Fire-Shaped Nozzles to Produce a Stress Peak for Deformability Studies.

Fire-shaped nozzles can be used to study the deformability of microcapsules, particles, or cells traveling in a flow. Though their geometry depends on the dimensions of the original glass capillary and the heating conditions, they all produce a strain rate peak approximately at the section where the diameter is 1.5 times the minimum. The intensity of this peak and the time from its position to the neck can be easily estimated from the flow rate and three geometrical parameters, without the need for any simulation. In the convergent region of these nozzles, it is possible to observe the evolution of the deformation. It is necessary to use a sufficiently long nozzle to produce the maximum deformation before the neck.

Capabilities and Limitations of Fire-Shaping to Produce Glass Nozzles.

Microfluidic devices for drop and emulsion production are often built using fire-shaped (or fire-polished) glass nozzles. These are usually fabricated manually with inexpensive equipment. The shape limitations and poor reproducibility are pointed as the main drawbacks. Here, we evaluate the capabilities of a new fire-shaping approach which fabricates the nozzle by heating a vertical rotating capillary at the Bottom of a Lateral Flame (BLF). We analyze the effect of the heating conditions, and the capillary size and tolerances. The shape reproducibility is excellent for nozzles of the same size produced with the same conditions. However, the size reproducibility is limited and does not seem to be significantly affected by the heating conditions. Specifically, the minimum neck diameter standard deviation is 3%. Different shapes can be obtained by changing the heating position or the capillary dimensions, though, for a given diameter reduction, there is a minimum nozzle length due to the overturning of the surface. The use of thinner (wall or inner diameter) capillaries allows producing much shorter nozzles but hinders the size reproducibility. Finally, we showed an example of how the performance of a microfluidic device is affected by the nozzle shape: a Gas Dynamic Virtual Nozzle (GDVN) built with a higher convergent rate nozzle works over a wider parametric range without whipping.