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1.
J Biomol Struct Dyn ; 30(1): 89-101, 2012.
Article in English | MEDLINE | ID: mdl-22571435

ABSTRACT

Laccases are multicopper oxidases in which substrate oxidation takes place at the type-1 (T1) copper site. The redox potential (E (0)) significantly varies amongst members of the family and is a key parameter for substrate specificity. Despite sharing highly conserved features at the T1 copper site, laccases span a large range of E (0), suggesting that the influence of the metal secondary coordination sphere is important. In silico analysis of structural determinants modulating the E (0) of Rigidoporus lignosus and other fungal laccases indicated that different factors can be considered. First, the length of the T1 copper coordinating histidine bond is observed to be longer in high E (0) laccases than in low E (0) ones. The hydrophobic environment around the T1 copper site appeared as another important structural determinant in modulating the E (0), with a stronger hydrophobic environment correlating with higher E (0). The analysis of hydrogen bonding network (HBN) around the T1-binding pocket revealed that the amino acids building up the metal binding site strongly interact with neighbouring residues and contribute to the stabilization of the protein folds. Changes in these HBNs that modified the Cu1 preferred coordination geometry lead to an increase of E (0). The presence of axial ligands modulates the E (0) of T1 to different extent. Stacking interactions between aromatic residues located in the second coordination shell and the metal ion coordination histidine imidazole ring have also been identified as a factor that modulates the E (0). The electrostatic interactions between the T1 copper site and backbone carbonyl oxygen indicate that Cu1-CO=NH distance is longer in the high E (0) laccases. In short, the in silico study reported herein identifies several structural factors that may influence the E (0) of the examined laccases. Some of these are dependent on the nature of the coordination ligands at the T1 site, but others can be ascribed to the hydrophobic effects, HBNs, axial ligations, stacking and electrostatic interactions, not necessary directly interacting with the copper metal.


Subject(s)
Fungal Proteins/chemistry , Fungi/enzymology , Laccase/chemistry , Models, Molecular , Amino Acids/chemistry , Binding Sites , Fungal Proteins/metabolism , Histidine/analogs & derivatives , Histidine/chemistry , Hydrogen Bonding , Laccase/metabolism , Ligands , Organometallic Compounds/chemistry , Oxidation-Reduction , Protein Binding , Protein Conformation , Static Electricity
2.
Appl Biochem Biotechnol ; 163(3): 415-22, 2011 Feb.
Article in English | MEDLINE | ID: mdl-20711819

ABSTRACT

The white rot fungus Rigidoporus lignosus produces substantial amounts of extracellular laccase, a multicopper blue oxidase which is capable of oxidizing a wide range of organic substrates. Laccase production can be greatly enhanced in liquid cultures supplemented with various aromatic and phenolic compounds. The maximum enzyme activity was reached at the 21st or 24th day of fungal cultivation after the addition of inducers. The zymograms of extracellular fluid of culture preparation in the presence of inducers, at maximum activity day, revealed two bands with enzymatic activity, called Lac1 and Lac2, having different intensities. Lac2 band shows the higher intensity which changed with the different inducers. Laccase induction can be also obtained by adding to the culture medium olive mill wastewaters, which shows a high content of phenolic compounds.


Subject(s)
Basidiomycota/drug effects , Basidiomycota/enzymology , Hydrocarbons, Aromatic/pharmacology , Laccase/biosynthesis , Phenols/pharmacology , Electrophoresis, Polyacrylamide Gel , Enzyme Induction/drug effects , Extracellular Fluid/drug effects , Extracellular Fluid/metabolism , Industrial Waste/analysis , Isoenzymes/biosynthesis , Laccase/metabolism , Olea/chemistry , Time Factors , Waste Disposal, Fluid
3.
J Biomol Struct Dyn ; 27(4): 501-10, 2010 Feb.
Article in English | MEDLINE | ID: mdl-19916571

ABSTRACT

Laccases are polyphenol oxidases which oxidize a broad range of reducing substrates, preferably phenolic compounds, and their use in biotechnological applications is increasing. Recently, the first X-ray structure of active laccase from white rot fungus Rigidoporus lignosus has been reported containing a full complement of copper ions. Comparison among selected fungal laccases of known 3D structure has shown that the Rigidoporus lignosus laccase has a very high similarity with the Trametes versicolor laccase that, being co-crystallized with 2,5-xylidine, shows a well defined binding pocket for the substrate. Global sequence alignment between Rigidoporus lignosus and Trametes versicolor laccases shows 73% of identity but, surprisingly, there is no identity and neither conservative substitutions between the residues composing the loops directly contacting the 2,5-xylidine. Moreover the structural alignment of these two enzymes identifies in these loops a striking structural similarity proposing the question if 2,5- xylidine may bind in same enzyme pocket. Here we report the protein-ligand docking simulation of 3D structure of Rigidoporus -lignosus laccase and 2,5-xylidine. Docking simulation analyses show that spatial conformation of the two 2,5-xylidine binding pockets, despite differences in the residues directly contacting the ligand, may arrange a similar pocket that allows a comparable accommodation of the inhibitor. To validate these results the binding of 2,5-xylidine in the substrate cavity has been confirmed by kinetic competitive experiments.


