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2.
Cell Host Microbe ; 30(4): 518-529.e6, 2022 04 13.
Article in English | MEDLINE | ID: mdl-35247331

ABSTRACT

Phytopathogens like Pseudomonas syringae induce "water soaking" in the apoplastic space of plant leaf tissue as a key virulence mechanism. Water soaking is commonly observed in diverse pathosystems, yet the underlying physiological basis remains largely elusive. Here, we show that one of the strong P. syringae water-soaking inducers, AvrE, alters the regulation of abscisic acid (ABA) to induce ABA signaling, stomatal closure, and, thus, water soaking. AvrE binds and inhibits the function of Arabidopsis type one protein phosphatases (TOPPs), which negatively regulate ABA by suppressing SnRK2s, a key node of the ABA signaling pathway. The topp12537 quintuple mutants display significantly enhanced water soaking after P. syringae inoculation, whereas the loss of the ABA pathway dampens P. syringae-induced water soaking and disease. Our study uncovers the hijacking of ABA signaling and stomatal closure by P. syringae effectors as key mechanisms of disease susceptibility.


Subject(s)
Arabidopsis Proteins , Arabidopsis , Abscisic Acid/metabolism , Abscisic Acid/pharmacology , Arabidopsis/microbiology , Arabidopsis Proteins/genetics , Arabidopsis Proteins/metabolism , Pseudomonas syringae/metabolism , Water/metabolism
3.
Nature ; 592(7852): 105-109, 2021 04.
Article in English | MEDLINE | ID: mdl-33692546

ABSTRACT

The plant immune system is fundamental for plant survival in natural ecosystems and for productivity in crop fields. Substantial evidence supports the prevailing notion that plants possess a two-tiered innate immune system, called pattern-triggered immunity (PTI) and effector-triggered immunity (ETI). PTI is triggered by microbial patterns via cell surface-localized pattern-recognition receptors (PRRs), whereas ETI is activated by pathogen effector proteins via predominantly intracellularly localized receptors called nucleotide-binding, leucine-rich repeat receptors (NLRs)1-4. PTI and ETI are initiated by distinct activation mechanisms and involve different early signalling cascades5,6. Here we show that Arabidopsis PRR and PRR co-receptor mutants-fls2 efr cerk1 and bak1 bkk1 cerk1 triple mutants-are markedly impaired in ETI responses when challenged with incompatible Pseudomonas syrinage bacteria. We further show that the production of reactive oxygen species by the NADPH oxidase RBOHD is a critical early signalling event connecting PRR- and NLR-mediated immunity, and that the receptor-like cytoplasmic kinase BIK1 is necessary for full activation of RBOHD, gene expression and bacterial resistance during ETI. Moreover, NLR signalling rapidly augments the transcript and/or protein levels of key PTI components. Our study supports a revised model in which potentiation of PTI is an indispensable component of ETI during bacterial infection. This revised model conceptually unites two major immune signalling cascades in plants and mechanistically explains some of the long-observed similarities in downstream defence outputs between PTI and ETI.


Subject(s)
Arabidopsis/immunology , NLR Proteins/immunology , Plant Immunity/immunology , Receptors, Pattern Recognition/immunology , Arabidopsis/genetics , Arabidopsis/microbiology , Arabidopsis Proteins/metabolism , NADPH Oxidases/metabolism , Plant Diseases/genetics , Plant Diseases/immunology , Plant Diseases/microbiology , Protein Serine-Threonine Kinases/metabolism , Pseudomonas syringae/immunology , Reactive Oxygen Species/metabolism , Signal Transduction/immunology
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