Communities of P-solubilizing bacteria, fungi and arbuscular mycorrhizal fungi in grass pasture and secondary forest of Paraty, RJ--Brazil.

Communities of P-solubilizing bacteria, fungi and arbuscular mycorrhizal fungi, were evaluated in two different ecosystems. Samplings taken from two areas of Atlantic forest, in Paraty-RJ, Brazil, one with a secondary forest and the other with a grass pasture were studied. Four growth media: GL (glucose and yeast extract), GES (glucose, soil extract, KNO3, CaCl2, MgSO4, NaCl, FeEDTA and micronutrients solution), GAGES (glucose, soil extract, arabinose, glycerol, CaCl2, MgSO4 and NaCl) and GELP (glucose, soil extract, yeast extract, peptone, CaCl2, MgSO4 and NaCl) were evaluated for the isolation of P-solubilizing microorganisms. The identification of P-solubilizing bacteria was based on 16 S rDNA sequence analysis, while the identification of P-solubilizing fungi and arbuscular mycorrhizal fungi was based on morphology. The greatest number of P-solubilizing bacteria was isolated using GL and GELP growth media. The greatest number of P-solubilizing fungi was isolated using GAGES and GES. The bacteria were identified as Enterobacteriaceae and Bacillus sp., while the P-solubilizing fungi were identified as Aspergillus sp. Glomus macrocarpum and Glomus etunicatum were the dominant mycorrhizal fungi in the secondary forest and grass pasture area, respectively.

Communities of P-solubilizing bacteria, fungi and arbuscular mycorrhizal fungi in grass pasture and secondary forest of Paraty, RJ - Brazil

Avaliaram-se as comunidades de bactérias, fungos solubilizadores de fosfato e fungos micorrízicos arbusculares em dois diferentes ecossistemas. Foram feitas amostragens em duas áreas de Mata Atlântica, em Paraty - RJ, Brasil, uma de floresta secundária e outra de pastagem. Foram avaliados quatro meios de cultura: GL (glicose e extrato de levedura), GES (glicose, extrato de solo, KNO3, CaCl2, MgSO4, NaCl, FeEDTA solução de micronutrientes), GAGES (glicose, extrato de solo, arabinose, glicerol, CaCl2, MgSO4 e NaCl) e GELP (glicose, extrato de solo, extrato de levedura, peptona, CaCl2, MgSO4 e NaCl) para isolamento de microrganismos solubilizadores de fosfato. A identificação das bactérias solubilizadoras foi baseada na análise do 16 S rDNA, enquanto que os fungos solubilizadores e os micorrízicos arbusculares foram identificados pela sua morfologia. O maior número de bactérias solubilizadoras foi obtido usando os meios GL e GELP. O maior número de fungos solubilizadores foi obtido com os meios GAGES e GES. As bactérias solubilizadoras foram identificadas como Enterobacteriaceae e Bacillus sp., e os fungos como Aspergillus sp. Glomus macrocarpum e Glomus etunicatum foram as espécies de fungos micorrízicos dominantes nas áreas de floresta secundária e pastagem, respectivamente.