[Progress in diagnosis and treatment of radioactive iodine-refractory differentiated thyroid carcinoma].

The radioactive iodine-refractory differentiated thyroid carcinoma (RIR-DTC) is a complex process that involves multiple genetic changes and multiple signaling pathways.Radionuclide imaging, genomics and proteomics are effective to clarify the mechanism and helpful in clinical diagnosis and therapy.The treatment of RIR-DTC includes the removal of distant metastases, drug therapy, external radiotherapy and radiofrequency ablation.This review mainly focuses on the pathogenesis, diagnosis and treatment of RIR-DTC.

Comparison of population genetic structures of common wild rice (Oryza rufipogon Griff.), as revealed by analyses of quantitative traits, allozymes, and RFLPs.

We investigated genetic diversity among and within natural populations of Asian common wild rice, Oryza rufipogon, from three different classes of data: quantitative traits, allozymes, and restriction fragment length polymorphisms (RFLPs). The seven populations examined showed polymorphism to varying degrees. The amount of intrapopulation variability appeared to be influenced not only by breeding system but also by the evolutionary history of each population. Interpopulation differentiation was clear, but different classes of data elucidated different patterns. Quantitative traits revealed ecotype differentiation into perennial and annual types of population, whereas allozyme and RFLP analyses revealed geographical differentiation among populations. These results suggest that the diversity patterns shown by quantitative trait analysis reflect mainly the occurrence of adaptive differentiation in response to habitat conditions and that those shown by allozyme and RFLP analyses reflect mainly the effect of isolation by distance. Population differentiation parameters (F(ST)) were highly variable among loci in allozymes as well as in RFLPs.

Mapping QTLs influencing rice floral morphology using recombinant inbred lines derived from a cross between Oryza sativa L. and Oryza rufipogon Griff.

To understand the genetic basis of floral traits associated with the mating system in rice, we analyzed pistil, stamen and glume traits using a recombinant inbred line population, derived from a cross between an Asian cultivated rice ( Oryza sativa L.), Pei-kuh, and a wild rice ( Oryza rufipogon Griff.), W1944. Quantitative trait loci (QTLs) affecting floral morphology were detected by composite interval mapping using a linkage map constructed using 147 markers, mostly RFLPs. A total of 7, 4, 14 and 6 QTLs were detected for traits related to pistil, stamen, and size and shape of the glume, respectively. Comparison of 31 QTLs affecting these organs revealed ten QTLs affecting the different organs in four adjacent regions on chromosomes 2, 4, 5 and 10, but most QTLs (68%) were located separately on the whole chromosomes. Although four QTLs for stigma breadth, anther length and thickness of lemma and palea explained more than 25% of the total phenotypic variance, most QTLs (87%) had smaller effects. These results suggest that quantitative variation observed for pistil, stamen and glume traits is controlled by several distinct genes with small effects.

Identification of AFLP markers closely linked to the rhm gene for resistance to Southern Corn Leaf Blight in maize by using bulked segregant analysis.

Southern Corn Leaf Blight (SCLB) is an important disease in warm-temperate and tropical corn-producing areas throughout the world. We applied a combination of the amplified fragment length polymorphism (AFLP) technique and bulked segregant analysis (BSA) to a large F2 population in order to identify molecular markers linked to the rhm gene for resistance to SCLB. One co-dominant AFLP marker, p7m36, was mapped to a position 1.0 cM from rhm, and we converted this marker to an STS (sequence-tagged site) marker. Combined with the previously identified agrP144, this new marker may be useful for map-based cloning of the rhm gene and marker-assisted selection for rhm.

[Effects of urapidil on cardiovascular response to tracheal intubation and incision during fentanyl co-induction].

To study the effects of urapidil (URA) on cardiovascular response to tracheal intubation and incision during fentanyl co-induction, forty-two patients (ASA I-II) were randomly divided into four groups: Group F1(n = 10), fentanyl 5 micrograms.kg-1 was given by venous injection; Group F2(n = 10), fentanyl 10 micrograms.kg-1; Group U1(n = 10), URA 0.25 mg.kg-1 plus fentanyl 5 micrograms.kg-1; Group U2(n = 12), URA 0.5 mg.kg-1 plus fentanyl 5 micrograms.kg-1. After tracheal intubation, mean arterial pressure (MAP), diastolic pressure (DP) in Group F1 and systolic pressure (SP) in Group U1 were increased more significantly than those in Group U2(P < 0.05). After incision, the values of SP, MAP, DP and rate-pressure product (RPP) in Group F1 were higher apparently than those in Group U2(P < 0.05); at the 5th minute, SP, MAP, heart rate (HR) and RPP in Group F1 were in higher than those in Group F2 (P < 0.05), and SP, MAP in Group U2 were lower than those in Group U1(P < 0.05). It is suggested that the cardiovascular response to tracheal intubation and incision may be effectively inhibited in combination URA with fentanyl, and the dosage of fentanyl might reduce.

[Intraoperative application of acute normovolemic hemodilution].

To investigate the effects of acute normovolemic hemo-dilution(ANHD) on hemodynamics and hemobiochemistry during surgery. In 30 patients(ASA Grade I-III) undergoing selective surgery, ANHD was accomplished by exchanging whole blood(8.0 +/- 2.7) ml.kg-1 with an equal volume of gelofusine after induction of anesthesia. The results showed: after ANHD, Hb, Hct and Plc levels respectively decreased by 13.8%(P < 0.05), 17.1%(P < 0.05) and 7.1%(P > 0.05), and yet kept in normal range; MAP, HR, SpO2, ECG maintained the normal level; and no significant changes were observed in the concentration of Na+, K+ and Cl-. ANHD is a safe and effective technique of autotransfusion, and can decrease or avoid the risk of disease transmission.