Biomedical and Biotechnological Potential of Animal Venoms: Bothrops Snake Venoms and their Antimicrobial Applications.

The pursuit of novel treatment alternatives to address the accumulated resistance to antimicrobials over the years has prompted the scientific community to explore biodiversity, particularly animal venom, as a potential source of new antimicrobial drugs. Snake venoms, with their complex mixtures of components, are particularly promising targets for investigation in this regard. The search for novel molecules exhibiting antimicrobial activity against multidrug-resistant strains is of paramount importance for public health and numerous research groups worldwide. High expectations within the healthcare field are supported by the scientific literature, which highlights the potential development of innovative drugs through in vivo and in vitro application, depending on dose titration. Snake venoms and their molecules and peptides offer exponential possibilities for biotechnological applications as antimicrobial agents. However, many uncertainties and unexplored avenues remain, presenting opportunities for discoveries and research.

Functionalized lipid-based drug delivery nanosystems for the treatment of human infectious diseases.

Infectious diseases are still public health problems. Microorganisms such as fungi, bacteria, viruses, and parasites are the main causing agents related to these diseases. In this context, the search for new effective strategies in prevention and/or treatment is considered essential, since current drugs often have side effects or end up, causing microbial resistance, making it a serious health problem. As an alternative to these limitations, nanotechnology has been widely used. The use of lipid-based drug delivery nanosystems (DDNs) has some advantages, such as biocompatibility, low toxicity, controlled release, the ability to carry both hydrophilic and lipophilic drugs, in addition to be easel scalable. Besides, as an improvement, studies involving the conjugation of signalling molecules on the surfaces of these nanocarriers can allow the target of certain tissues or cells. Thus, this review summarizes the performance of functionalized lipid-based DDNs for the treatment of infectious diseases caused by viruses, including SARS-CoV-2, bacteria, fungi, and parasites.

Increased Risk of Fractures and Use of Proton Pump Inhibitors in Menopausal Women: A Systematic Review and Meta-Analysis.

Proton pump inhibitors (PPIs) can directly interfere with osteoclastic function, induce hypergastrinemia, and inhibit calcium absorption, leading to reduced bone mineral density (BMD), a measure of bone metabolism that may be associated with the risk of fractures. The current study involves a systematic review and meta-analysis aimed at assessing the relationship between prolonged use of PPI drugs and fractures in menopausal women. A systematic search and meta-analysis were performed on PubMed, Scopus, and Science Direct databases according to PRISMA guidelines. Two independent reviewers analyzed the articles. The five articles found in the databases, which met the eligibility criteria, covered participants who were menopausal women aged between 56 and 78.5 years, using or not using a PPI for a minimum of 12 months. All studies showed an increase in the rate of fractures related to using PPIs, as an outcome. Prolonged use of PPIs in menopausal women can affect bone metabolism and cause fractures. However, other factors, such as the use of other classes of drugs, obesity, low weight, poor diet, replacement hormones, and comorbidities, should also be considered for assessing the risk of fractures.

Prevalence of Potential Pharmacological Interactions in Patients Undergoing Systemic Chemotherapy in a Tertiary Hospital.

Introduction: Pharmacological interactions are frequently observed in patients with chronic diseases, and their occurrence is proportional to the amount of medication used daily. Patients undergoing chemotherapy treatment commonly have comorbidities, which favor a greater prevalence of polypharmacy, increasing the risk of drug interactions. Therefore, the aim of this study was to estimate the prevalence of drug interactions in patients undergoing intravenous chemotherapy treated at a hospital oncology service in southern Brazil. Methods: This was an observational study with a cross-sectional design that was carried out with the analysis of secondary data obtained through the review of medical records. The population assessed consisted of all cancer patients who received intravenous chemotherapy from October to December 2020. Results: Out of the 297 patients included in the study, 231 (77.8%) had at least 1 potential pharmacological interaction. In total, 1044 drug interactions were found that were classified according to severity, resulting in 18 (1.7%) contraindicated drug-drug interactions (DDI), 699 (67%) severe, 281 (26.9%) moderate, and 46 (4.4%) minor interactions. There was an association between polypharmacy and the prevalence of drug interactions. Conclusion: The results demonstrate that a large percentage of patients undergoing chemotherapy are susceptible to drug interactions. Thus, it is necessary that prescribers consider all drugs used by patients and, when possible, prescribe alternative drugs that have less potential for interaction in order to prevent drug interactions adverse effects and provide a better prognosis for patients.

