A New Training Load Quantification Method at Supramaximal Intensity and Its Application in Injuries Among Members of an International Volleyball Team.

ABSTRACT: Bouzigues, T, Maurelli, O, Imbach, F, Prioux, J, and Candau, R. A new training load quantification method at supramaximal intensity and its application in injuries among members of an international volleyball team. J Strength Cond Res XX(X): 000-000, 2024-The quantification of training loads (TLs) is essential for optimizing jump performance and reducing the occurrence of injuries. This study aimed to (a) propose a new method for quantifying TLs in explosive exercises, (b) determine the nature of the relationship between TLs dynamics and injury occurrence, and (c) assess a TL critical for training schedule purposes, above which the risk of injury occurrence becomes unacceptable. This study was conducted with 11 male volleyball players on a national team during a 5-month international competitive period. The proposed new method for quantifying TLs is based on a weighting factor applied to relative jumping intensities, determined by the number of sustainable jumps and their intensities measured by G-Vert accelerometer. The relationship between TLs dynamics and injury occurrence was assessed using a variable dose-response model. A high coefficient of determination was found between the maximum number of jumps possible and their intensities ( r2 = 0.94 ± 0.14, p < 0.001), indicating a strong physiological relationship between jumping intensities and the constraints imposed. The occurrence of injury was dependent on TLs dynamics for 2 players ( r2 = 0.26 ± 0.01; p < 0.001). The TL critical corresponded to 11 jumps over 80% of maximum jump height during games and approximately 130 jumps at <80% of maximal jump height. The present study proposes a new approach for quantifying supramaximal exercises and provides tools for training schedules and the prevention of volleyball injuries.

Differences in training load, jump performance and injury occurrence in elite youth volleyball players.

BACKGROUND: External and internal training load are used to monitor training effects in volleyball. Occurrence of injuries in volleyball is dependent of training loads and state of fitness but also playing positions and gender. This study aims to investigate the impact of gender and playing positions on injury occurrence among young volleyball players, considering both training loads and fitness levels. METHODS: Conducted from September 2021 to May 2022, this study involved 37 elite young volleyball players, comprising 16 female (176.8±3.6 cm; 65.3±5.7 kg; 13.9±1.1 years old) and 21 males (189.6±7.3 cm; 77.4±9.5 kg; 14.7±1.2 years old). G-Vert accelerometer was used to quantify training load. During these sessions, RPE, state of fitness and occurrence of injuries, were collected using a daily questionnaire. RESULTS: The primary findings indicate that males demonstrated a higher number of jumps, mean intensity, mean training load per session, and reported higher fitness levels compared to females (P<0.001). However, females were more injured than males (P<0.001). Setters were identified as the players with the highest jump frequency, albeit at lower heights and intensities than their counterparts (P<0.001). Among males, middle blockers exhibited the highest mean intensity and training load per session (P<0.01). CONCLUSIONS: The elevated frequency of injuries and a worse reported fitness levels among females, despite lower training loads, suggests a potential deficiency in physical preparation among young women, particularly in terms of their ability to perform repeated high-intensity jumps.

Active recovery vs hot- or cold-water immersion for repeated sprint ability after a strenuous exercise training session in elite skaters.

This study compared the acute effects of three recovery methods: active recovery (AR), hot- and cold-water immersion (HWI and CWI, respectively), used between two training sessions in elite athletes. Twelve national-team skaters (7 males, 5 females) completed three trials according to a randomized cross-over study. Fifteen minutes after an exhaustive ice-skating training session, participants underwent 20 min of HWI (41.1 ± 0.5°C), 15 min of CWI (12.1 ± 0.7°C) or 15 min of active recovery (AR). After 1 h 30 min of the first exercise, they performed a repeated-sprint cycling session. Average power output was slightly but significantly higher for AR (767 ± 179 W) and HWI (766 ± 170 W) compared to CWI (738 ± 156 W) (p = 0.026, d = 0.18). No statistical difference was observed between the conditions for both lactatemia and rating of perceived exertion. Furthermore, no significant effect of recovery was observed on the fatigue index calculated from the repeated sprint cycling exercises (p > 0.05). Finally, a positive correlation was found between the average muscle temperature measured during the recoveries and the maximal power output obtained during cycling exercises. In conclusion, the use of CWI in between high-intensity training sessions could slightly impair the performance outcomes compared to AR and HWI. However, studies with larger samples are needed to confirm these results, especially in less trained athletes.

