Effects of Origanum majorana on Breast Cancer Cells: An Alternative to Chemotherapy?

Recent studies have reported several beneficial effects of natural compounds on cancerous cells, highlighting their use for future treatments. These preliminary findings have encouraged experiments with natural substances, such as plant extracts, to examine both cytotoxic and mitogenic effects and find alternative treatments for diseases such as breast cancer. This study examines the effects of microwave-assisted and ethanol maceration of marjoram (Origanum majorana) on MCF-7 breast cancer cell lines and normal breast tissue cell lines used as controls. Marjoram extracts displayed a cytotoxic effect on the MCF-7 cell lines and a mitogenic effect on the control cell lines at the MTS test. The metabolic profiles of MCF-7 and control cell lines were also assessed using the Biolog Phenotype Mammalian Metabolic (PM-M) platform and revealed statistically significant differences in the utilization of energy sources, metabolic activity in the presence of certain ionic species, and responses to metabolic effectors, such as stimulant/catabolic compounds and steroid hormones. Exposure to marjoram extracts exerted positive effects on the MCF-7 cells on the abnormal utilization of energy sources and the responses to metabolic effectors, while no major effects were detected on control cells. These effects were compared to the metabolic impact of the chemotherapeutic agent doxorubicin, which showed profound cytotoxic effects on both cancerous and normal breast cells. In conclusion, our in vitro evidence indicates that marjoram extracts are a promising alternative to chemotherapy in breast cancer since they can successfully eliminate cancerous cells by affecting their metabolic capacity to proliferate without inducing noticeable adverse effects on normal breast tissue.

A psychometric examination of the PAI-SF in persons with recent stroke.

Objective: The present study evaluated the psychometric properties of the Personality Assessment Inventory-Short Form (PAI-SF) for use with patients with recent stroke. Method: Study participants (N = 170) were inpatients in a tertiary hospital in Western Canada admitted to a rehabilitation department who completed a neuropsychological evaluation as part of their care. All participants completed the full-form of the PAI (344 items) and both full- and short-form (160 items) versions were scored from the same protocol. Results: Internal consistency for the PAI-SF scales was assessed by Cronbach's coefficient alpha. Alpha coefficients for clinical scales fell between the range of 0.53 (ANT) to 0.88 (ANX), with three scales (ANT, ALC, and DRG) falling below satisfactory (<0.70). Alpha coefficients were unsatisfactory for validity, treatment, and interpersonal scales. Absolute differences between mean clinical scale t scores between the full and short-form PAI clinical scales ranged from 0.04 (DEP) to 1.18 (MAN). For an individual, absolute differences in scale t scores between the full- and short-forms ranged from 0 to 30 t scores. On average, an individual varied 3.75 t scores between the PAI full- and short-form across all validity, clinical, interpersonal, and treatment scales. Component structure was similar across the full- and short-forms. Conclusions: Findings are somewhat consistent with previous literature on the PAI-SF as the full- and short-forms had minimal differences and similar psychometric properties. However, caution is warranted for the clinical utility for both forms given the lower alpha coefficients and different structure. Only certain clinical scales appear to have strong psychometric properties.

Poly(amine) modified kaolinite clay for VOC capture.

Polyethylenimine (PEI) functionalized kaolinite clay was successfully prepared, characterized, and assessed for the remediation of volatile organic compounds (VOCs) comprising the aldehyde, carboxylic acid, and disulfide functional group classes. A gas chromatographic vapor capture assay evaluated the capability of unmodified and modified clay material to capture representative aldehyde, carboxylic acid, and disulfide VOCs in a laboratory setting. Unmodified kaolinite (Kao) clay was moderately or poorly effective at remediating these VOCs, while the poly(amine) functionalized Kao was capable of capturing VOCs in the vapor phase with reductions up to 100%. Sample cartridge tubes were packed with PEI-functionalized clay in order to assess their ability to reduce the detectable volatile fatty acid load at an open-air rendering plant in a relevant field test for applying these materials in a packed-bed scrubber application. The PEI-Kao packed cartridges were capable of significantly reducing the detectable concentration of volatile fatty acid effluent from the rendering operation. These volatile fatty acids are major contributors to nuisance odors associated with rendering.

Comparison of the Buschke Selective Reminding Test and the California Verbal Learning Test-Second Edition in a stroke population.

There are many tests of learning and memory; however, not all yield analogous results and thus are not interchangeable. This study examined the relationship between the California Verbal Learning Test-Second Edition (CVLT-II) and the Buschke Selective Reminding Test (SRT) in a stroke population. Data from stroke inpatients (N = 102) referred for rehabilitation were collected from medical records. Both tests were administered, as part of a larger neuropsychological assessment. Analyses were conducted on Z-scores from the List Total, Learning, and Long Delay Free Recall (LDFR) subscales of the two tests. SRT and CVLT-II scores were significantly correlated (r = .34 to .52, all p values <.001). However, scores were significantly worse on the SRT compared to the CVLT-II (d = -.90 to - 1.46, all p values <.001). Left hemisphere strokes performed worse than right hemisphere strokes on both tests; however, this trend was not significant. The SRT appeared to be more sensitive to impairment than the CVLT-II; thus, the two tests may not be interchangeable in a stroke population.

Men's Educational Group Appointments in Rural Nicaragua.

Men's preventive health and wellness is largely neglected in rural Nicaragua, where a machismo culture prevents men from seeking health care. To address this issue, a men's educational group appointment model was initiated at a rural health post to increase awareness about hypertension, and to train community health leaders to measure blood pressure. Men's hypertension workshops were conducted with patient knowledge pretesting, didactic teaching, and posttesting. Pretesting and posttesting performances were recorded, blood pressures were screened, and community leaders were trained to perform sphygmomanometry. An increase in hypertension-related knowledge was observed after every workshop and community health leaders demonstrated proficiency in sphygmomanometry. In addition, several at-risk patients were identified and follow-up care arranged. Men's educational group appointments, shown to be effective in the United States in increasing patient knowledge and satisfaction, appear to function similarly in a resource-constrained environment and may be an effective mechanism for reaching underserved men in Nicaragua.

Target-Specific Capture of Environmentally Relevant Gaseous Aldehydes and Carboxylic Acids with Functional Nanoparticles.

Aldehyde and carboxylic acid volatile organic compounds (VOCs) present significant environmental concern due to their prevalence in the atmosphere. We developed biodegradable functional nanoparticles comprised of poly(d,l-lactic acid)-poly(ethylene glycol)-poly(ethyleneimine) (PDLLA-PEG-PEI) block co-polymers that capture these VOCs by chemical reaction. Polymeric nanoparticles (NPs) preparation involved nanoprecipitation and surface functionalization with branched PEI. The PDLLA-PEG-PEI NPs were characterized by using TGA, IR, (1) H NMR, elemental analysis, and TEM. The materials feature 1°, 2°, and 3° amines on their surface, capable of capturing aldehydes and carboxylic acids from gaseous mixtures. Aldehydes were captured by a condensation reaction forming imines, whereas carboxylic acids were captured by acid/base reaction. These materials reacted selectively with target contaminants obviating off-target binding when challenged by other VOCs with orthogonal reactivity. The NPs outperformed conventional activated carbon sorbents.