Natural product-based nanomedicine applied to fungal infection treatment: A review of the last 4 years.

Fungal infections are one of the main public health problems, especially in immunocompromised patients, nosocomial environments, patients with chronic diseases, and transplant recipients. These diseases are increasingly frequent and lethal because the microorganism has a high capacity to acquire resistance to available therapy. The main resistance factors are the emergence of new strains and the uncontrolled use of antifungals. It is, therefore, important to develop new methods that contribute to combating fungal diseases in the clinical area. Natural products have considerable potential for the development of new drugs with antifungal activity, mainly due to their biocompatibility and low toxic effect. This promising antimicrobial activity of natural products is mainly due to the presence of flavonoids, terpenes, and quinones, which explains their antifungal potential. Pharmaceutical nanotechnology has been explored to enhance the delivery, selectivity, and clinical efficacy of these products. Nanotechnological systems provide a safe and selective environment for various substances, such as natural products, improving antifungal activity. However, further safety experiments (in vivo or clinical trials) need to be carried out to prove the therapeutic action of natural products, since they may have undesirable, toxic, and mutagenic effects. Therefore, this review article addresses the main nanotechnological methods using natural products for effective future treatment against the main fungal diseases.

Potential Drug Interactions Between Psychotropics and Intravenous Chemotherapeutics Used by Patients With Cancer.

Introduction: Patients undergoing cancer treatment usually have comorbidities, and psychiatric disorders are commonly seen in these patients. For the treatment of these psychiatric disorders, the use of psychotropic drugs is common, turning these patients susceptible to untoward drug interactions. Therefore, the aim of this study was to estimate the prevalence of clinically relevant drug-drug interactions (DDI) between chemotherapeutic and psychotropic agents in patients with cancer treated at an oncology service in southern Brazil. Methods: An observational epidemiological study with a cross-sectional census-type design was carried out between October and December 2020. The drug-drug interactions were identified through consultation and analysis of the Medscape Drug Interaction Check and Micromedex databases. The interactions were classified as major, when the interaction can be fatal and/or require medical intervention to avoid or minimize serious adverse effects and moderate, when the interaction can exacerbate the patient's condition and/or requires changes in therapy. Results: A total of 74 patients was included in the study among the 194 patients seen in the oncology service during the period studied. A total of 24 (32.4%) DDIs were found, 21 (87.5%) of which were classified as being of major risk and 3 (12.5%) as moderate risk. According to the mechanism of action, 19 (79.1%) were classified as pharmacodynamic interactions and 5 (20.9%) as pharmacokinetic interactions. Conclusion: It was shown that a considerable percentage of patients undergoing intravenous chemotherapy are at risk of pharmacological interaction with psychotropic drugs. Thus, it is essential that the oncologist considers all psychotropic drugs and other drugs used by patients in order to avoid drug-drug interactions.

ATLANTIC POLLINATION: a data set of flowers and interaction with nectar-feeding vertebrates from the Atlantic Forest.

