Susceptibility to measles among health workers in a university hospital in southern Italy.

OBJECTIVES: Measles still has a high impact on the health of the population in Italy and therefore requires a strong commitment to prevention at national level. In addition to Italy, measles outbreaks have also been reported in other EU countries, with a high number of cases and a rapid spread of the disease even in the nosocomial context between patients and health personnel. The aim of this study is to evaluate the prevalence of measles in a group of health workers working at a university hospital in southern Italy. MATERIALS AND METHODS: A seroepidemiological study was conducted on 458 health workers. Measles antibody IgG and IgM levels were evaluated by immunoenzymatic testing. RESULTS: The highest percentage of susceptible subjects was ≤30 years old, with a statistically significant difference compared to the age group ≥51 years. With regard to gender, susceptibility to measles in males was significantly higher than in females (p<0.05). Additional statistically significant differences were found in the different age groups in both genders. CONCLUSIONS: Although the results show that most health workers are immune to measles, a 20% susceptibility certainly represents a risk for the spread of the disease among operators and patients. Vaccination and control of suspected cases, especially in community settings such as the hospital environment, are the main measures to prevent the transmission and spread of the disease.

Mediterranean diet: knowledge and adherence in Italian young people.

INTRODUCTION: In November 2010 the Mediterranean Diet was recognized by the UNESCO as Intangible Cultural Heritage of Humanity. It is an heritage that brings together the dietary habits of the peoples of the Mediterranean countries, consolidated over the centuries remained almost unchanged until the 1950s. Numerous scientific studies have also shown that the Mediterranean Diet is an healthy diet that helps to prevent the main chronic diseases such as cardiovascular diseases, diabetes, bulimia and obesity and thanks to the antioxidant power of olive oil combined with vegetables consumption, an important means of cancer prevention. The aim of our study was to investigate the knowledge and the adherence to the Mediterranean diet of young people living in the Mediterranean area. MATERIALS AND METHODS: The survey was carried out by administering a web-based anonymous questionnaire from March to May 2019 to Italian young people. In this survey we asked for socio-economic conditions, knowledge, awareness and adherence to the Mediterranean diet (through the KIDMED test) both in childhood and at present time. Parental educational status and family income were used as indicators of socio-economic status (SES). RESULT: The sample was made up by 507 Italian young people between the ages of 17 and 35 (123 males and 384 females), with an average age of 22.88 years (DS 7.02). Only 11.4% knew the Mediterranean diet. During childhood 70.8% of respondents had breakfast and 58% ate fruit and vegetables daily. Today they have maintained the correct eating habits, in fact, 71.2% had breakfast, 60.2% ate fruit and 64.7% ate vegetables. In addition, only 48.3% eat fish and the majority of respondents consume carbohydrates (76.9%). To a lesser extent they consume junk foods like candy and various snacks (18.1%) or fast food meals (9.1%). Moreover, we asked for physical activity and we found that 48.9% did it. We found age, exercise and sex high significant variables (p<0.01). Higher adherence to MD was found in older interviewees. CONCLUSION: Our study we found high knowledge to a Mediterranean diet into 90.5% of the sample and poor adherence into 23.5% of them, medium into 53.4% while higher only in 23.1% of the interviewees. Lower adherence was found among those who do not engage in physical activity and the two regressor with higher weight on the adherence to MD were "eat at home "and "eat fruit and vegetables". Furthermore, in our sample more increased the age more increased adherence to traditional diet models.

Seroprevalence of hev antibodies in a sample of pregnant women in the city of Messina.

BACKGROUND: Hepatitis E virus (HEV) is widespread in developing countries and the disease is also increasing in the developed ones. This infection in pregnancy can cause spontaneous abortion and neonatal death in 56% of newborns. STUDY DESIGN: The study was conducted on a sample of 352 pregnant women, 326 Italian and 26 foreign, in order to confirm the presence of HEV in our territory, to analyze wrong habits of the population and to suggest preventive actions against the risk to contract the infection during pregnancy. METHODS: We asked all women under study to fill an anonymous questionnaire immediately before taking a venous blood samples to determine the presence of anti-HEV antibodies. The questionnaire contained a set of questions to gain information about lifestyles and risk factors to contract HEV. RESULTS: The questionnaire revealed that a portion of the tested women have risk behaviours, as consumption of raw or undercooked food, eating unwashed food and traveling to endemic areas. The percentage of women positive for HEV antibodies was 3.4%, in agreement with national data; all the women were Italian. CONCLUSIONS: This study confirms the circulation of HEV in the city of Messina. For this reason, it is highly recommended to disseminate hygienic and appropriate behaviours and feeding habits in order to prevent the risk to contract the infection.

Response to anti-HBV vaccine and 10-year follow-up of antibody levels in healthcare workers.

BACKGROUND: Despite improvements in public health and antiviral treatments, vaccination is still the most effective means of prevention of hepatitis B virus (HBV) infection. However, little is known about the duration of protection given by the anti-HBV vaccine. Healthcare workers represent a category at risk not only of contracting infection but also of being a source of contagion to patients. OBJECTIVES: To assess individual responses to the anti-HBV vaccine and duration of protection 10 years after its administration in a cohort of healthcare workers employed by the University Hospital 'G. Martino' in Messina, Italy. METHODS: One hundred and seventy medical staff who had been vaccinated following an incident carrying risk of HBV infection were included in this study. The group was followed over a 10-year period, and HBV antibody levels were assessed using an automated microparticle enzyme immunoassay. RESULTS: Protective antibody levels (≥10 mIU/ml) were found in 65% of subjects who had completed the full vaccine schedule (three doses) and in 35% of subjects who had only received one or two doses of anti-HBV vaccine. Moreover, 10 years after vaccination, HBV antibody levels were inversely related to age at vaccination (P < 0.001). No differences were found between males and females. CONCLUSIONS: This study, in line with the literature, shows the importance of completing the full vaccine schedule (three doses). Moreover, in order to have an effective and durable antibody response and avoid the risk of contracting HBV after an injury at work, it is important to recommend anti-HBV vaccination at a young age, ideally during childhood in accordance with the national vaccination policy.

