ABSTRACT
It was reported that high blood cholesterol levels increased the susceptibility to mitochondrial dysfunction. This study hypothesized that the gestational hypercholesterolemia (HC) could induce the mitochondrial dysfunction in term human placenta. The eligible pregnant women were recruited from Xuanwu Hospital in Beijing during their first prenatal visit (before their 10th week of pregnancy). In total, 19 pregnant women whose serum total cholesterol levels were higher than 7.25 mm at third trimester (measured at 36-38 weeks) were selected as gestational HC. Other 19 pregnant women with normal cholesterol level matched with age, pre-gestational body mass index, and the neonatal gender were included as the control group. Full-term placenta samples were collected. The mitochondrial DNA (mtDNA) copy number, messenger RNA (mRNA) expression of cytochrome c oxidase subunit I, adenosine triphosphate monophosphatase 6 (ATP6ase), citrate synthase, peroxisome proliferator-activated receptor-γ (PPARγ) co-activator 1α, PPARγ co-activator 1ß and estrogen-related receptor-α, and the activity of mitochondrial respiratory chain enzyme complex were measured. Pregnancy outcomes were obtained by extraction from medical records and the labor ward register. The results showed that only placental mtDNA copy number and mRNA expression of ATP6ase were significantly decreased in HC group. No significant differences were detected of other measurements between the two groups. These findings indicated that gestational HC might not induce the damage of placental function seriously.
Subject(s)
DNA, Mitochondrial/genetics , Hypercholesterolemia/physiopathology , Mitochondria/pathology , Mitochondrial Proteins/metabolism , Organelle Biogenesis , Placenta/pathology , Adult , Female , Humans , Mitochondria/genetics , Mitochondrial Proteins/genetics , Placenta/metabolism , PregnancyABSTRACT
Orf is a severe infectious disease of sheep and goats caused by orf virus (ORFV). To investigate the role of ORF119 gene of ORFV, we constructed ORFV with deleted ORF119 gene and LacZ as reporter gene (ORFV-Δ119-LacZ) via homologous recombination. The results showed that wild-type ORF-SHZ1 and ORFV-Δ119-LacZ deletion viruses replicated in Vero cells to similar titers. Relative transcriptional levels of virulence genes OVIFNR, GIF, VEGF and VIL-10 of ORFV-Δ119-LacZ deletion virus were slightly but not significantly lower after 24 hr compared with the wtORF-SHZ1 virus. In vivo experiments showed that 2-month-old lambs inoculated with ORFV-Δ119-LacZ deletion virus exhibited a similar total clinical score compared with those inoculated with wtORF-SHZ1 virus. Based on these results, we conclude that deletion of the ORF119 gene has no significant effect on ORFV replication and virulence.
Subject(s)
Genes, Viral/physiology , Orf virus/physiology , Virus Replication , Base Sequence , Molecular Sequence Data , Orf virus/genetics , Orf virus/pathogenicity , VirulenceABSTRACT
An outbreak of sheep pox was investigated in the Ningxia Hui Autonomous Region in China. Through immunofluorescence testing, isolated viruses, polymerase chain reaction identification, and electron microscopic examination, the isolated strain was identified as a sheep pox virus. The virus was identified through sequence and phylogenetic analysis of the P32 gene, open reading frame (ORF) 095, and ORF 103 genes. This study is the first to use the ORF 095 and ORF 103 genes as candidate genes for the analysis of sheep pox. The results showed that the ORF 095 and ORF 103 genes could be used for the genotyping of the sheep pox virus.
Subject(s)
Capripoxvirus/classification , Capripoxvirus/isolation & purification , Phylogeny , Animals , Base Sequence , Capripoxvirus/ultrastructure , China , Male , Microscopy, Fluorescence , Poxviridae Infections/pathology , Poxviridae Infections/veterinary , Poxviridae Infections/virology , Sheep , Sheep Diseases/virology , Skin/pathology , Skin/virology , Testis/pathology , Testis/virology , Virion/ultrastructureABSTRACT
Two species of Trichinella were identified from China by means of PCR amplification of the mitochondrial small subunit ribosomal DNA and the expansion segment V region of the ribosomal DNA. Seven isolates originating from domestic pig and one isolate originating from dog showed identical DNA banding pattern to Trichinella spiralis, and two isolates from dog showed DNA banding pattern identical to Trichinella nativa. Sequence analysis of the 5S rDNA inter-gene spacer region from the ten Trichinella isolates confirmed the existence of only two Trichinella species and revealed the inner species genetic variation within T. spiralis and T. nativa.