Flowering plant species and their nectar-feeding vertebrates exemplify some of the most remarkable biotic interactions in the Neotropics. In the Brazilian Atlantic Forest, several species of birds (especially hummingbirds), bats, and non-flying mammals, as well as one lizard feed on nectar, often act as pollinators and contribute to seed output of flowering plants. We present a dataset containing information on flowering plants visited by nectar-feeding vertebrates and sampled at 166 localities in the Brazilian Atlantic Forest. This dataset provides information on 1902 unique interactions among 515 species of flowering plants and 129 species of potential vertebrate pollinators and the patterns of species diversity across latitudes. All plant-vertebrate interactions compiled were recorded through direct observations of visits, and no inferences of pollinators based on floral syndromes were included. We also provide information on the most common plant traits used to understand the interactions between flowers and nectar-feeding vertebrates: plant growth form, corolla length, rate of nectar production per hour in bagged flowers, nectar concentration, flower color and shape, time of anthesis, presence or absence of perceptible fragrance by human, and flowering phenology as well as the plant's threat status by International Union for Conservation of Nature (IUCN) classification. For the vertebrates, status of threat by IUCN classification, body mass, bill or rostrum size are provided. Information on the frequency of visits and pollen deposition on the vertebrate's body is provided from the original source when available. The highest number of unique interactions is recorded for birds (1771) followed by bats (110). For plants, Bromeliaceae contains the highest number of unique interactions (606), followed by Fabaceae (242) and Gesneriaceae (104). It is evident that there was geographical bias of the studies throughout the southeast of the Brazilian Atlantic Forest and that most effort was directed to flower-hummingbird interactions. However, it reflects a worldwide tendency of more plants interacting with birds compared with other vertebrate species. The lack of similar protocols among studies to collect basic data limits the comparisons among areas and generalizations. Nevertheless, this dataset represents a notable effort to organize and highlight the importance of vertebrate pollinators in this hotspot of biodiversity on Earth and represents the data currently available. No copyright or proprietary restrictions are associated with the use of this data set. Please cite this data paper when the data are used in publications or scientific events.

The Emerging Landscapes to Drug Delivery Systems for the Treatment of Pancreatic Cancer.

Pancreatic Ductal Adenocarcinoma (PDA) is a highly metastatic tumor, and the liver is its first target, which restricts the use of medications. PDA is considered one of the most aggressive types of cancer in the world, with an extremely short survival time, depending on the stage of diagnosis. In non-surgical cases, chemotherapy alternatives are only effective in 40% to 60% of patients. The low efficiency of treatments occurs mainly due to the complex microenvironment in PDA, leading to chemoresistance to treatments and making it difficult to reach the affected tissue. A very important histological characteristic of PDA is the extremely dense stroma, which leads to low vascularization of tumor tissue. Consequently, the stroma environment causes less drug accumulation in tumor cells, even of selective and/or targeted drugs. Overcoming the stroma's microenvironment is a major challenge for therapies. Moreover, specific genes lead to direct chemoresistance in PDA due to their high progression. In this scenario, nanotechnology appears as an alternative to overcome these clinical challenges concerning two distinct ways: acting on the stroma or/and acting directly on the pancreatic tumor cells. Thus, this review aimed to highlight advances in the application of nanotechnology aiming to open up new landscapes against PDA. There are a huge number of nanoparticles carrying drugs in preclinical and clinical trials for the effective treatment of PDA. These works have been discussed, and based on the current scenario, the future prospects for an efficient treatment of PDA have been proposed.

Highlighting the use of micro and nanoparticles based-drug delivery systems for the treatment of Helicobacter pylori infections.

Due to the high adaptability of Helicobacter pylori and the low targeting specificity of the drugs normally used in pharmacological therapy, the strains are becoming increasingly resistant to these drugs, making it difficult to eradicate the infection. Thus, the search for new therapeutic approaches has been considered urgent. The incorporation of drugs in advanced drug delivery systems, such as nano and microparticles, would allow the improvement of the retention time in the stomach and the prolongation of drug release rates at the target site. Because of this, the present review article aims to highlight the use of micro and nanoparticles as important technological tools for the treatment of H. pylori infections, focussing on the main nanotechnological systems, including nanostructured lipid carriers, liposomes, nanoemulsion, metallic nanoparticles, and polymeric nanoparticles, as well as microtechnological systems such as gastroretentive dosage forms, among them mucoadhesive, magnetic and floating systems were highlighted.

Exploiting drug delivery systems for oral route in the peptic ulcer disease treatment.

