Evaluation of a modified atmosphere packaging system in pallets to extend the shelf-life of the stored tomato at cooling temperature.

A modified atmosphere packaging (MAP) system in pallets was developed and its effect on physico-chemical and sensory characteristics and shelf-life of tomato was evaluated. Tomatoes were stored at 6 °C in cardboard boxes arranged on pallets wrapped in micro-perforated low-density polyethylene (LDPE) bags. Effects of the storage time and packaging were evaluated after 0, 7, 14, and 21 days of storage. The MAP system with pallets assessed, using a packaging atmosphere composition of 10% O2 - 10% CO2 and silica gel as an adsorbent, extended the shelf-life of the tomato stored at refrigeration temperature. MAP delayed color evolution and reduced the firmness loss, biosynthesis of lycopene, and decay rate of tomato. At the end of storage, 100% of the unpackaged samples showed spots while only 42.9% of MAP samples had them. In addition, the percentage of tomatoes with cracks and stretch marks was reduced from 42.9% (unpackaged tomatoes) to 14.3% (MAP tomatoes).

Validation of a Histologic Scoring Index for C3 Glomerulopathy.

RATIONALE & OBJECTIVE: A previous study that evaluated associations of kidney biopsy findings with disease progression in patients with C3 glomerulopathy (C3G) proposed a prognostic histologic index (C3G-HI) that has not yet been validated. Our objective was to validate the performance of the C3G-HI in a new patient population. STUDY DESIGN: Multicenter, retrospective cohort study. SETTING & PARTICIPANTS: 111 patients fulfilling diagnostic criteria of C3G between January 1995 and December 2019, from 33 nephrology departments belonging to the Spanish Group for the Study of Glomerular Diseases (GLOSEN). PREDICTORS: Demographic, clinical parameters, C3G-HI total activity score, and the C3G-HI total chronicity score. OUTCOME: Time to kidney failure. ANALYTICAL APPROACH: Intraclass correlation coefficients and κ statistic were used to summarize inter-rater reproducibility for assessment of histopathology in kidney biopsies. The nonlinear relationships of risk of kidney failure with the total activity score and total chronicity score were modeled using Cox proportional hazards analysis that incorporated cubic splines. RESULTS: The study group included 93 patients with C3 glomerulonephritis and 18 with dense-deposit disease. Participants had an overall meanage of 35±22 (SD) years. Forty-eight patients (43%) developed kidney failure after a mean follow-up of 65±27 months. The overall inter-rater reproducibility was very good for the total activity score (intraclass correlation coefficient [ICC]=0.63) and excellent for total chronicity score (ICC=0.89). Baseline estimated glomerular filtration rate (eGFR), 24-hour proteinuria, and treatment with immunosuppression were the main determinants of kidney failure in a model with only clinical variables. Only tubular atrophy and interstitial fibrosis were identified as predictors in a model with histological variables. When the total activity score and total chronicity score were added to the model, only the latter was identified as an independent predictor of kidney failure. LIMITATIONS: Only a subset of the kidney biopsies was centrally reviewed. Residual confounding. CONCLUSIONS: We validated the performance of C3G-HI as a predictor of kidney failure in patients with C3G. The total chronicity score was the principal histologic correlate of kidney failure.

Effect of high-pressure processing pretreatment on the physical properties and colour assessment of frozen European hake (Merluccius merluccius) during long term storage.

Fish freshness is lost by autolytic degradation produced by endogenous enzymes. Frozen storage is one of the most used methods to preserve fish properties. However, protein denaturation has shown to be a major problem for frozen European hake (Merluccius merluccius), leading to texture losses and off-odour development. The aim of this work was to study the changes produced by high-pressure processing (HPP) before freezing on quality of frozen European hake stored at -21 °C for 12 months. The effect of HPP (150-450 MPa) on mechanical properties and expresible water was evaluated in raw and cooked fish samples. The effect on colour (L*, a* and b*) was assessed only in raw fish. Results showed that HPP before freezing is beneficial to maintain expresible water in good levels up to 6 months. The luminosity significantly increased with pressure level. Textural profile of raw samples showed that HPP increased hardness, adhesiveness and springiness of frozen hake. Cooked samples were also affected by HPP, being the best results obtained at 300 MPa for 6 months of frozen storage. Overall, results showed that HPP improves the quality of frozen hake.

