Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
Phytochemistry ; 66(7): 811-5, 2005 Apr.
Article in English | MEDLINE | ID: mdl-15797607

ABSTRACT

Six lignans, including the cyclolignan 3,4'-dihydroxy-3',4'-dimethoxy-6,7'-cyclolignan, were isolated from the flowering tops of Larrea tridentata. Additionally the flavanone, (S)-4',5-dihydroxy-7-methoxyflavanone, was isolated for the first time from L. tridentata or any member of the family Zygophyllaceae. All of the compounds were assessed for their growth inhibitory activity against human breast cancer, human colon cancer and human melanoma cell lines. The lignans had IC50 values of 5-60 microM with the linear butane-type lignans being the most potent, and it was found that colon cancer cells were the least sensitive cell type tested. The relative potency of linear butane type lignans against human breast cancer appears to correlate positively with the number of O-methyl groups present on the molecule.


Subject(s)
Antineoplastic Agents, Phytogenic/isolation & purification , Larrea/chemistry , Lignans/isolation & purification , Lignans/pharmacology , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/pharmacology , Breast Neoplasms/drug therapy , Cell Line, Tumor , Colonic Neoplasms/drug therapy , Humans , Inhibitory Concentration 50 , Lignans/chemistry , Melanoma/drug therapy , Molecular Structure
2.
Plant Cell ; 15(8): 1689-703, 2003 Aug.
Article in English | MEDLINE | ID: mdl-12897245

ABSTRACT

We have developed a high-throughput T-DNA insertional mutagenesis program in tomato using activation tagging to identify genes that regulate metabolic pathways. One of the activation-tagged insertion lines (ant1) showed intense purple pigmentation from the very early stage of shoot formation in culture, reflecting activation of the biosynthetic pathway leading to anthocyanin accumulation. The purple coloration resulted from the overexpression of a gene that encodes a MYB transcription factor. Vegetative tissues of ant1 plants displayed intense purple color, and the fruit showed purple spotting on the epidermis and pericarp. The gene-to-trait relationship of ant1 was confirmed by the overexpression of ANT1 in transgenic tomato and in tobacco under the control of a constitutive promoter. Suppression subtractive hybridization and RNA hybridization analysis of the purple tomato plants indicated that the overexpression of ANT1 caused the upregulation of genes that encode proteins in both the early and later steps of anthocyanidin biosynthesis as well as genes involved in the glycosylation and transport of anthocyanins into the vacuole.


Subject(s)
Anthocyanins/biosynthesis , Solanum lycopersicum/genetics , Solanum lycopersicum/metabolism , Amino Acid Sequence , Anthocyanins/chemistry , Anthocyanins/metabolism , Base Sequence , DNA, Bacterial/genetics , DNA, Plant/genetics , Genes, Plant , Solanum lycopersicum/anatomy & histology , Molecular Sequence Data , Mutagenesis, Insertional , Phenotype , Pigmentation/genetics , Pigmentation/physiology , Plant Proteins/genetics , Plants, Genetically Modified , Sequence Homology, Amino Acid , Transcriptional Activation
SELECTION OF CITATIONS
SEARCH DETAIL
...