Peptic ulcer disease (PUD) is a common condition that is induced by acid and pepsin causing lesions in the mucosa of the duodenum and stomach. The pathogenesis of PUD is a many-sided scenario, which involves an imbalance between protective factors, such as prostaglandins, blood flow, and cell renewal, and aggressive ones, like alcohol abuse, smoking, Helicobacter pylori colonisation, and the use of non-steroidal anti-inflammatory drugs. The standard oral treatment is well established; however, several problems can decrease the success of this therapy, such as drug degradation in the gastric environment, low oral bioavailability, and lack of vectorisation to the target site. In this way, the use of strategies to improve the effectiveness of these conventional drugs becomes interesting. Currently, the use of drug delivery systems is being explored as an option to improve the drug therapy limitations, such as antimicrobial resistance, low bioavailability, molecule degradation in an acid environment, and low concentration of the drug at the site of action. This article provides a review of oral drug delivery systems looking for improving the treatment of PUD.

Exploiting solid lipid nanoparticles and nanostructured lipid carriers for drug delivery against cutaneous fungal infections.

Several types of cutaneous fungal infections can affect the population worldwide, such as dermatophytosis, cutaneous candidiasis, onychomycosis, and sporotrichosis. However, oral treatments have pronounced adverse effects, making the topical route an alternative to avoid this disadvantage. On the other hand, currently available pharmaceutical forms designed for topical application, such as gels and creams, do not demonstrate effective retention of biomolecules in the upper layers of the skin. An interesting approach to optimise biomolecules' activity in the skin is the use of nanosystems for drug delivery, especially solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC), which in the past decade has shown advantages like increased adhesiveness, great occlusive properties and higher biomolecule deposition in stratum corneum when designed for topical application. Considering the demand for more effective therapeutic alternatives and the promising characteristics of SLN and NLC for topical application, the present study sought to gather studies that investigated the potential of using SLN and NLC for the treatment of cutaneous fungal infections. Studies demonstrated that these nanosystems showed optimisation, mostly, of the effectiveness of biomolecules besides other biopharmaceutical properties, in addition to offering potential occlusion and hydration of the applied region.

Current applications of drug delivery nanosystems associated with antimicrobial photodynamic therapy for oral infections.

The oral cavity is colonized by several species of microorganisms that can cause dental caries, periodontal diseases, candidiasis, endodontic infections, and, among other diseases related to the dental field. Conventional treatment consists of mechanical removal associated with systemic administration of antimicrobials, which can cause various side effects and microbial resistance. In this context, alternative therapies have been developed, including Antimicrobial Photodynamic Therapy (aPDT). For the improvement of therapy, the implementation of nanotechnology is very important to optimize the delivery system of the dyes or photosensitizers on biological targets. Besides, this combination provides a non-invasive treatment, better solubility and bioavailability, delivery to the target site, controlled release and protection against external and physical-chemical factors, low side effects, and, unlikely resistant species. Although, there are numerous researches on aPDT and nanotechnology, few review articles based on the combination of these three aspects: nanosystems, aPDT and oral infections are available. For this reason, this article aims to discuss the advances and advantages of this combination. Therefore, this article was divided into different types of nanosystems (organic and inorganic nanoparticles) associated with aPDT bringing a description of it is definitions, properties, and, applications in oral infections.

Nanotechnological strategies for systemic microbial infections treatment: A review.

Systemic infections is one of the major causes of mortality worldwide, and a shortage of drug approaches applied for the rapid and necessary treatment contribute to increase the levels of death in affected patients. Several drug delivery systems based in nanotechnology such as metallic nanoparticles, liposomes, nanoemulsion, microemulsion, polymeric nanoparticles, solid lipid nanoparticles, dendrimers, hydrogels and liquid crystals can contribute in the biological performance of active substances for the treatment of microbial diseases triggered by fungi, bacteria, virus and parasites. In the presentation of these statements, this review article present and demonstrate the effectiveness of these drug delivery systems for the treatment of systemic diseases caused by several microorganisms, through a review of studies on scientific literature worldwide that contributes to better information for the most diverse professionals from the areas of health sciences. The studies demonstrated that the drug delivery systems described can contribute to the therapeutic scenario of these diseases, being classified as safe, active platforms and with therapeutic versatility.

New Silver(I) Coordination Compound Loaded into Polymeric Nanoparticles as a Strategy to Improve In Vitro Anti-Helicobacter pylori Activity.