Honeydew Honeys: A Review on the Characterization and Authentication of Botanical and Geographical Origins.

The commercial interest in honeydew honeys (from the secretions of plants or the excretions of plant-sucking insects found on plants) is increasing because of their higher therapeutic properties compared with those of most blossom honeys (from nectar). However, honeydew honeys have been less studied than blossom honeys. In this work, studies carried out to characterize and authenticate honeydew honeys by their botanical and geographical origins have been reviewed. The identification of honey origins has been approached by two ways: by the analysis of chemical markers and by the development of analytical methodologies combined with multivariate analyses. Some compounds have been suggested as specific botanical markers of several honeydew honeys, such as quercitol and trans-oak lactone for oak honey, 2-aminoacetophenone and propylanisol for holm oak honey, and 1-chloro-octane and tridecane for pine honey. The presence of 3-carene and an unidentified compound in samples was proposed as a way discriminate between Greek and Turkish pine honeys. Chemometric analyses have been applied on chemical compositions and on physicochemical, microscopic, and spectral parameters and have proved to be valuable methods for authenticating honeydew honeys. Analytical methods based on spectral information are suitable for the routine control of honeydew-honey origins because they are fast and require easy sample preparations.

Analytical Methods Used in the Quality Control of Honey.

Honey is a natural sweet substance produced by bees (Apis mellifera). In this work, the main parameters used in routine quality control of honey and the most commonly used analytical methods for their determination are reviewed. Honey can be adulterated with cheaper sweeteners or, indirectly, by feeding the bees with sugars. Therefore, methods for detecting and quantifying adulteration are necessary. Chromatographic techniques are widely used in honey analysis. More recently, techniques such as Raman, near-infrared, mid-infrared, and nuclear magnetic resonance spectroscopy in combination with chemometric data processing have been proposed. However, spectroscopy does not allow the determination of enzyme activities, one criteria of great importance for the honey trade. Methylglyoxal is an interesting compound for its antibacterial properties. Methods for its determination are also reviewed.

Short- and medium-term increase of CA125 in peritoneal effluent using a neutral-pH solution.

OBJECTIVE: To investigate the effects of an alternative peritoneal dialysis (PD) solution composed of a lactate/ bicarbonate (Lac/Bic) mixture (35/2 mmol/L), pH 7.0, on the appearance of cancer antigen 125 (CA125) in the peritoneal effluent. METHODS: Eight stable PD patients received a conventional solution containing 35 mmol/L lactate (Lac) for 11.9 +/- 9.2 months, and then changed to Lac/Bic for 3.1 +/- 0.7 months. Each patient acted as his/her own control. RESULTS: We studied 4 males and 4 females with a mean age of 57.4 +/- 16.8 years. Higher concentrations (U/mL) (p < 0.005) and appearance rates (AR) (U/min) (p < 0.05) of CA125 in the dialysate were observed with Lac/Bic than with Lac, during the sampling times of the peritoneal equilibration test (10, 120, and 240 minutes) and in the overnight effluent: CA125 5.7 versus 0.6, 18.9 versus 1.9, 29.7 versus 3.7, and 43.2 versus 5.5 U/mL; and AR 1177 versus 125,354 versus 36,297 versus 37, and 194 versus 26 U/min, respectively. Mean CA125 content in the 24-hour dialysate was 34.2 U/min (baseline) and 30.9 U/min (11.9 months) with Lac, and 207.9 U/min and 185 U/min after 1.6 and 3.1 months with Lac/Bic (p = 0.009). The intraperitoneal pH was more physiological during the dwell with the Lac/Bic solution. CONCLUSIONS: The CA125 levels in the peritoneal effluent with Lac/Bic are an effect of the solution's neutral pH, as other factors of the prescription were constant. The Lac/ Bic solution is more biocompatible than Lac, reflecting short- and medium-term changes in the mesothelial cells, whose clinical significance has not yet been determined.