Feasibility and early effects of bed-cycling eccentric training: Potential clinical applications.

PURPOSE: This study aimed to evaluate feasibility and early effects of moderate intensity bed-cycling eccentric training on healthy individuals, and establish whether this training modality could be implemented into bedridden patients' routine care. METHODS: Longitudinal study with prepost exercise intervention measurements. The development of a bed-adapted eccentric ergometer allowed to conduct five training sessions during 3 weeks at increasing intensity on 11 healthy individuals. Force-speed relationship, maximal voluntary knee extension force and neural activation of subjects were evaluated before and after the programme. RESULTS: Five training sessions were sufficient to decrease the rate of perceived exertion whereas eccentric power output increased (+40%). After training, maximal voluntary isometric contraction force measured during knee extension had significantly improved in all subjects, with a mean increase of 17%. Maximal cycling power was also significantly higher (+7%) after the training programme. CONCLUSION: Taken together, these results show that moderate load eccentric bed cycling (i) was feasible and efficient, (ii) did not generate excessive individual perception of effort during exercise nor develop major muscular or joint pain after training and (iii) allowed early force and power gains in healthy subjects.

Using global navigation satellite systems for modeling athletic performances in elite football players.

This study aims to predict individual Acceleration-Velocity profiles (A-V) from Global Navigation Satellite System (GNSS) measurements in real-world situations. Data were collected from professional players in the Superleague division during a 1.5 season period (2019-2021). A baseline modeling performance was provided by time-series forecasting methods and compared with two multivariate modeling approaches using ridge regularisation and long short term memory neural networks. The multivariate models considered commercial features and new features extracted from GNSS raw data as predictor variables. A control condition in which profiles were predicted from predictors of the same session outlined the predictability of A-V profiles. Multivariate models were fitted either per player or over the group of players. Predictor variables were pooled according to the mean or an exponential weighting function. As expected, the control condition provided lower error rates than other models on average (p = 0.001). Reference and multivariate models did not show significant differences in error rates (p = 0.124), regardless of the nature of predictors (commercial features or extracted from signal processing methods) or the pooling method used. In addition, models built over a larger population did not provide significantly more accurate predictions. In conclusion, GNSS features seemed to be of limited relevance for predicting individual A-V profiles. However, new signal processing features open up new perspectives in athletic performance or injury occurrence modeling, mainly if higher sampling rate tracking systems are considered.

The Use of Fitness-Fatigue Models for Sport Performance Modelling: Conceptual Issues and Contributions from Machine-Learning.

The emergence of the first Fitness-Fatigue impulse responses models (FFMs) have allowed the sport science community to investigate relationships between the effects of training and performance. In the models, athletic performance is described by first order transfer functions which represent Fitness and Fatigue antagonistic responses to training. On this basis, the mathematical structure allows for a precise determination of optimal sequence of training doses that would enhance the greatest athletic performance, at a given time point. Despite several improvement of FFMs and still being widely used nowadays, their efficiency for describing as well as for predicting a sport performance remains mitigated. The main causes may be attributed to a simplification of physiological processes involved by exercise which the model relies on, as well as a univariate consideration of factors responsible for an athletic performance. In this context, machine-learning perspectives appear to be valuable for sport performance modelling purposes. Weaknesses of FFMs may be surpassed by embedding physiological representation of training effects into non-linear and multivariate learning algorithms. Thus, ensemble learning methods may benefit from a combination of individual responses based on physiological knowledge within supervised machine-learning algorithms for a better prediction of athletic performance.In conclusion, the machine-learning approach is not an alternative to FFMs, but rather a way to take advantage of models based on physiological assumptions within powerful machine-learning models.

