ABSTRACT
OBJECTIVES: This study aimed to investigate the clinical manifestations, treatment, and prognosis of traumatic pneumolabyrinth caused by tympanic membrane (TM) perforation. METHODS: Clinical data were collected from 3 cases of traumatic pneumolabyrinth occurring between 2015 and 2021 and 22 cases were identified from 20 articles in PubMed database that reported pneumolabyrinth due to tympanum-penetrating injury. INTERVENTION: Nonoperative treatment was performed in Cases 1 and 3. Middle ear inspection was performed 1 year after the injury due to worsening vertigo upon head movement in Case 2. MAIN OUTCOME MEASURES: Hearing outcomes and vestibular evaluations were presented for the 3 cases, and all comparable cases in the literature were reviewed. RESULTS: All 25 patients had a history of traumatic TM perforation, with perforations mostly located in the posterior or posterior superior quadrant (16 cases). Air signs were observed in the vestibule in all 25 patients, 15 of whom revealed stapes luxation into the vestibule. Conservative treatments were performed in 8 cases, and exploratory surgery in 17 cases. Most patients were free of vertigo (23/25). There were no significant hearing improvements in 15 cases, while hearing recovery or improvement was observed in 9 cases. CONCLUSIONS: The clinical manifestations of pneumolabyrinth due to tympanum-penetrating injuries vary widely. Importantly, the degree of hearing loss is not directly related to the subjectively perceived vertigo but to the location and extent of pneumolabyrinth.
ABSTRACT
BACKGROUND: Cancer-associated fibroblasts (CAFs) reconstitute cancer stemness. This study aims to investigate whether the loss of CAF-derived exosomal miR-34c-5p contributes to the maintenance of stem-like properties of laryngeal squamous cell carcinoma (LSCC). METHODS: Exosomes from primarily cultured CAFs and paired normal fibroblasts (NFs) were collected and identified. The differential expression of exosomal miR-34c-5p between CAFs and NFs was detected by next-generation sequencing. In vitro and in vivo assays were performed to examine the effects of miR-34c-5p on the maintenance of stem-like properties. RESULTS: MiR-34c-5p expression is significantly reduced in CAF-derived exosomes. In vitro and in vivo assays revealed that exosomal miR-34c-5p can regulate the stem-like properties of LSCC cells, such as proliferation, invasion, sphere and plate colony formation, chemoresistance, tumorigenicity in nude mice, as well as the expression of cancer stem cell genes. CONCLUSIONS: Loss of miR-34c-5p in CAF-derived exosomes contributes to the maintenance of stem-like phenotypes of LSCC.
Subject(s)
Cancer-Associated Fibroblasts , Laryngeal Neoplasms , MicroRNAs , Animals , Cancer-Associated Fibroblasts/metabolism , Cancer-Associated Fibroblasts/pathology , Cell Line, Tumor , Cell Proliferation/genetics , Gene Expression Regulation, Neoplastic , Humans , Laryngeal Neoplasms/pathology , Mice , Mice, Nude , MicroRNAs/genetics , MicroRNAs/metabolism , PhenotypeABSTRACT
Objective:To investigate the distribution of allergens in patients with allergic rhinitisï¼ARï¼ in Rikaze. Method:A retrospective analysis was performed in patients with AR diagnosed by skin prick test ï¼SPTï¼ between June 2017 and June 2018 from Rikaze People's Hospital. Result:One hundred and thirty-one patients were diagnosed with AR using SPT. The most common inhalant allergens were pollen, including Artemisia sieversiana willdï¼64.8%ï¼, Artemisia annua L. ï¼64.1%ï¼ and Humulus Scandensï¼45.8%ï¼, while soybeanï¼17.6%ï¼, mangoï¼15.3%ï¼ and carpï¼12.2%ï¼ were the most common food allergens. Conclusion:The most common inhalant allergens in patients with AR is the pollen in Rikaze area.
