ABSTRACT
AIM: This study aimed to isolate Pseudobrickellia brasiliensis endophytic bacteria and evaluate the production of hydrolytic enzymes and antibiotics by these bacterial strains. The study also measured the antibacterial activity of P. brasiliensis. METHODS AND RESULTS: Thirteen endophytic bacteria strains were isolated from stem and leaf fragments of P. brasiliensis. Extracellular enzyme production by the isolated endophytic bacteria was evaluated in an agar plate-based assay. The highest protease production was achieved by Bacillus subtilis P4 in alkaline medium. Antimicrobial activity of endophytic bacteria and P. brasiliensis extracts was investigated using microbroth dilution. An MIC value of 1000 µg ml-1 against Pseudomonas aeruginosa was found for B. subtilis P3, B. subtilis P5, Pseudomonas sp. P8 and Pseudomonas sp. P12. Leaf extract of P. brasiliensis showed the highest antibacterial activity against P. aeruginosa, with an MIC value of 0·781 mg ml-1 . CONCLUSIONS: Pseudobrickellia brasiliensis is a source of bacterial endophytes, which can produce antibacterial compounds and enzymes. This work also demonstrated the antibacterial potential of P. brasiliensis. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first study that revealed the antibacterial activity of P. brasiliensis and bioactive metabolite production by P. brasiliensis endophytic bacteria.
Subject(s)
Asteraceae/microbiology , Endophytes/isolation & purification , Plants, Medicinal/microbiology , Anti-Bacterial Agents/metabolism , Anti-Bacterial Agents/pharmacology , Bacillus subtilis/isolation & purification , Bacillus subtilis/metabolism , Bacteria/drug effects , Bacterial Proteins/metabolism , Endophytes/metabolism , Microbial Sensitivity Tests , Peptide Hydrolases/metabolism , Plant Extracts/pharmacologyABSTRACT
Analyses of 16S rDNA genes were used to identify the microbiota isolated from the mucus of the zoanthid Palythoa caribaeorum at Porto de Galinhas on the coast of Pernambuco State, Brazil. This study is important as the first report of this association, because of the potential biotechnological applications of the bacterium Alcanivorax dieselolei, and as evidence for the presence of a hydrocarbon degrading bacterium in a reef ecosystem such as Porto de Galinhas.
Subject(s)
Alcanivoraceae/genetics , Anthozoa/microbiology , Mucus/microbiology , Alcanivoraceae/physiology , Animals , DNA, Bacterial/genetics , Polymerase Chain Reaction , RNA, Ribosomal, 16S/geneticsABSTRACT
Analyses of 16S rDNA genes were used to identify the microbiota isolated from the mucus of the zoanthid Palythoa caribaeorum at Porto de Galinhas on the coast of Pernambuco State, Brazil. This study is important as the first report of this association, because of the potential biotechnological applications of the bacterium Alcanivorax dieselolei, and as evidence for the presence of a hydrocarbon degrading bacterium in a reef ecosystem such as Porto de Galinhas.
Análises dos genes 16S rDNA foram empregadas para identificar a microbiota isolada do muco do zoantídeo Palythoa caribaeorum de Porto de Galinhas, litoral do estado de Pernambuco, Brasil. Este estudo é importante pelo ineditismo dessa associação, pelas relevantes aplicações biotecnológicas da bactéria Alcanivorax dieselolei e pela indicação da presença de uma bactéria degradadora de hidrocarbonetos em um ecossistema recifal como o de Porto de Galinhas.
Subject(s)
Animals , Alcanivoraceae/genetics , Anthozoa/microbiology , Mucus/microbiology , Alcanivoraceae/physiology , DNA, Bacterial/genetics , /genetics , Polymerase Chain ReactionABSTRACT
The degree of anterior curvature was measured on 30 cadaveric human femora, of unknown age and sex, by a spherometer. The mean anterior curvature radius was (1381.28 +/- 118.63) mm with a considerable interspecimen variation, the maximal value was 3262.66 mm and the minimal 695.54 mm.
Subject(s)
Femur/anatomy & histology , HumansABSTRACT
Transplantes heterotopicos de figado foram executados em grupo de 13 caes pre-sensibilizados atraves de enxertos sucessivos de pele dos caes doadores. A sensibilizacao era definida atraves de testes de microlinfocitotoxicidade. Em sete de 13 caes observou-se rejeicao hiperaguda caracterizada por bloqueio do retorno venoso hepatico resistente ao tratamento com isoproterenol. Apenas um dos caes do grupo controle faleceu em decorrencia de erro tecnico. Os estudos de evolucao clinica e laboratorial dos caes do grupo sensibilizado revelaram que os transplantes de figado nao rejeitados de forma hiperaguda apresentavam evolucao ulterior semelhante a dos caes do grupo controle