Subject(s)
Aniline Compounds/chemistry , Aniline Compounds/metabolism , Basidiomycota/enzymology , Laccase/chemistry , Laccase/metabolism , Amino Acid Sequence , Basidiomycota/chemistry , Basidiomycota/genetics , Binding Sites , Binding, Competitive , Catalytic Domain , Computer Simulation , Crystallization , Crystallography, X-Ray , Laccase/genetics , Models, Molecular , Molecular Sequence Data , Protein Conformation , Sequence Homology, Amino Acid
4.
Chem Res Toxicol ; 18(2): 204-12, 2005 Feb.
Article in English | MEDLINE | ID: mdl-15720124

ABSTRACT

Previous studies have clarified the molecular mechanism of photosensitization on red blood cell membranes induced by some drugs belonging to the class of nonsteroidal antiinflammatory drugs: ketoprofen, naproxen, and diflunisal. This process involves the participation of photodegradation products, free radicals, and reactive oxygen species. The aim of the present paper is to investigate the photohemolytic process using red blood cells of mammalian species, with different membrane phospholipid compositions. Human and bovine red blood cell membranes were selectively enriched with phosphatidylcholine and sphingomyelin. For this purpose, a new approach for phospholipid investigation was undertaken. Moreover, the phototoxic effect was tested with liposomes at different phospholipid compositions. A structure-function relationship between the erythrocyte membrane phospholipid composition and the photohemolytic process induced by the sensitizers can be proposed. Indeed, the different contents of the photoperoxidable double bond and the variable architecture of the membrane bilayer, due to the different phosphatidylcholine and sphingomyelin contents, strongly influence the resistance of the cell to an osmotic shock induced by photogenerated transient species or by the lytic activity of drug photoproducts. The higher content of sphingomyelin, its asymmetric disposition at the outer surface of membrane bilayers, the high level of saturated acyl fatty chains, and the presence of photoperoxidable trans double bonds in the hydrophilic region greatly decrease the fluidity of bilayers and enhance the resistance of the membrane to phototoxic damage. On the other hand, an increase in the content of phosphatidylcholine, which is rich in species with unsaturated acyl fatty chains, decreases the membrane resistance, because these latter can be easily oxidized by drug-photogenerated reactive oxygen species.


Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/chemistry , Anti-Inflammatory Agents, Non-Steroidal/radiation effects , Membranes, Artificial , Phospholipids/chemistry , Photosensitizing Agents/chemistry , Photosensitizing Agents/radiation effects , Animals , Anti-Inflammatory Agents, Non-Steroidal/classification , Cattle , Erythrocytes/chemistry , Humans , Molecular Structure , Photochemistry , Photolysis , Photosensitizing Agents/classification , Time Factors
5.
Biosens Bioelectron ; 20(2): 315-21, 2004 Sep 15.
Article in English | MEDLINE | ID: mdl-15308236

ABSTRACT

Laccases from various sources were tested, and laccase from Rigidoporus lignosus was found to be the most active towards syringaldazine and ABTS, which are typical substrates of this class of enzymes, and towards the phenols found in olive oil mill wastewaters. This laccase was covalently immobilised by carbodiimide chemistry, on a self-assembled monolayer of 3-mercaptopropionic acid deposited on a gold surface. A flow biosensor, using the monolayer of laccase as bioelement and a glassy carbon electrode as amperometric transduction system, was developed. Although the amount of the immobilised enzyme (about 140 ng/cm2 effective surface area) was tiny, the biosensor showed a sensitivity of 3 nA/microM when 1,4-hydroquinone was used as substrate, and a half-life of 35 days. The proposed device permits detection of phenols in aqueous solutions at concentrations in the low micromolar range, i.e. below European Community limits. The biosensor was successfully used to detect phenols in wastewaters from an olive oil mill after minimal sample preparation (incubation of the aqueous sample with sodium borohydride for a few minutes) to suppress the current due to oxidised compounds present in the wastewaters.


Subject(s)
Biosensing Techniques/instrumentation , Electrochemistry/instrumentation , Industrial Waste/analysis , Laccase/chemistry , Phenols/analysis , Phenols/chemistry , Water Pollutants, Chemical/analysis , Biosensing Techniques/methods , Coated Materials, Biocompatible/chemistry , Electrochemistry/methods , Electrodes , Environmental Monitoring/instrumentation , Environmental Monitoring/methods , Enzymes, Immobilized/chemistry , Equipment Design , Equipment Failure Analysis , Laccase/analysis , Laccase/classification , Olive Oil , Plant Oils/analysis , Plant Oils/chemistry , Reproducibility of Results , Sensitivity and Specificity
6.
Mar Environ Res ; 58(2-5): 725-9, 2004.
Article in English | MEDLINE | ID: mdl-15178105

ABSTRACT

The modified nucleoside 7,8-dihydro-8-oxodeoxyguanosine (8-oxo-dG) is an index of oxidative DNA damage. An immunohistochemical approach based on the use of monoclonal antibody 1F7 against 8-oxo-dG was investigated in marine organisms with immunoperoxidase and immunofluorescent detection. Relative staining intensity as a measure of the 8-oxo-dG level was microscopically assessed. After laboratory exposures to benzo[a]pyrene (B[a]P), higher levels of oxidative DNA damage were clearly detected in all treated animals compared to controls. While this method eliminates DNA extraction reducing the processing of biological samples, absolute values are not provided. Further, the method requires only small amounts of tissue and potentially discriminates susceptibility to oxidative damage in different cell types. These results suggest that the assay should have practical applications in marine ecotoxicology.