Training load responses modelling and model generalisation in elite sports.

This study aims to provide a transferable methodology in the context of sport performance modelling, with a special focus to the generalisation of models. Data were collected from seven elite Short track speed skaters over a three months training period. In order to account for training load accumulation over sessions, cumulative responses to training were modelled by impulse, serial and bi-exponential responses functions. The variable dose-response (DR) model was compared to elastic net (ENET), principal component regression (PCR) and random forest (RF) models, while using cross-validation within a time-series framework. ENET, PCR and RF models were fitted either individually ([Formula: see text]) or on the whole group of athletes ([Formula: see text]). Root mean square error criterion was used to assess performances of models. ENET and PCR models provided a significant greater generalisation ability than the DR model ([Formula: see text], [Formula: see text], [Formula: see text] and [Formula: see text] for [Formula: see text], [Formula: see text], [Formula: see text] and [Formula: see text], respectively). Only [Formula: see text] and [Formula: see text] were significantly more accurate in prediction than DR ([Formula: see text] and [Formula: see text]). In conclusion, ENET achieved greater generalisation and predictive accuracy performances. Thus, building and evaluating models within a generalisation enhancing procedure is a prerequisite for any predictive modelling.

Does the interference phenomenon affect strength development during same-session combined rehabilitation program in hemodialysis patients?

INTRODUCTION: This study aimed to assess if an interference effect could blunt the neuromuscular gains induced by a same-session combined rehabilitation in hemodialysis (HD) patients. METHODS: Patients exercised twice a week, for 16 weeks, over their HD sessions. They were either always trained with resistance and endurance exercises (continuous training, "CONT") or alternatively with 1 week of resistance alternated with 1 week of endurance (discontinuous training, "DISC"). Adherence and workload were continuously recorded. Short Physical Performance Battery (SPPB) score, one-leg balance test, and handgrip and quadriceps strength were evaluated before and after training intervention. RESULTS: Adherence to both programs was high (>90%). SPPB score had significantly improved (CONT: +1.5 point, DISC: +1.2 pt, p < 0.001), like one-leg balance test (CONT: +3.7 s, DISC: +5.5 s, p < 0.05), handgrip strength of exercised (CONT: +5.5 kg, DISC: +5.6 kg, p < 0.001) and of nonexercised arm (CONT: +4.4 kg, DISC: +2.8 kg, p < 0.01) as well as maximal quadriceps strength (+22 N·m for dominant and +29 N·m for nondominant leg in both groups, p < 0.001) bearing no difference between the trainings. CONCLUSION: Same-session combined training does not induce an interference effect in HD patients and temporal separation of exercises does not optimize strength gains. These practical data may be relevant for clinicians and practitioners to alternate endurance and resistance exercises.

Modelling performance with exponential functions in elite short-track speed skaters.

Mathematical models are used to describe and predict the effects of training on performance. The initial models are structured by impulse-type transfer functions, however, cellular adaptations induced by exercise may exhibit exponential kinetics for their growth and subsequent dissipation. Accumulation of exercise bouts counteracts dissipation and progressively induces structural and functional changes leading to performance improvement. This study examined the suitability of a model with exponential terms (Exp-Model) in elite short-track speed (ST) skaters. Training loads and performance evolution from fifteen athletes (10 males, 5 females) were previously collected over a 3-month training period. Here, we computed the relationship between training loads and performance with Exp-Model and compared with previous results obtained with a variable dose-response model (Imp-Model). Exp-Model showed a higher correlation between actual and modelled performances (R2 = 0.83 ± 0.08 and 0.76 ± 0.07 for Exp-Model and Imp-Model, respectively). Concerning model parameters, a higher τA1 (time constant for growth) value was found (p = 0.0047; d = 1.4; 95% CI [0.4;1.9]) in males compared to females with Exp-model, suggesting that females have a faster adaptative response to training loads. Thus, according to this study, Exp-model may better describe training adaptations in elite ST athletes than Imp-Model.

