Relationship of tobacco smoking with GSTM1 gene polymorphism in laringeal cancer.

This paper aimed to analyze the association of polymorphism of GSTM1 0/0 genotype with laryngeal cancer along a hospital based case-control study. Polymorphisms of GSTM1 0/0 of samples from 36 patients with laryngeal cancer and 35 healthy controls were detected by PCR method. The reaction used as GSTM1 primers, using the sequence sense: 5'-CTGCCCTACTTGGATTGATGGG-3' and antisense: 5'-TGGATTGTAGCAGATCATGC-3'. N Acetyl transferase 1 (NAT1) gene using the primers sense: 5'-TAAAAGTAAAATGATTTGCTTTCG-3' and antisense: 5'- GCTTTCTAGCATAAATCACCAA-3' was used as internal positive control. Two sided 2 and multivariation analysis were used to analyse the results. The proportions of GSTM1 deleted genotype in cases and controls were 47.2% and 54.3%, respectively. There was significant increment of GSTM 0/0 genotype frequency in moderate smokers group of patients compared to control (P=0.033, OR= 4.78, 95% CI = 1.30-7.13). We conclude that GSTM1 deleted genotype may be a genetic susceptibility marker for laryngeal cancer whose exposed to low doses carcinogens. The absence of this enzyme seems to have a role in the development of laryngeal cancer, in which the mechanism still needs further investigation.

A comparison of nasal resistance in white Caucasians and blacks.

Total nasal resistance was measured in 42 white caucasian and 32 black healthy adults by both anterior and posterior rhinomanometry after using a nasal decongestant. The nasal airway resistance was found to be lower in the blacks compared to the caucasians by both anterior and posterior methods. The mean total nasal airway resistance was 0.136 Pa/ cm3/s in the blacks and 0.179 Pa/cm3/s in the caucasians by the anterior method, and 0.134 Pa/cm3/s and 0.161 Pa/cm3/s, respectively, by the posterior method. These differences were highly statistically significant.