[Non-gonococcal urethritis and cervicitis. Isolation of Chlamydia trachomatis and detection of anti-Chlamydia antibodies by indirect immunofluorescence]. / Urétrites et cervicites non gonococciques. Isolement de Chlamydia trachomatis et détection des anticorps anti-chlamydiens par immunofluorescence indirecte.

The responsibility of Chlamydia trachomatis in non-gonococcal urethritis and cervicitis was investigated in 267 patients of both sexes. It was confirmed in 36.3% of patients with urethritis and 20.9% of patients with cervicitis by isolating C. trachomatis on Hela 229 cells in the presence of cytochalasin B. No clinical feature specific of C. trachomatis infection could be elicited. The patients were tested for total IgM-type serum anti-chlamydia antibodies by indirect immunofluorescence (IF), using as antigen the inclusions formed in Hela 229 cells by an L2 serotype of C. trachomatis. The serological study was also performed in 86 blood-donors used as controls. The diagnostic value of IF serology is limited in lower genito-urinary infections; the presence of specific IgM's correlates well with the isolation of C. trachomatis, but these IgM's are not detected in protracted urethritis or cervicitis. In such cases, the aetiological diagnosis can only be made by isolation of C. trachomatis from the focus of infection.

[Isolation of Chlamydia trachomatis on Hela 229 cells treated with cytochalasin B in 330 cases of lower genital infections in men and women]. / Isolement de Chlamydia trachomatis sur cellules Hela 229 traitées par la cytochalasine B dans trois cent trente cas d'infections génitales basses de l'homme et de la femme.

A technique of isolating Chlamydia trachomatis on Hela 229 cells treated with cytochalasine B after the inoculation is described. By this technique, Chlamydia trachomatis was isolated from 36,6% of 186 men with non-gonococcal urethritis and from 20,8% of 114 women with signs of cervicitis.

[Distribution of fibronectin in the skin of patients with scleroderma]. / Distribution de la fibronectine dans la peau des sujets sclérodermiques.

The distribution of fibronectin (FN), a major glycoproteic component of extra-cellular matrix, has been studies by an indirect immunofluorescence technique in the skin of 50 normal controls and 19 sclerodermic patients. In the normal skin, FN was present mainly in the papillary dermis, as thin strips and less abundant in reticular dermis, bound to collagen bundles. In scleroderma skins, FN was increased in the deep dermis of extensive and evolutive lesions (11 cases). In an other hand, the distribution of FN was not modified in stabilized lesions (8 cases). We conclude that the detection of FN in the scleroderma skin is an useful marker of the activity of the systemic sclerosis process and we discuss the possible role of FN as a primary matrix for organization of the collagenous connective tissue during the sclerosing process.

[Premalignant fibroepithelial tumors of Pinkus and previous irradiation: report of a survey of 20 patients]. / Tumeurs fibro-épithéliales de Pinkus et antécédents d'irradiations. Analyse d'une étude portant sur 20 sujets.

The search for previous irradiation was done: in nine of the twenty cases radiotherapy or repeated roentgenograms are found, usually twenty years before the start of fibroepithelial tumour. In one case, there was ingestion of arsenic for psoriasis. But in ten cases, no antecedent of irradiation are found (but the interrogatory was sometimes difficult). So it appears that previous irradiation, if it helps starting of fibroepithelial tumour, is not necessary, and the further evolution is the same, with or without irradiation.