Subject(s)
Dog Diseases/parasitology , Swine Diseases/parasitology , Trichinella/classification , Trichinella/genetics , Trichinellosis/veterinary , Animals , Base Sequence , China/epidemiology , DNA, Helminth/genetics , DNA, Mitochondrial/genetics , DNA, Ribosomal/genetics , Dog Diseases/epidemiology , Dogs , Phylogeny , Polymerase Chain Reaction , Swine , Swine Diseases/epidemiology , Trichinellosis/epidemiology , Trichinellosis/parasitologyABSTRACT
The complete genome of O/Akesu/58 strain of foot-and-mouth disease virus (FMDV) was sequenced. The phylogenetic analysis revealed that it is not closely related to epidemic strains or previous strains compared with reference sequences (the identities of complete VP1 nucleotide sequences range from 77.5 to 84.0%). Its cell-receptor-binding site is a SGD (Ser-Gly-Asp) motif instead of RGD (Arg-Gly-Asp), and 43 bases were deleted in PKs region of the 5'UTR, although deletions were not found in other gene regions.
Subject(s)
Foot-and-Mouth Disease Virus/classification , Foot-and-Mouth Disease Virus/genetics , Genome, Viral , Sequence Analysis, DNA , Animals , Base Sequence , Capsid Proteins/chemistry , Capsid Proteins/genetics , Cattle , China , Molecular Sequence Data , Phylogeny , Sequence Alignment , Species Specificity , Tibet , Viral Proteins/geneticsABSTRACT
China reported the first outbreak of foot-and-mouth disease (FMD) serotype Asia 1 in Chinese Hong Kong in March, 2005. Subsequently, this type of the virus was reported from mainland of China in April 2005. Up to September of 2006, it was detected in more than 15 areas of China. In this paper, the complete genomes of two Chinese isolates, Asia 1/HNK/CHA/05 and Asia 1/JS/CHA/05, of foot-and-mouth disease virus (FMDV) were sequenced and compared with some Chinese sequences and reference sequences from other countries. The identities between Asia 1/HNK/CHA/05 and Asia 1/JS/CHA/05 of 5'-UTR, L gene, P1 (VP1) gene, P2 gene, P3 gene, 3'-UTR are 84.8, 87.6, 86.4 (82.3%), 92.5, 92.8 and 95.3%, respectively. The data revealed that these two strains do not belong to the same genotype depending on the analysis of VP1 sequences, and neither of them have deleted bases in 5'UTR and 3A genes compared with the reference sequences. In addition, the secondary structures of their 5'UTR and 3'UTR are discussed.
Subject(s)
Foot-and-Mouth Disease Virus/classification , Foot-and-Mouth Disease Virus/genetics , Foot-and-Mouth Disease/virology , Genome, Viral , RNA, Viral/chemistry , 3' Untranslated Regions , 5' Untranslated Regions , Amino Acid Sequence , Animals , Asia , Base Sequence , Cattle , China , Molecular Sequence Data , Nucleic Acid Conformation , Phylogeny , RNA, Viral/geneticsABSTRACT
To explore the potential of heterologous protein expression in Kluyveromyces cicerisporus, three expression plasmids, pUK1-PIT, pUKD-PIT and pUKD-S-PIT, based on the vector pUK1 or pUKD were constructed and transformed, respectively, into yeast strain K. cicerisporus Y179U. Human interferon alpha-2a, used as an example protein, was successfully expressed and secreted by transformant Y179U/pUKD-PIT and Y179U/pUKD-S-PIT. In the flask culture, strain Y179U/pUKD-S-PIT could express interferon at 60 mg/l. The stability of plasmid pUKD-S-PIT in the host was higher than that of pUKD-PIT. This was consistent with their expression levels of interferon. There were two interferon-related bands found by Western blotting analysis. The possible reason for this is discussed.