Subject(s)
Anguilla/genetics , Antibodies, Monoclonal/immunology , Benzo(a)pyrene/toxicity , Bivalvia/genetics , DNA Damage/drug effects , Guanosine/analogs & derivatives , Anguilla/immunology , Animals , Bivalvia/immunology , DNA Damage/genetics , Europe , Fluorescent Antibody Technique , Immunoenzyme Techniques , Immunohistochemistry/methods
7.
Aquat Toxicol ; 67(1): 23-32, 2004 Mar 30.
Article in English | MEDLINE | ID: mdl-15019248

ABSTRACT

7,8-Dihydro-8-oxodeoxyguanosine (8-oxo-dG) is a typical modification of DNA caused by oxygen free radicals and can be an useful biomarker for pollutants inducing oxidative stress. An immunoperoxidase method using monoclonal antibody 1F7 toward 8-oxo-dG was applied to tissues and smeared cells of marine organisms for detection and quantification of oxidative DNA damage in such models. The assay, previously employed on human cells, was assessed for the first time on Mediterranean mussels (Mytilus galloprovincialis) and European eels (Anguilla anguilla), exposed to model pro-oxidant chemicals, namely benzo[a]pyrene (B[a]P) and copper. Quantification of 8-oxo-dG was microscopically carried out and expressed as relative nuclear staining intensity. Higher levels of oxidative DNA damage were detected in the digestive glands of treated mussels compared to controls, while the effect was less pronounced in haemocytes, characterized by more elevated basal levels of 8-oxo-dG. The assay was suitable for detection of 8-oxo-dG also in fish liver sections indicating consistent damage after B[a]P exposure. The main advantage of the immunohistochemical approach is the elimination of DNA extraction which considerably reduces the processing of biological samples. In addition, the assay requires small amounts of frozen tissues or fixed cells for detection of 8-oxo-dG and is potentially able to discriminate variable susceptibility to oxidative stress in different cell types. Although further investigations are required for the improvement and the validation of the assay in field conditions, laboratory exposures provided useful indications on the consistency of the approach and the efficacy of antibody 1F7 in marine organisms for a rapid assessment of pollutant-induced oxidative DNA damage.


Subject(s)
Anguilla/metabolism , Bivalvia/metabolism , Guanosine/analogs & derivatives , Guanosine/toxicity , Immunoenzyme Techniques/methods , Oxidative Stress , Animals , Benzo(a)pyrene , Biomarkers , Copper , DNA Damage/drug effects , Guanosine/metabolism , Italy
8.
Biochim Biophys Acta ; 1601(2): 155-62, 2002 Dec 16.
Article in English | MEDLINE | ID: mdl-12445477

ABSTRACT

The structure and thermal stability of a laccase from Rigidoporus lignosus (Rl) was analysed by Fourier-transform infrared (FT-IR) spectroscopy. The enzyme was depleted of copper atoms, then part of the apoenzyme was re-metalled and these two forms of the protein were analysed as well. The enzymatic activity, lost by the removal of copper atoms, was restored in the re-metalled apoenzyme and resulted similar to that of native protein. The infrared data indicated that the enzyme contains a large amount of beta-sheets and a small content of alpha-helices, and it displayed a marked thermostability showing the T(m) at 92.5 degrees C. The apoenzyme and the re-metalled apoenzyme did not show remarkable differences in the secondary structure with respect to the native protein, but the thermal stability of the apoenzyme was dramatically reduced showing a T(m) close to 72 degrees C, while the re-metalled protein displayed the T(m) at 90 degrees C. These data indicate that copper atoms, beside their role in catalytic activity, play also an important role on the stabilisation of the structure of Rl laccase. About 35% of the polypeptide chain is buried and/or constitutes a particular compact structure, which, beside copper atoms, is probably involved in the high thermal stability of the protein. Another small part of the structure is particularly sensitive to high temperatures and it could be the cause of the loss of enzymatic activity when the temperature is raised above 45-50 degrees C.


Subject(s)
Oxidoreductases/chemistry , Oxidoreductases/metabolism , Polyporales/enzymology , Electron Spin Resonance Spectroscopy , Enzyme Stability , Hot Temperature , Kinetics , Laccase , Protein Denaturation , Spectrophotometry , Spectroscopy, Fourier Transform Infrared/methods , Thermodynamics
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