ABSTRACT
Squamous cell cancer of the oral cavity is a pathology with low survival rates for patients who suffer from it. It is suggested that this may be a consequence of its recurrence and resistance to treatment. It has been suggested that progenitor tumor cells due to their self-renewal, tumor initiation, migration and metastasis properties may be responsible for the maintenance and renewal of the tumor. However, there is still no consensus on the true participation of these cells and their identification and characterization is still an experimental challenge. In this study, it was possible to identify in situ, using fluorescence microscopy, different markers of progenitor tumor cells: Tumour-Related Antigen [TRA] -1-60, Stage- Specific Embryonic Antigen-4 [SSEA-4], SOX2, NANOG and OCT4, The presence of these markers suggests the participation of tumor progenitor cells in the evolution of this type of cancer, which opens up new therapeutic avenues for the possible treatment of this tumor in order to improve prognosis, survival rate and quality of life of the patient
El cancer escamocelular de cavidad oral es una patología con bajas tasas de sobrevivencia para los pacientes que lo padecen. Se plantea que esto puede ser consecuencia de su recurrencia y resistencia al tratamiento. Se ha planteado que las células tumorales progenitoras por sus propiedades de auto renovación, iniciación tumoral, migración y metástasis pueden ser lasresponsables de la manutención y renovación del tumor. Sin embargo, aun no existe un consenso sobre la verdadera participación de estas células y su identificación y caracterización es aun un reto experimental. En este estudio se lograron identificar in situ, mediante microscopia defluorescencia, diferentes marcadores de células tumorales progenitoras: Tumour-Related Antigen [TRA]-1-60, Stage-Specific Embryonic Antigen-4 [SSEA-4], SOX2, NANOG y OCT4, La presencia de estos marcadores sugiere la participación de las célulasprogenitoras tumorales en la evolución de este tipo de cáncer, lo que abre nuevas vías terapéuticas para el posible tratamiento de este tumor con el fin de mejorar el pronostico, tasa de sobrevivencia y calidad de vida del paciente.
ABSTRACT
New evidence suggests that glycan expression in placental cells of women with invasive disorders of pregnancy differs from that in normal pregnant women. Hypothesizing that modifications of glycan expression could account for the course of preeclampsia, we established placental villous histocultures and compared glycan expression in women with preeclampsia with that in normal pregnant women and also in syncytialized BeWo cells, and we tested the effect of glycan expression on the functional phenotypes of circulating natural killer (NK) cells. Histocultures of third-trimester placentae from women with preeclampsia and full-term placentae from healthy pregnant women and BeWo choriocarcinoma cells were assessed for the expression of terminal glycans by lectin-binding assays. Circulating NK cells from nonpregnant healthy donors were tested in vitro for their cytotoxic activity and intracellular cytokine content. Histocultures from women with preeclampsia expressed significantly more mannose than did those from healthy pregnant women. Both histocultures and BeWo cells expressed terminal fucose, mannose, sialic acid, and N -acetylgalactosamine, although mean fluorescence intensity (MFI) expression was lower in choriocarcinoma cells than in cells from histocultures. Cocultures of circulating NK cells with K562 target cells resulted in a dose-dependent cytotoxicity effect, but the use of BeWo cells as target reduced cytotoxic activity; this reduction was not affected by syncytialization. Histocultures of placental villous tissue of women with preeclampsia expressed high levels of terminal mannose. We proposethat placental glycans may modulate the functional activity of circulating NK cells in the context of systemic inflammatory response in preeclampsia.