Hepatitis E virus (HEV) in sewage from treatment plants of Messina University Hospital and of Messina City Council.

Samples of sewage from treatment plants at the "G. Martino" University Hospital of Messina (AOU) and that of Messina City Council were analysed to detect the hepatits E virus. Samples were taken on sewage entering and exiting the treatment plants on a monthly basis over a one-year period from both the hospital plant (24 samples) and the municipal plant (22 samples). All sewage samples were pretreated by ultrafiltraton and concentration and finally processed by the PCR method to amplify gene material. A total of three samples tested positive: two (8.33%) entering the AOU treatment plant and one (4.5%) entering the municipal plant while no cases of HEV were detected in samples of treated sewage. These findings confirm the presence of the virus in the city of Messina and showed that the two treatment plants to be working efficiently when tested.

Eliminating congenital rubella: a seroepidemiological study on women of childbearing age and MMR vaccine coverage in newborns.

INTRODUCTION: Rubella can have particularly serious effects on the product of conception if contracted during pregnancy. Thus, the main aim of rubella vaccination programmes is to prevent infection during pregnancy. MATERIALS AND METHODS: A seroepidemiological study was conducted from July 2006 to December 2007 on 1000 women of childbearing age, 15 to 45 years old, using specific rubivirus antibody assays, IgG and IgM. A questionnaire administered at the same time allowed us to survey how much women knew about this disease. In addition, MMR vaccine coverage rates were analysed for cohorts born in the local health districts of Messina for the period 1993-2006. RESULTS: An analysis of the replies given to the questionnaire showed an estimated 42.8% of the women to have immunity from rubella, while the serological study showed an immunity coverage rate of80.6%. Vaccination coverage in the local health districts regarding the first dose of MMR was 81% (cohorts 1993-2005), while the rate was only 24% for the second dose (cohorts 1993-2002). CONCLUSIONS: Both immunity coverage in women of childbearing age and that for newborns (for the cohort considered)fall below the 95% target set by the National Elimination Plan for Measles and Congenital Rubella (PNEM). It is therefore necessary to provide women with adequate information about the risks of rubella during pregnancy and about the benefits of vaccination, as well as to recoup subjects at risk or those whose immune status is unknown. Public health authorities also need to make continued efforts to increase the number of MMR vaccinations throughout the region.

Direct viable count as test for toxicity assessment: the effects of four metals on a Salmonella enteritidis strain.

The toxicity of synthetic sewage containing increasing concentrations of arsenic (.125, .25, .5, 1.0 mg L-1), cadmium (.02, .05, .1, .2 mg L-1), lead (.2, .5, 1.0, 2.0 mg L-1) and nickel (.5, 1.0, 2.0, 4.0 mg L-1) has been investigated by determining the total direct count (TDC) and the direct viable count (DVC) of Salmonella enteritidis by means of an immunofluorescence technique (IFA). This has been done in order to evaluate the possibility of using the IFA technique to estimate the toxicity of complex effluents. Arsenic, cadmium and nickel produced a concentration-dependent reduction in the number of viable bacterial cells. This was more clear when the viable bacterial cells were considered than when only the culturable part was used. Lead did not show a concentration-dependent and reproducible effect. At the highest concentrations allowed by the Italian wastewater regulations, lead, cadmium, arsenic and nickel reduced the viable/total bacterial cells ratio to 74.5%, 68.5%, 28.4% and 6.9%, respectively. The toxic effects of the metals were also tested using the standard Microtox assay.

Effect of metal mixtures on Salmonella enteritidis viability in synthetic sewage.

We sought to determine the effect of 26 multiple metals solutions, containing Cd, Pb, As and/or Ni, on the viability of Salmonella enteritidis. We used the direct viable count effected by immunofluorescence technique (IFA-DVC) in comparison with the culturable cell count. The metals concentrations were prepared considering the single as well as the overall limits provided by Italian regulation on the water pollution. The mixtures containing nickel showed always toxicity more elevated than other metal solutions tested. Thus we consider too high the nickel law limit. The interactions among the elements, causing generally toxicity decrease, are very well assessable by IFA-DVC test. This method permits to calculate the lethal concentrations of toxicants on bacteria that play a pivotal role on the ecosystem components.

The presence of anti-Borrelia burgdorferi antibodies in a group of multiple sclerosis patients in eastern Sicily. Preliminary data.

The authors evaluate the presence of anti-Borrelia burgdorferi antibodies in a group of polysclerotic patients of Eastern Sicily, in order to verify or dismiss a correlation between Borrelia infection and demyelinizing syndrome. 460 samples of serum were examined, of which 60 belonged to patients affected by definite multiple sclerosis; the other 400 were taken from a group of apparently healthy subjects. From the analysis of the data obtained it can be seen that of the 60 polysclerotic subjects examined, 12 equal to 20% were found to be positive for the presence of IgG anti-Borrelia burgdorferi, while in the control group 30 subjects were found to be positive, equal 7.5%.