some of these TH beta C-derivatives were obtained for Burley and Bright tobacco. Several of the reported compounds showed reversible and competitive MAO-A inhibitory properties. The detection of some of these compounds in rat brain after chronic administration of TH beta C and a solution of cigarette smoke proved that the reported interactions also occur in vivo. These results are discussed as a potential mechanism of neuroprotection in the development of PD.
Subject(s)
Carbolines/pharmacology , Nicotiana , Plants, Toxic , Smoke , Animals , Brain/enzymology , Carbolines/analysis , Carbolines/chemistry , Drug Interactions , Gas Chromatography-Mass Spectrometry , Humans , Male , Monoamine Oxidase Inhibitors/pharmacology , Neuroprotective Agents/pharmacology , Parkinson Disease/prevention & control , Rats , Rats, Sprague-Dawley , Solutions/chemistry , Solutions/pharmacologyABSTRACT
We assessed the potential of 99mTc labelled specific polyclonal antibodies (99mTc-PoAb) for the diagnosis of hydatid disease by immunoscintigraphy. Experimentally infected mice and rabbits were used for this purpose. A specific rabbit antibody recognizing total somatic antigen from hydatid membranes (HCMA) was obtained. PoAb biological activity before labelling was checked according to Barbieri et al. 99mTc-PoAb labelling was performed according to Thakur et al.; the radiochemical purity was higher than 90%. The following studies of 99mTc-PoAb were made: post-labelling biological activity; in vitro stability; blood and renal kinetics in normal mice up to 24 hours after intravenous (i.v.) and intraperitoneal (i.p.) administration; biodistribution in normal and infected mice after i.p. or i.v. injection, and in rabbits after i.v. administration. Biodistribution studies in normal mice, after both administration routes, showed considerable hepatic uptake of activity. An important uptake in cysts after i.p. administration in mice, indicating successful targeting, was also confirmed by autoradiography images. Intravenously administered 99mTc PoAb was not significantly targeted to peritoneal cysts in either animal species, due to inherent limitations to these animal models. Results obtained with i.p. administration suggest that specific hydatid imaging may be possible. Both the mice and rabbit models revealed hepatic uptake which, combined with the short isotope half-life, prevent the drawing of any final conclusions regarding the usefulness of 99mTc-labelling in hydatid disease.
Subject(s)
Antibodies, Helminth , Echinococcosis/diagnostic imaging , Radioimmunodetection , Technetium , Animals , Antibodies, Helminth/blood , Antibodies, Helminth/chemistry , Antibodies, Helminth/metabolism , Autoradiography , Cattle , Disease Models, Animal , Echinococcosis/metabolism , Half-Life , Immunoconjugates/blood , Immunoconjugates/chemistry , Immunoconjugates/pharmacokinetics , Injections, Intraperitoneal , Injections, Intravenous , Kidney/metabolism , Liver/metabolism , Mice , Peritoneal Diseases/diagnostic imaging , Peritoneal Diseases/metabolism , Peritoneal Diseases/parasitology , Rabbits , Technetium/blood , Technetium/chemistry , Technetium/pharmacokinetics , Tissue DistributionABSTRACT
Two cases of subcutaneous infection caused by the primitive aquatic hyphal organism Pythium are described. Pythium is an important pathogen of horses in the U.S.A. and Australia. Cases of human subcutaneous pythiosis have been cited in the literature, but clinical and histopathological features have not been described previously. Both cases occurred in young immunocompetent males in the periorbital region and showed rapid growth, clinically mimicking a tumor and requiring operative biopsy. In both cases there was a history of exposure to either swampy water or horses. The tissue reaction was distinctive, closely resembling that seen in equine pythiosis, comprising well-defined granular eosinophilic islands bordered by macrophages, multinucleate giant cells, fibrosis and numerous eosinophils. Hyphae were well demonstrated with the Grocott stain but only poorly with the PAS method. Identity of the organisms was confirmed with an immunoperoxidase technique employing a polyclonal antiserum to Pythium. Both patients responded well to amphotericin B.
