ABSTRACT
Our goal was to detect whether spotted fever group Rickettsia are found in the suspected vector of rickettsioses, Amblyomma triste, in Uruguay. Rickettsia parkeri was detected in A. triste, which suggests that this species could be considered a pathogenic agent responsible for human rickettsioses in Uruguay.
Subject(s)
Ixodidae/microbiology , Rickettsia Infections/microbiology , Rickettsia/isolation & purification , Rocky Mountain Spotted Fever/microbiology , Tick-Borne Diseases/microbiology , Animals , Female , Humans , Male , Rickettsia/classification , Rickettsia/genetics , Rickettsia Infections/epidemiology , Rocky Mountain Spotted Fever/epidemiology , Tick-Borne Diseases/epidemiology , Uruguay/epidemiologyABSTRACT
As we believe the immunohistochemistry of the hydatid lesions and draining lymph nodes has never been studied, we collected them from the liver and lungs of cattle in Uruguay for such a study. Frozen sections of the tissues were immunohistochemically stained using monoclonal antibodies against surface markers CD2, CD4, CD5, CD8, B cell and granulocyte-monocyte/macrophage and antiserum against specific granules of bovine eosinophils. The adventitial layer of the cyst wall consists of a layer of epithelioid cells and connective tissue. The cells from the epithelioid cell layer were a kind of macrophage. In most cases having progressive hydatid cysts, CD8+ cells were predominant in the pericystic adventitia, and a relatively small number of CD4+ cells were in the same area. In the adventitial layer surrounding the regressive and involutional hydatid cysts, infiltrating lymphocytes were composed mostly of CD4+ cells. An eosinophil-mediated destruction of the laminated layer was recognized in the regressive and involuted hydatid cysts. The subpopulations of T cells in the local lymph nodes tended to be similar to T cells in the adventitial layer of hydatid lesions. From our findings, we consider that infiltration of eosinophils and the subpopulations of lymphocytes infiltrating the hydatid lesions in the liver and lungs are derived from cells in the draining lymph nodes of both organs.
Subject(s)
Cattle Diseases/immunology , Cattle Diseases/pathology , Echinococcosis/veterinary , Lymph Nodes/immunology , Animals , Antigens, CD/analysis , Antigens, CD/classification , Antigens, CD/immunology , B-Lymphocytes/immunology , Cattle , Echinococcosis/immunology , Echinococcosis/pathology , Echinococcosis, Hepatic/immunology , Echinococcosis, Hepatic/pathology , Echinococcosis, Hepatic/veterinary , Echinococcosis, Pulmonary/immunology , Echinococcosis, Pulmonary/pathology , Echinococcosis, Pulmonary/veterinary , Immunity, Cellular , Immunohistochemistry , Lymph Nodes/pathologyABSTRACT
The nematode Lagochilascaris minor was collected from subcutaneous abscesses on the face and neck of three female cats aged 8 months, and 1 and 2 years. These cats were born in the Department of Maldonado, Uruguay. Female and male nematodes were 10.1-14.4 and 10.0-14.3mm long, respectively. The lips were one dorsal lip and two subventral lips separated from the body by a deep post-labial groove, and were separated from each other by triangular interlabial projections. The width of the lips was almost equal to the width of the post-labial collar. The vulva was near the middle of the female body. The eggs were oval or spherical and measured 0.059-0.073 mm long and 0.045-0.065 mm wide. Irregular-shaped pits were on the egg surface; 20-32 pits were around the equator of the eggs. The male posterior region was ventrally curved. Each spicule was about three quarters of the ejaculator duct length. From these findings, the nematodes were identified as L. minor. We believe this is the first report of its infection in Uruguay.
