ABSTRACT
Mycobacterium abscessus complex (MABSC) subspecies differentiation improves patients' therapy and outcome. Fourier-Transform-Infrared Spectroscopy (FT-IRS) was applied for subspecies discrimination of 15 strains on different media: Löwenstein-Jensen showed the best resolution power; Linear Discriminant Analysis model differentiated M. abscessus susbsp. abscessus from M. abscessus subsp. massiliense. FT-IRS has a potential role in rapidly MABSC subspecies identification.
Subject(s)
Mycobacterium abscessus , Humans , Spectroscopy, Fourier Transform InfraredSubject(s)
Sporotrichosis/diagnosis , Tuberculosis, Cutaneous/diagnosis , Ulcer/pathology , Humans , Male , Middle Aged , Skin/pathologyABSTRACT
This report describes a case of Sphingobacterium hotanense bacteraemia in a patient scratched by a rooster on the right arm. Diagnostic, clinical and therapeutic features are discussed. To the best of our knowledge, this is the first case of Sphingobacterium hotanense bacteremia reported in the medical literature.
Subject(s)
Bacteremia/diagnosis , Bacteremia/microbiology , Soil Microbiology , Sphingobacterium/isolation & purification , Sphingobacterium/pathogenicity , Aged, 80 and over , Amoxicillin/therapeutic use , Animals , Anti-Bacterial Agents/therapeutic use , Atrioventricular Block/therapy , Bacteremia/drug therapy , Chickens , Clavulanic Acid/therapeutic use , Drug Therapy, Combination , Humans , Male , Pacemaker, Artificial , Risk Factors , Sphingobacterium/classification , Treatment Outcome , beta-Lactamase Inhibitors/therapeutic useABSTRACT
Six strains of a rapidly growing scotochromogenic mycobacterium were isolated from pulmonary specimens of independent patients. Biochemical and cultural tests were not suitable for their identification. The mycolic acid pattern analysed by HPLC was different from that of any other mycobacterium. Genotypic characterization, targeting seven housekeeping genes, revealed the presence of microheterogeneity in all of them. Different species were more closely related to the test strains in various regions: the type strain of Mycobacterium moriokaense showed 99.0 % 16S rRNA gene sequence similarity, and 91.5-96.5 % similarity for the remaining six regions. The whole genome sequences of the proposed type strain and that of M. moriokaense presented an average nucleotide identity (ANI) of 82.9 %. Phylogenetic analysis produced poorly robust trees in most genes with the exception of rpoB and sodA where Mycobacterium flavescens and Mycobacterium novocastrense were the closest species. This phylogenetic relatedness was confirmed by the tree inferred from five concatenated genes, which was very robust. The polyphasic characterization of the test strains, supported by the ANI value, demonstrates that they belong to a previously unreported species, for which the name Mycobacterium celeriflavum sp. nov. is proposed. The type strain is AFPC-000207(T) (â= DSM 46765(T)â= JCM 18439(T)).