Helicobacter pylori inhabits the gastric epithelium and can promote the development of gastric disorders, such as peptic ulcers, acute and chronic gastritis, mucosal lymphoid tissue (MALT), and gastric adenocarcinomas. To use nanotechnology as a tool to increase the antibacterial activity of silver I [Ag(I)] compounds, this study suggests a new strategy for H. pylori infections, which have hitherto been difficult to control. [Ag (PhTSC·HCl)2] (NO3)·H2O (compound 1) was synthesized, characterized, and loaded into polymeric nanoparticles (PN1). PN1 had been developed by nanoprecipitation with poly(ε-caprolactone) polymer and poloxamer 407 surfactant. System characterization assays showed that the PNs had adequate particle sizes and ζ-potentials. Transmission electron microscopy confirmed the formation of polymeric nanoparticles (PNs). Compound 1 had a minimum inhibitory concentration for H. pylori of 3.90 µg/mL, which was potentiated to 0.781 µg/mL after loading. The minimum bactericidal concentration of 7.81 µg/mL was potentiated 5-fold to 1.56 µg/mL in PN. Compound 1 loaded in PN1 displayed better activity for H. pylori biofilm formation and mature biofilm. PN1 reduced the toxicity of compound 1 to MRC-5 cells. Loading compound 1 into PN1 inhibited the mutagenicity of the free compound. In vivo, the system allowed survival of Galleria mellonella larvae at a concentration of 200 µg/mL. This is the first demonstration of the antibacterial activity of a silver complex enclosed in polymeric nanoparticles against H. pylori.

Neotropical carnivores: a data set on carnivore distribution in the Neotropics

Mammalian carnivores are considered a key group in maintaining ecological health and can indicate potential ecological integrity in landscapes where they occur. Carnivores also hold high conservation value and their habitat requirements can guide management and conservation plans. The order Carnivora has 84 species from 8 families in the Neotropical region: Canidae; Felidae; Mephitidae; Mustelidae; Otariidae; Phocidae; Procyonidae; and Ursidae. Herein, we include published and unpublished data on native terrestrial Neotropical carnivores (Canidae; Felidae; Mephitidae; Mustelidae; Procyonidae; and Ursidae). NEOTROPICAL CARNIVORES is a publicly available data set that includes 99,605 data entries from 35,511 unique georeferenced coordinates. Detection/non‐detection and quantitative data were obtained from 1818 to 2018 by researchers, governmental agencies, non‐governmental organizations, and private consultants. Data were collected using several methods including camera trapping, museum collections, roadkill, line transect, and opportunistic records. Literature (peerreviewed and grey literature) from Portuguese, Spanish and English were incorporated in this compilation. Most of the data set consists of detection data entries (n = 79,343; 79.7%) but also includes non‐detection data (n = 20,262; 20.3%). Of those, 43.3% also include count data (n = 43,151). The information available in NEOTROPICAL CARNIVORES will contribute to macroecological, ecological, and conservation questions in multiple spatio‐temporal perspectives. As carnivores play key roles in trophic interactions, a better understanding of their distribution and habitat requirements are essential to establish conservation management plans and safeguard the future ecological health of Neotropical ecosystems. Our data paper, combined with other largescale data sets, has great potential to clarify species distribution and related ecological processes within the Neotropics. There are no copyright restrictions and no restriction for using data from this data paper, as long as the data paper is cited as the source of the information used. We also request that users inform us of how they intend to use the data.

Características de pacientes com cárie severa da infância: análise de pacientes atendidos em centro de referência / Characteristics of patients with severe childhood caries: analysis of patients assisted in a reference center

