ABSTRACT
In 2011, an outbreak of severe vesicular disease occurred in the state of Pará, Amazon region. Besides proliferative or verrucous lesions, cattle showed atypical clinical signs such as diarrhea and leading to death. The animals were submitted to clinical, pathological and molecular diagnosis, and laboratory tests have confirmed the presence of Pseudocowpox virus (PCPV), a Parapoxvirus genus member, and have also found Bovine viral diarrhea virus-1 (BVDV-1), probably causing persistent infection. The results of molecular diagnostics, followed by sequencing data demonstrated the circulation of both viruses (PCPV and BVDV-1) in an area previously affected by another poxvirus, as Vaccinia virus.The cocirculation between PCPV and BVDV-1 indicates a major concern for animal health because the clinical presentation can be a severe disease. This is the first detection of PCPV in the Brazilian Amazon.
Subject(s)
Bovine Virus Diarrhea-Mucosal Disease/virology , Cattle Diseases/epidemiology , Cattle Diseases/virology , Coinfection/veterinary , Disease Outbreaks/veterinary , Poxviridae Infections/veterinary , Pseudocowpox Virus/isolation & purification , Animals , Antigens, Viral/blood , Antigens, Viral/genetics , Bovine Virus Diarrhea-Mucosal Disease/diagnosis , Bovine Virus Diarrhea-Mucosal Disease/epidemiology , Bovine Virus Diarrhea-Mucosal Disease/physiopathology , Brazil/epidemiology , Cattle , Cattle Diseases/diagnosis , Coinfection/diagnosis , Coinfection/epidemiology , Coinfection/virology , Diarrhea , Diarrhea Virus 1, Bovine Viral/genetics , Phylogeny , Poxviridae Infections/diagnosis , Poxviridae Infections/epidemiology , Poxviridae Infections/virology , Pseudocowpox Virus/genetics , Real-Time Polymerase Chain Reaction , Sequence Analysis, DNAABSTRACT
In 2010, vaccinia virus caused an outbreak of bovine vaccinia that affected dairy cattle and rural workers in Pará State, Brazil. Genetic analyses identified the virus as distinct from BeAn58058 vaccinia virus (identified in 1960s) and from smallpox vaccine virus strains. These findings suggest spread of autochthonous group 1 vaccinia virus in this region.
Subject(s)
Disease Outbreaks , Vaccinia virus/classification , Vaccinia virus/genetics , Vaccinia/epidemiology , Vaccinia/veterinary , Zoonoses/epidemiology , Animals , Brazil/epidemiology , Cattle , Cattle Diseases/epidemiology , Cattle Diseases/virology , Genes, Viral , Geography, Medical , Humans , Phylogeny , Vaccinia/pathologyABSTRACT
Nove casos de encefalomielite equina foram estudados na Ilha de Marajó, estado do Pará, Brasil. Os equinos apresentavam dificuldade em se manter em estação, andavam em círculo, tinham acentuada depressão, pálpebras cerradas, paralisia da língua, tremores musculares, bruxismo, anorexia e desidratação. Alguns apresentavam diminuição dos reflexos auricular, palpebral, de ameaça, diminuição do tônus da língua e taquicardia. Posição de auto-auscultação foi observada com frequência. Os animais muitas vezes eram encontrados apoiados em troncos e cercas para se manterem em estação. À necropsia verificou-se hemorragia das leptomeninges e da medula, alguns apresentaram ainda aderência das leptomeninges. À histopatologia verificou-se encefalite difusa que afetava principalmente a substância cinzenta, com meningite e coroidite. Foi observada perivasculite mononuclear. Em dois equinos identificou-se o vírus da encefalomielite equina Leste pela reação de Semi-Nested transcrição reversa de polimerase em cadeia (Semi-Nested RT-PCR).
Nine cases of equine encephalomyelitis on Marajó Island, state of Pará, Brazil, were studied. The affected horses had difficulty to stand, walked in circles, with marked depression, closed eyelids, tongue paralysis, muscle tremors, bruxism, anorexia and dehydration. Some had their ear and eyelid reflexes diminished, decreased tongue tone and tachycardia; laid down frequently they kept their head on the chest. Often they were seen resting their head on tree trunks or fences. At necropsy, hemorrhages of the meninges and spinal cord, and in some animals also adhesion of the meninges were found. Histologically there was diffuse encephalitis affecting mainly the gray matter, with meningitis and choroiditis. Presence of perivascular cuffs consisting of mononuclear inflammatory cells was observed. From two horses the Eastern equine encephalitis virus was identified by semi-nested reverse transcription polymerase chain reaction (semi-nested RT-PCR).
