ABSTRACT
Liquid biopsy has improved significantly over the last decade and is attracting attention as a tool that can complement tissue biopsy to evaluate the genetic landscape of solid tumors. In the present study, we evaluated the usefulness of liquid biopsy in daily oncology practice in different clinical contexts. We studied ctDNA and tissue biopsy to investigate EGFR, KRAS, NRAS, and BRAF mutations from 199 cancer patients between January 2016 and March 2021. The study included 114 male and 85 female patients with a median age of 68 years. A total of 122 cases were lung carcinoma, 53 were colorectal carcinoma, and 24 were melanoma. Liquid biopsy was positive for a potentially druggable driver mutation in 14 lung and colorectal carcinoma where tissue biopsy was not performed, and in two (3%) lung carcinoma patients whose tissue biopsy was negative. Liquid biopsy identified nine (45%) de novo EGFR-T790M mutations during TKI-treatment follow-up in lung carcinoma. BRAF-V600 mutation resurgence was detected in three (12.5%) melanoma patients during follow-up. Our results confirm the value of liquid biopsy in routine clinical oncologic practice for targeted therapy, diagnosis of resistance to treatment, and cancer follow-up.
ABSTRACT
Objective: To compare the health related quality of life (HRQoL) perceptions in parents and physician regarding the patient undergoing ambulatory surgery, and to analyse the evolution of HRQoL before and after the ambulatory surgery. Method: 36 patients underwent ambulatory surgical interventions taken part in this study. The questionnaires EQ-5D-Y were administered to the patients and questionnaires EQ-5D-Y proxy to parents and physicians. Variable TTO was used. Results: Statistical differences were found at baseline between answers of patients and physicians (p < 0.05). However, after surgery, answers of patients and proxies were similar. Conclusions: Paediatric patients undergone to a minor surgery improve HRQoL after the operation and this improvement goes on a month after the surgery.
Objetivo: Comparar la percepción de la calidad de vida relacionada con la salud (CVRS) de padres y médico con respecto al paciente en población infantil sometida a una intervención quirúrgica menor, y analizar la progresión de la CVRS tras un mes de someterse a la intervención. Método: Participaron 36 pacientes sometidos a intervenciones quirúrgicas menores. Se les administró el cuestionario EQ-5D-Y, y su versión proxy correspondiente a los padres y al médico. Se utilizó la variable TTO. Resultados: Los pacientes refirieron un peor estado de salud, sobre todo en la dimensión de preocupación/tristeza, que los médicos en el preoperatorio (p < 0.05), pero la percepción postoperatoria fue similar en pacientes y adultos, atribuible al efecto techo por buena salud postoperatoria. Conclusiones: Los pacientes pediátricos sometidos a cirugía menor mejoran su CVRS tras la intervención, y esta mejora se mantiene tras un mes de la cirugía.
