Occupational health and safety, metal exposures and multi-exposures health risk in Canadian electronic waste recycling facilities.

Electronic waste recycling (e-recycling) involves manual operations that expose workers to toxic metals. We aim to describe occupational health and safety practices and workers' exposures to metals in the Canadian formal e-recycling industry, and to estimate the health risk associated with multiple exposures. This cross-sectional study documented practices through observations and questionnaires, and assessed metal exposures using personal air samples and biomarkers. Health risks were estimated relative to recognised occupational exposure guidelines, and using an additive approach for consideration of multiple exposures. Six e-recycling and one commercial recycling facilities were investigated, and the metal exposures of 99 workers (23 women) were measured. In most facilities, dust control was inadequate and personal protective equipment was improperly worn. In e-recycling, lead was detected in all air samples and in most blood samples, up to 48 µg/m3 and 136 µg/l, respectively. Other quantified metals included beryllium, mercury, arsenic, barium, cadmium, chrome, cobalt, copper, indium, manganese, nickel and yttrium. When handling cathode ray tube screens, workers were 4.9 times and 8.5 times more likely to be exposed to lead and yttrium, respectively, than workers who were not assigned to a specific type of electronics. Overall, exposures were largely associated with facility size and airborne dust concentration. The additive hazard indices for airborne exposures raised concerns for kidney disorders, for peripheral and central nervous systems, and for the male reproductive system. Minimizing airborne dust through collective control methods and adequately using personal protection should reduce metal exposures and associated health risks in this growing industry.

Acyclic tethers mimicking subunits of polysaccharide ligands: selectin antagonists.

We report on the design and synthesis of molecules having E- and P-selectins blocking activity both in vitro and in vivo. The GlcNAc component of the selectin ligand sialyl Lewis(X) was replaced by an acyclic tether that links two saccharide units. The minimization of intramolecular dipole-dipole interactions and the gauche effect would be at the origin of the conformational bias imposed by this acyclic tether. The stereoselective synthesis of these molecules, their biochemical and biological evaluations using surface plasmon resonance spectroscopy (SPR), and in vivo assays are described. Because the structure of our analogues differs from the most potent E-selectin antagonists reported, our acyclic analogues offer new opportunities for chemical diversity.

A new approach to explore the binding space of polysaccharide-based ligands: selectin antagonists.

The discovery of molecules that interfere with the binding of a ligand to a receptor remains a topic of great interest in medicinal chemistry. Herein, we report that a monosaccharide unit of a polysaccharide ligand can be replaced advantageously by a conformationally locked acyclic molecular entity. A cyclic component of the selectin ligand Sialyl Lewis(x), GlcNAc, is replaced by an acyclic tether, tartaric esters, which link two saccharide units. The conformational bias of this acyclic tether originates from the minimization of intramolecular dipole-dipole interaction and the gauche effect. The evaluation of the binding of these derivatives to P-selectin was measured by surface plasmon resonance spectroscopy. The results obtained in our pilot study suggest that the discovery of tunable tethers could facilitate the exploration of the carbohydrate recognition domain of various receptors.