Objetivo: verificar a correlação dos fatores comportamentais na ocorrência de cárie severa. Sujeitos e método: a amostra foi composta de 25 crianças de 6 a 71 meses, atendidas na Clínica Materno Infantil da Faculdade de Odontologia da Universidade de Passo Fundo. O estudo foi realizado em duas etapas: entrevista e exame clínico. A entrevista avaliou os hábitos alimentares e de higiene bucal, as variáveis demográficas e o acesso ao flúor. O exame clínico avaliou a presença de cárie severa (incluindo manchas brancas). Resultados: as principais características das crianças da amostra foram idade superior a 24 meses, menor escolaridade materna, prática da amamentação natural e uso da mamadeira, inclusive com sacarose, além daqueles indivíduos que não realizavam escovação noturna. Não houve diferença estatisticamente significativa na média de ceo-d para nenhum dos fatores em estudo. Conclusão: pode-se concluir que há a necessidade de formar profissionais voltados não apenas ao tratamento, mas também à prevenção das doenças na cavidade oral, como a cárie, mas, para que isso ocorra, é de extrema importância que se conheça o perfil das crianças que necessitam de maior cuidado. (AU)

Objective: verify the correlation of behavioral factors on the occurrence of severe caries. Subjects and method: the sample consisted of 25 children aged six through 71 months, assisted at the Maternal and Child Clinic of the Faculty of Dentistry of the University of Passo Fundo, Brazil. The study was performed in two stages: interview and clinical examination. The interview evaluated food and oral hygiene habits, demographic variables, and access to fluoride. The clinical examination evaluated the presence of severe caries (including white spots). Results: the main characteristics of the children from the sample were age over 24 months, lower maternal level of education, breastfeeding, and bottle-feeding including sucrose, in addition to those who did not perform nighttime brushing. There was no statistically significant difference in the mean of ceo-d for any of the factors studied. Conclusion: it may be concluded that there is a need for training professionals who are focused on both treatment and prevention of diseases in the oral cavity, such as caries. However, to achieve this, it is extremely important to acknowledge the profile of children in need of extended care. (AU)

Características dos óbitos infantis em um munícipio de médio porte da região Nordeste do Brasil / Characteristics of infant deaths in medium towns from brazilian northeast region

Introdução: A mortalidade infantil é um importante indicador de saúde e desenvolvimento dos municípios, revelando importantes aspectos da organização da assistência à saúde. Objetivo: Descrever as características dos óbitos infantis em um munícipio de médio porte da região Nordeste do Brasil. Métodos: Estudo epidemiológico, descritivo, utilizando dados da Declaração de Óbito de crianças com idade de 0 a 364 dias, classificados segundo os critérios de evitabilidade, referentes ao município Jequié, Bahia, entre os anos 2007 a 2012. A análise estatística consistiu no cálculo de frequências absolutas e relativas. Resultados: Entre 2007 e 2012, foram notificados 286 óbitos infantis, dos quais 64,7% foram considerados evitáveis. Entre os 185 óbitos infantis evitáveis, 38,4% se caracterizavam como redutíveis por ações de prevenção, diagnóstico e tratamento precoce. As caraterísticas relacionadas aos óbitos infantis evidenciaram que, entre os óbitos, 53,6% nasceram com baixo peso, 42,1%, prematuros e 63,1% morreram no período neonatal precoce. Conclusões: Os óbitos infantis, especialmente aqueles considerados evitáveis, ainda representam um grave problema de saúde pública. Nesse contexto, analisar os óbitos, quanto aos critérios de evitabilidade, constituiu-se em um instrumento importante na avaliação da qualidade da atenção à saúde materno-infantil, colaborando na elaboração de estratégias de políticas públicas específicas regionais. (AU)

Introduction: Infant mortality is an important health and development indicator of municipalities, revealing important aspects of health care organizations. Objective: To describe the characteristics of infant deaths in a midsized municipality in the Northeast region of Brazil. Methods: This is an epidemiological descriptive study, which has made use of data from death certificates of children aged 0-364 days, selected according to the preventability criteria for the city of Jequié, Bahia, between the years 2007-2012. Statistical analysis included the calculation of absolute and relative frequencies. Results: Between 2007 and 2012, 286 infant deaths were reported, of which 64.7% were considered preventable. Among the 185 preventable child deaths, 38.4% were characterized as reducible by prevention, early diagnosis and treatment. The features related to infant deaths showed that among the deaths, 53.6% had been born with low birth weight; preterm 42.1% and 63.1% died in the early neonatal period. Conclusion: Infant deaths, especially those considered preventable, still represent a serious public health problem. In this context, the analysis of the deaths, as to the preventability criteria, constituted an important tool in assessing the quality of care for maternal and child health, collaborating in the development of regional specific public policy strategies. (AU)

Assessment of the cytotoxic, genotoxic and mutagenic effects of the commercial black dye in Allium cepa cells before and after bacterial biodegradation treatment.

