ABSTRACT
Arbitrarily primed polymerase chain reaction (AP-PCR) was used to differentiate strains of Legionella pneumophila isolated from different water sources in a resort hotel in Benidorm, Alicante, Spain, where an outbreak of Legionnaires' disease occurred among a group of tourists between 65 and 80 years of age. All isolates were L. pneumophila serogroup 1, subtype Pontiac (Knoxville 1). Five different patterns (P1 to P5) were obtained by AP-PCR. The number of bands per pattern varied between 4 and 11. Patterns P1 and P2 represented 60 and 20% of L. pneumophila isolates, respectively. Since different subpopulations of L. pneumophila coexisted (up to three different AP-PCR patterns were identified in a single room), it was not possible to link an individual L. pneumophila strain to the occurrence of this outbreak.
Subject(s)
Bacterial Typing Techniques , Legionella pneumophila/classification , Random Amplified Polymorphic DNA Technique , Water Microbiology , Water Supply , Antibodies, Monoclonal/immunology , DNA Primers , Electrophoresis, Polyacrylamide Gel , Humans , Legionella pneumophila/genetics , Legionella pneumophila/isolation & purification , Legionnaires' Disease/epidemiology , Legionnaires' Disease/microbiology , Polymerase Chain Reaction , Serotyping , Spain/epidemiologyABSTRACT
A retrospective study to investigate the relationship between the isolation of Chlamydia trachomatis and other factors occurring in 146 patients suffering of non-gonococcal urethritis, has been released 39 being positive for C. trachomatis. The relationship between the isolation of C. trachomatis and previous antibiotic treatment, age, sexual different contacts in the last month and year has been studied. The main feature affecting the isolation is the absence of antimicrobial therapy a previously to the specimen collection and being performed after four days post-contact.
