ABSTRACT
A Gram-stain-negative, aerobic, non-endospore-forming, motile by a polar flagellum, rod-shaped bacterium, designated strain DHOG02T, which produced yellow-pigmented colonies, was isolated from a soil sample collected from the lower subtropical forest of the Dinghushan Biosphere Reserve, Guangdong Province, PR China. Strain DHOG02T grew at 12-37 °C, pH 4-9 and 0-4â% (w/v) NaCl, with optima at 28 °C, pH 6-7 and 0.5â% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that this strain formed a clade with Dyella lipolytica DHOB07T and Dyella jejuensis JP1T, with sequence similarities of 98.0 and 97.4â%, respectively. The result of the concatenated partial gyrB, lepA and recA gene sequence analysis confirmed that strain DHOG02T belongs to the genus Dyella, but is distinct from all currently known species of the genus. The G+C content of the genomic DNA was 62 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminophospholipid and phospholipid. Ubiquinone-8 was the only respiratory quinone detected, and iso-C15â:â0, iso-C17â:â1ω9c and summed feature 3 (C16â:â1ω6c and/or C16â:â1ω7c) were the major fatty acids, all of which supported the affiliation of strain DHOG02T to the genus Dyella. On the basis of the evidence presented here, strain DHOG02T represents a novel species of the genus Dyella, for which the name Dyella halodurans sp. nov. is proposed. The type strain is DHOG02T (=NBRC 111474T=CGMCC 1.15435T).
Subject(s)
Forests , Phylogeny , Soil Microbiology , Xanthomonadaceae/classification , Bacterial Typing Techniques , Base Composition , China , DNA, Bacterial/genetics , Fatty Acids/chemistry , Genes, Bacterial , Nucleic Acid Hybridization , Phospholipids/chemistry , Pigmentation , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Ubiquinone/chemistry , Xanthomonadaceae/genetics , Xanthomonadaceae/isolation & purificationABSTRACT
Two strains, 4G-K17T and 4G-K15, were isolated from forest soil from the Dinghushan Biosphere Reserve, Guangdong Province, PR China (112° 31' E 23° 10' N). The cells of the two strains were Gram-stain-negative, aerobic and non-motile short rods that multiplied by binary division. Strains 4G-K17T and 4G-K15 were obligately acidophilic, mesophilic bacteria capable of growth at pH 3.0-7.0 (optimum 4.0-5.5 and 3.5-5.5, respectively), temperature 12-42 °C (optimum 28 °C) and NaCl concentrations from 0~2.5â% and 0~3.0â% (w/v), respectively. They had a 16S rRNA gene sequence similarity of 99.2â% and showed the highest similarities of 97.1 and 97.4â% to Edaphobacter aggregans Wbg-1T, respectively, which indicated that these two isolates belonged to a novel species of the genus Edaphobacter in subdivision 1 in the family Acidobacteriaceae. The DNA G+C contents of strains 4G-K17T and 4G-K15 were 57.6 and 57.2â%, respectively. They had similar fatty acid profiles, with the major (>10â%) fatty acid profile comprising iso-C15â:â0, C16â:â0 and summed feature 3 (iso-C16â:â1ω7c and/or C16â:â1ω6c), and the major polar lipid profile comprising phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, unidentified aminophospholipids and unidentified phospholipids. All physiological, phylogenetic and chemotaxonomic data suggest that strains 4G-K17T and 4G-K15 belong to a new species of the genus Edaphobacter, for which the name Edaphobacter acidisoli sp. nov. is proposed, with 4G-K17T (=CGMCC 1.15447=LMG 29212) as the type strain.