The present study evaluated the cytotoxic, genotoxic and mutagenic actions of different concentrations (50 and 200 µg/L) of BDCP (Black Dye Commercial Product) used by textile industries, before and after bacterial biodegradation, by the conventional staining cytogenetic technique and NOR-banding in Allium cepa cells. Differences in the chromosomal and nuclear aberrations and alterations in the number of nucleoli were observed in cells exposed to BDCP with and without the microbial treatment. The significant frequencies of chromosome and nuclear aberrations noted in the tests with bacterially biodegraded BDCP indicate that the metabolites generated by degradation are more genotoxic than the chemical itself. Losses of genetic material characterize a type of alteration that was mainly associated with the action of the original BDCP, whereas chromosome stickiness, nuclear buds and binucleated cells were the aberrations that were preferentially induced by BDCP metabolites after biodegradation. The significant frequencies of cell death observed in the tests with biodegraded BDCP also show the cytotoxic effects of the BDCP metabolites. The reduction in the total frequency of altered cells after the recovery treatments showed that the test organism A. cepa has the ability to recover from damage induced by BDCP and its metabolites after the exposure conditions are normalized.

Temporomandibular disorder pain improvement with acupuncture: preliminary longitudinal descriptive study / Redução da dor da disfunção temporomandibular com acupuntura: estudo descritivo longitudinal preliminar

BACKGROUND AND OBJECTIVES: Acupuncture is a therapeutic method widely used to manage pain. Temporomandibular disorders have symptoms such as facial pain, pain in temporomandibular region and in masticatory muscles. This study aimed at describing the results obtained in temporomandibular disorder pain intensity improvement in patients treated in a public service with a minimum number of three acupuncture sessions. METHODS: This is a descriptive study with patients with temporomandibular disorders treated in the Dentistry Specialties Center I of the city of Piracicaba (SP) in the period from September 2010 to October 2011. Sample was made up of 31 patients of both genders, aged between 14 and 68 years. Pain was measured by the visual analog scale (VAS), called initial VAS (pre-treatment) and final VAS (post-treatment), respectively in the beginning and end of the acupuncture session. Each patient was submitted to three 20-minute sessions, once a week. RESULTS: Pain was absent (VAS=0) in 67.7% of patients who had pain intensity improvement with iVAS in the first session higher (6.10±2.64) than fVAS in the third session (1.16±1.98). CONCLUSION: With a minimum of three weekly acupuncture sessions it was possible to control temporomandibular disorder pain regardless of the level of initial pain. Its use could contribute to enhance the treatment of such patients in the public service for being a low cost and easy to apply therapy...

JUSTIFICATIVA E OBJETIVOS: Acupuntura é um método terapêutico e seu uso é amplo para o tratamento da dor. As disfunções temporomandibulares apresentam sintomas como dores na face, na região da articulação temporomandibular, nos músculos mastigatórios. O objetivo deste estudo foi descrever os resultados obtidos na redução da intensidade de dor na disfunção temporomandibular em pacientes atendidos no serviço público com um número mínimo de três sessões de acupuntura. MÉTODOS: Trata-se de estudo descritivo de pacientes com disfunção temporomandibular tratados com acupuntura no Centro de Especialidades Odontológicas I do município de Piracicaba (SP), no período de setembro de 2010 a outubro de 2011. A amostra foi de 31 pacientes, de ambos os gêneros, com idade entre 14 e 68 anos. A dor foi mensurada através da escala analógica visual (EAV), denominada EAV inicial (pré-tratamento) e EAV final (pós-tratamento), respectivamente no inicio e ao final da sessão de acupuntura. Cada paciente recebeu três sessões de acupuntura, uma vez por semana com a duração de 20 minutos. RESULTADOS: A dor cessou (EAV=0) em 67,7% dos pacientes que apresentaram redução na intensidade de dor, com EAVi da 1ª sessão maior (6,10±2,64) do que a EAVf da 3ª sessão (1,16±1,98). CONCLUSÃO: Com um número mínimo de três sessões semanais de acupuntura foi possível o controle da dor em pacientes com disfunção temporomandibular independentemente do grau de dor inicial. Sua utilização poderia contribuir para ampliar o atendimento a esses pacientes no serviço público por ser uma prática terapêutica de baixo custo e fácil aplicação...

