ABSTRACT
Experiments carried out on male mice (ICR) demonstrated a protective effect of premedication with the preparation Sho-Saiko-To (TJ 9, Tsumura and Comp.) against the hepatotoxic effects of CCl4 and T1-acetate, which were manifested by increased peroxidation of lipids and increased depletion of reduced glutathion in liver homogenates.
Subject(s)
Chemical and Drug Induced Liver Injury , Drugs, Chinese Herbal/pharmacology , Liver/metabolism , Animals , Carbon Tetrachloride Poisoning/metabolism , Glutathione/metabolism , Lipid Peroxidation , Liver Diseases/metabolism , Male , Mice , Mice, Inbred ICR , Organometallic CompoundsSubject(s)
Glutathione/metabolism , Iron Chelating Agents/pharmacology , Iron/metabolism , Lipid Peroxidation/drug effects , Liver/metabolism , Pyridones/pharmacology , Animals , Cadmium/pharmacology , Carbon Tetrachloride/pharmacology , Deferiprone , In Vitro Techniques , Indium/pharmacology , Liver/drug effects , Male , Malondialdehyde/metabolism , Mice , Mice, Inbred ICRABSTRACT
The influence of several chelating agents (CaDTPA, ZnDTPA, CaEDTA, ZnEDTA, DMSA, D-penicillamine and DMPS, DMP and DDC) on the acute toxicity of CdCl2 and on the whole body retention and tissue distribution of cadmium after the IV application of 115mCdCl2 was compared in mice. The chelating agents were applied immediately after the application of cadmium. CaDTPA, ZnDTPA and DMSA appeared to be the most effective antidotes. However, DMSA increased the amount of cadmium retained in kidneys. The treatment of cadmium-poisoned mice with the combination of DMSA (IP) and ZnDTPA (SC) (all the compounds were injected in equimolar dose) decreased the toxicity of cadmium more than treatment with one chelating agents (given in a 2:1 dose). However, by studying the effect of these chelating agents and their combination of the retention and distribution of Cd in mice, it was demonstrated that the combined application of the antidotes showed little or no improvement over the results obtained with the most effective of the individual components. In the urine of rats injected with CdCl2 and treated with the chelating agents (CaDTPA, ZnDTPA, DMSA), the presence of cadmium complexes was demonstrated. The formation of mixed ligand chelates in vivo was not proved. Experiments in mice given a single injection of 115mCd-labeled Cd complexes of DMPS, DMSA and DTPA showed a high retention of cadmium in the organisms after the IV application of CdDMPS and CdDMSA complexes.
Subject(s)
Cadmium/metabolism , Chelating Agents/pharmacology , Animals , Cadmium/toxicity , Chromatography , Kidney/pathology , Lipid Peroxides/metabolism , Male , Mice , Rats , Tissue DistributionABSTRACT
The effect of the subcutaneous administration of lysine-vasopressin, in doses of 0.625, 1.25, 2.5 and 5 I.U./kg on serum non-esterified fatty acid, triglyceride, cholesterol and glucose levels in fed and fasting female rats was determined. All the doses, in both fed and fasting animals, caused a marked drop in non-esterified fatty acid levels about 15 min after starting the experiment. The only exceptions were fed females given doses 2.5 and 5 I.U./kg, in which non-esterified fatty acid levels rose. Apart from animals given the smallest dose (0.625 I.U./kg), glucose levels rose during the same interval. In the case of triglyceride levels, a dose of 0.625 I.U./kg produced a decrease and the larger doses (1.25, 2.5 and 5 I.U./kg) an increase in fed females. In fasting animals, triglyceride levels fell (statistically non-significantly) after doses of 0.625, 1.25 and 5 I.U./kg, and rose after 2.5 I.U./kg. The cholesterol level showed a temporary tendency to fall in most of the experiments.
Subject(s)
Blood Glucose/metabolism , Cholesterol/blood , Fatty Acids, Nonesterified/blood , Lypressin/pharmacology , Triglycerides/blood , Animals , Dose-Response Relationship, Drug , Fasting , Female , Food , Kinetics , RatsABSTRACT
The effect of subcutaneously injected lysine-vasopressin on serum non-esterified fatty acid, triglyceride, cholesterol and glucose levels in fed and fasting male rats given doses of 2.5 and 5 I.U./kg was determined. About 15 min after starting the experiment, non-esterified fatty acid levels abruptly fell and glucose levels rose in both fed and fasting animals. The only exception were fed males given a dose of 5 I.U./kg in which there was a delayed increase in the non-esterified fatty acid level. Triglyceride levels rose in fed males and fell in fasting males. The cholesterol level fell in both fed and fasting animals.
Subject(s)
Blood Glucose/metabolism , Cholesterol/blood , Fatty Acids, Nonesterified/blood , Lypressin/pharmacology , Triglycerides/blood , Animals , Dose-Response Relationship, Drug , Fasting , Food , Kinetics , Male , RatsABSTRACT
The authors determined the effect of s.c. injected oxytocin on serum non-esterified fatty acid, triglyceride, cholesterol and glucose levels in fed and fasting rats of both sexes, in doses of 2.5 and 5 I.U./kg. No changes in non-esterified fatty acid levels were found in fasting animals of either sex. In fed males, non-esterified fatty acid levels rose abruptly after both doses; in fed females they fell after the larger dose. In fed animals of both sexes, the smaller dose of oxytocin produced a decrease, and the larger dose an increase, in triglyceride levels. In fasting females, triglyceride levels fell after both doses; in fasting males a temporary increase was found after the larger dose. In the great majority of cases cholesterol levels displayed a tendency to fall, in both males and females. In the case of glucose, a transient increase was recorded in fed animals of both sexes after the smaller dose and a decrease in males after the larger dose. In fasting animals, glucose levels temporarily rose in every case.
