Effect of androgenic anabolic steroids on sperm quality and serum hormone levels in adult male bodybuilders.

The purpose of this study was to assess the influence of the administration of high doses of androgenic anabolic steroids (AAS) on endocrine and semen parameters. Thirty volunteering bodybuilders were studied (ages ranging between 26.6 +/- 4.1 years). A history of anabolic steroid administration was recorded for fifteen subjects, and results of semen analysis and endocrine parameters were compared with data from fifteen bodybuilders not using steroids. In those subjects using AAS, eight had sperm counts under the lower normal limit (20 x 10(6) sperm/ml), three had azoospermia, two polyzoospermia, and two had normal sperm counts. The percentage of morphologically normal sperm was significantly reduced, only 17.7% had normal spermatozoa. In the control group, only one subject had oligozoospermia. The hormonal parameters revealed reduced FSH (1.5 +/- 3.2 vs 5.0 +/- 1.6, p < 0.001) and PRL (5.1 +/- 4.9 vs 9.2 +/- 4.4, p < 0.01) levels. LH, T, E2 and DHEA levels did not vary.

[Estradiol receptors in cervical intraepithelial neoplasia and cervico-uterine cancer]. / Receptores a estradiol en neoplasia intraepitelial cervical y cáncer cervicouterino.

The objective of this study was to know if there is a correlation in contents o (R + E) in biopsies from patients pre and post-menopausal, in normal cervix with cervical intraepithelial neoplasia (NIC) and invasive cancer. Thirty four patients with abnormal cytology; colposcopy was carried out, and two biopsies were taken from the suspicious lesion, they were sent for histopathological study, and for estrogenic receptors; both studies were correlated later. It was seen that (R +/- E) concentration is higher in intraepithelial lesions of low and high degree than in control patients; and it was not so in premenopausal with invasive cancer where (R +/- E) are much lower, as to postmenopausal (R + E) concentration is higher specially in invasive cancer. It is concluded that the study on quantification to (R + E) in cervical pathology means a good alternative in hormonal treatment, as it has been seen in mammary pathology, and so, more research has to be done.

[Infiltrating duct breast carcinoma: the role of estradiol and progesterone receptors]. / Carcinoma mamario ductal infiltrante. Papel de los receptores a estradiol y progesterona.

The content of receptors to estradiol (ER) and progesterone (PgR) was studied in hormone-dependent tumours from pre- and postmenopausal patients with an infiltrating ductal type of mammary cancer in clinical stage III. The receptors concentration was correlated with the response endocrine treatment. (ER) and (PgR) demonstrated values in the ranges 11-275 and 11-76 femtomoles/mg protein respectively. According to the type of receptor found, four different groups were found, the group (ER+) (PgR+) was found in 25% and 26% of the pre- and postmenopausal patients respectively, 31% and 27% demonstrated the group (ER+) (PgR-). The combination (ER-) (PgR+) was present in 21% of premenopausal women and 16% in the postmenopausal conditions. 23% and 31 of the pre- and postmenopausal women presented (ER-) (PgR-) respectively. Patients, to whom tamoxifen was administered, were those who demonstrated the following receptor groups: (ER+) (PgR+), (ER+) (PgR-) and (ER-) (PgR+). Following therapeutic treatment those surviving without tumoural activity, were those who demonstrated higher estrogen receptor values. The concentration of (ER) was observed to be decreased by 29% in patients who presented distant metastasis and in the cases with local metastasis, (ER) showed at 54% decrease with respect to (ER) values from symptomatic patients. The concentration of (PgR) remained constant in all the aforementioned groups.

[Estradiol, progesterone, and dexamethasone receptors in infiltrating ductal carcinoma]. / Receptores a estradiol, progesterona y dexametazona en carcinoma ductal infiltrante.

The concentration of receptor to estradiol (E-R) progesterone (Pg-R) and dexamethasone (G-R) was studied, by the dextran coated charcoal method in the cytosol of the mammary gland tissue from patients with infiltrating ductal carcinoma. The study was done in one hundred cases. 45% of these patients were postmenopausic and 55% premenopausic, with average ages of 66 and 37 years respectively. Their first pregnancy occurred on average at 24 years and they had 3-4 gestations, 45% breast-fed for a minimum period of six months and menarche at 13 years. The specific union found in the tumours was considered "receptor positive" were in the range of 10 to 181 femtomoles/mg. The "Receptor negative" tumour was considered to have a specific union less than 10 femtomoles/mg protein. On using E-R as a marker to predict the hormonal treatment it was found in this study that 56% of these patients demonstrated R-E+ and was absent in 44%. Simultaneous determination of E-R and Pg-R, raised the number of patients presenting specific receptors to 70% and on determining E-R, Pg-R and G-R, in the same tumours as markers of hormone dependence, the number of patients that presented them, increased to 77%. Finally 23% of these patients did not contain E-R, Pg-R or G-R. When the values of R-E+ were grouped according to the menstrual state of the patient, it was found that the high values of R-E+ corresponded to the postmenopausic patients. No relation was found between the concentration of R-Pg+ and R-G+ and the patient's menstrual state.