Efeito hipoalgésico das correntes diadinâmicas de Bernard em indivíduos saudáveis / Hypoalgesic effect of Bernard's diadynamic currents on healthy individuals

JUSTIFICATIVA E OBJETIVOS: As correntes diadinâmicas são correntes alternadas retificadas em ondas completas ou semiondas e foram desenvolvidas por Pierre Bernard. Essas correntes são utilizadas na prática clínica para analgesia e reparação de lesões de tecidos moles; entretanto, sem evidências científicas. O objetivo deste estudo foi investigar o efeito hipoalgésico das correntes diadinâmicas de Bernard em indivíduos saudáveis e o desconforto sensorial de cada corrente. MÉTODO: Foram recrutados 75 voluntários saudáveis, sendo 35 homens e 40 mulheres na faixa etária de 18 a 60 anos. Os voluntários foram distribuídos aleatoriamente em cinco grupos de estudo (15 participantes por grupo), a saber: difásica fixa (DF), monofásica fixa (MF), curtos períodos (CP), longos períodos (LP) e grupo controle (GC). As correntes diadinâmicas foram aplicadas durante 15 minutos no antebraço não dominante e medidas de limiar de dor por pressão foram realizadas na mão e no antebraço antes, durante e 15 minutos após a aplicação das correntes. RESULTADOS: Não houve diferença estatisticamente significante entre os grupos no limiar de dor por pressão na mão no 5° minuto (p = 0,490), 10° minuto (p = 0,590), 15° minuto (p = 0,996) e 30° minuto (p = 0,489). No antebraço também não foram encontradas diferenças significantes entre os grupos no 5° minuto (p = 0,767), 10° minuto (p = 0,439), 15° minuto (p = 0,395) e no 30° minuto (p = 0,915). Não houve diferença estatisticamente significante no desconforto avaliado no 5° minuto (p = 0,087) e 10° minuto (p = 0,055). No entanto, no 15° minuto a corrente CP apresentou menor índice de desconforto quando comparado à corrente MF (p = 0,021). CONCLUSÃO: Não houve diferença no limiar de dor por pressão entre os grupos de estudo.

BACKGROUND AND OBJECTIVES: Diadynamic currents are alternate currents rectified in complete waves or half waves and were developed by Pierre Bernard. These currents are used in the clinical practice for analgesia and soft tissue healing; however, without scientific evidences. This study aimed at investigating the hypoalgesic effect of Bernard's diadynamic currents in healthy individuals and the sensory discomfort of each current. METHOD: Participated in this study 75 healthy volunteers, being 35 males and 40 females aged from 18 to 60 years. Volunteers were randomly distributed in five study groups (15 participants per group), as follows: fixed diphase (DF), fixed monophasic (MF), short periods (CP), long periods (LP) and control group (CG). Diadynamic currents were applied for 15 minutes to the non-dominant forearm and pressure pain thresholds were measured on hand and forearm before, during and 15 minutes after currents application. RESULTS: There has been no statistically significant difference among groups on hand pressure pain threshold in the 5th minute (p = 0.490), 10th minute (p = 0.590), 15th minute (p = 0.996) and 30th minute (p = 0.489). There has also been no significant differences among groups on forearm in the 5th minute (p = 0.767), 10th minute (p = 0.439), 15th minute (p = 0.395) and 30th minute (p = 0.915). There has been no statistically significant difference in discomfort evaluated in the 5th minute (p = 0.087) and 10th minute (p = 0.055). However, in the 15th minute, SP current has shown a lower discomfort index as compared to FM (p = 0.021). CONCLUSION: There has been no difference in pressure pain threshold among studied groups.