ABSTRACT
We report an outbreak of abortion due to equine herpesvirus (EHV) in 5 mares between 9 and 11 months of gestation, from a herd of 22 Thoroughbred mares. Equine herpesvirus was isolated from extracts of the liver, spleen and thymus but not from the lungs of a 9-month fetus grown in Rabbit Kidney (RK13) cells. The virus was identified by electron microscopy, where virus particles could be seen in the nucleus of infected cells, and by the fluorescent antibody technique with polyclonal antibodies against the whole virus. Anamnesis, necropsy, histopathology, bacteriology, and virology data suggest that the abortions reported in this paper were due to equine herpesvirus.
Subject(s)
Abortion, Veterinary/virology , Herpesviridae Infections/veterinary , Herpesvirus 1, Equid/isolation & purification , Horse Diseases/virology , Abortion, Veterinary/epidemiology , Animals , Antibodies, Viral , Brazil/epidemiology , Female , Fetus/pathology , Fetus/virology , Herpesviridae Infections/epidemiology , Herpesviridae Infections/prevention & control , Herpesviridae Infections/virology , Herpesvirus 1, Equid/immunology , Herpesvirus 1, Equid/ultrastructure , Horse Diseases/epidemiology , Horses , Liver/pathology , Liver/virology , Microscopy, Electron , Pregnancy , Spleen/pathology , Spleen/virology , Thymus Gland/pathology , Thymus Gland/virologyABSTRACT
We report an outbreak of abortion due to equine herpesvirus (EHV) in 5 mares between 9 and 11 months of gestation, from a herd of 22 Thoroughbred mares. Equine herpesvirus was isolated from extracts of the liver, spleen and thymus but not from the lungs of a 9-month fetus grown in Rabbit Kidney (RK13) cells. The virus was identified by electron microscopy, where virus particles could be seen in the nucleus of infected cells, and by the fluorescent antibody technique with polyclonal antibodies against the whole virus. Anamnesis, necropsy, histopathology, bacteriology, and virology data suggest that the abortions reported in this paper were due to equine herpesvirus.
Subject(s)
Animals , Female , Pregnancy , Abortion, Veterinary , Horse Diseases/virology , Herpesvirus 1, Equid , Herpesviridae Infections/veterinary , Abortion, Veterinary , Antibodies, Viral , Spleen/pathology , Spleen/virology , Brazil , Horse Diseases/epidemiology , Fetus , Liver/pathology , Liver/virology , Herpesvirus 1, Equid , Horses , Herpesviridae Infections/epidemiology , Herpesviridae Infections/prevention & control , Herpesviridae Infections/virology , Microscopy, Electron , Thymus GlandABSTRACT
We describe an acute outbreak of balanoposthitis in bulls at an artificial insemination station in South Brazil. Bovine herpesvirus was isolated from preputial swabs in the Crandell feline kidney cell line and secondary fetal bovine lung cells and identified using the fluorescent antibody technique and electron microscopy. Polyclonal antibodies against the whole virus were used for identification of the bovine herpesvirus with the fluorescent antibody technique. Herpesvirus particles could be seen in the infected cells by electron microscopy. Eleven bulls had clinical signs resembling balanoposthitis and nine yielded virus.
Subject(s)
Balanitis/veterinary , Cattle Diseases/microbiology , Herpesviridae Infections/veterinary , Animals , Balanitis/diagnosis , Brazil , Cattle , Herpesviridae/isolation & purification , MaleABSTRACT
We describe an acute outbreak of balanoposthitis in bulls at an artificial insemination station in South Brazil. Bovine herpesvirus was isolated from preputial swabs in the Crandell feline kidney cell line and secondary fetal bovine lung cells and identified using the fluorescent antibody technique and electron microscopy. Polyclonal antibodies against the whole virus were used for identification of the bovine herpesvirus with the fluorescent antibody technique. Herpesvirus particles could be seen in the infected cells by electron microscopy. Eleven bulls had clinical signs resembling balanoposthitis and nine yelded virus