Influence of post-exercise hot-water therapy on adaptations to training over 4 weeks in elite short-track speed skaters.

This study aimed to investigate the effects of regular hot water bathing (HWB), undertaken 10 min after the last training session of the day, on chronic adaptations to training in elite athletes. Six short-track (ST) speed skaters completed four weeks of post-training HWB and four weeks of post-training passive recovery (PR) according to a randomized cross-over study. During HWB, participants sat in a jacuzzi (40 °C; 20 min). According to linear mixed models, maximal isometric strength of knee extensor muscles was significantly increased for training with HWB (p < 0.0001; d = 0.41) and a tendency (p = 0.0529) was observed concerning V ˙ O 2 m a x . No significant effect of training with PR or HWB was observed for several variables (p > 0.05), including aerobic peak power output, the decline rate of jump height during 1 min-continuous maximal countermovement jumps (i.e. anaerobic capacity index), and the force-velocity relationship. Regarding specific tasks on ice, a small effect of training was found on both half-lap time and total time during a 1.5-lap all-out exercise (p = 0.0487; d = 0.23 and p = 0.0332; d = 0.21, respectively) but no additional effect of HWB was observed. In summary, the regular HWB protocol used in this study can induce additional effects on maximal isometric strength without compromising aerobic and anaerobic adaptations or field performance in these athletes.

Validity of the Stryd Power Meter in Measuring Running Parameters at Submaximal Speeds.

This study assessed the Stryd running power meter validity at sub-maximal speeds (8 to 19 km/h). Six recreational runners performed an incremental indoor running test. Power output (PO), ground contact time (GCT) and leg spring stiffness (LSS) were compared to reference measures recorded by portable metabolic analyser, force platforms and motion capture system. A Bayesian framework was conducted for systems validity and comparisons. We observed strong and positive linear relationships between Stryd PO and oxygen consumption ( R 2 = 0.82 , B F 10 > 100 ), and between Stryd PO and external mechanical power ( R 2 = 0.88 , B F 10 > 100 ). Stryd power meter underestimated PO ( B F 10 > 100 ) whereas GCT and LSS values did not show any significant differences with the reference measures ( B F 10 = 0.008 , B F 10 = 0.007 , respectively). We conclude that the Stryd power meter provides valid measures of GCT and LSS but underestimates the absolute values of PO.

Increase in muscle power is associated with myofibrillar ATPase adaptations during resistance training.

NEW FINDINGS: What is the central question of this study? The aim of this study was to examine the effects of resistance training on gains in the external mechanical power output developed during climbing and myofibrillar ATPase activity in rats. What is the main finding and its importance? Using rapid flow quench experiments, we show that resistance training increases both the power output and the myofibrillar ATPase activity in the flexor digitorum profundus, biceps and deltoid muscles. Data fitting reveals that these functional ameliorations are explained by an increase in the rate constant of liberation of ATP hydrolysis products and contribute to performance gains. ABSTRACT: Skeletal muscle shows a remarkable plasticity that permits functional adaptations in response to different stimulations. To date, modifications of the proportions of myosin heavy chain (MHC) isoforms and increases in fibre size are considered to be the main factors providing sarcomeric plasticity in response to exercise training. In this study, we investigated the effects of a resistance training protocol on the myofibrillar ATPase (m-ATPase) cycle, muscle performance (power output) and MHC gene expression. For this purpose, 8-week-old Wistar Han rats were subjected to 4 weeks of resistance training, with five sessions per week. Muscle samples of flexor digitorum profundus (FDP), biceps and deltoid were collected and subjected to RT-qPCR analyses and assessment of m-ATPase activity with rapid flow quench apparatus. Training led to a significant increase in muscle mass, except for the biceps, and in total mechanical power output (+135.7%, P < 0.001). A shift towards an intermediate fibre type (i.e. MHC2x-to-MHC2a isoform transition) was also observed in biceps and FDP but not in the deltoid muscle. Importantly, rapid flow quench experiments revealed an enhancement of the m-ATPase activity during contraction at maximal velocity (kF ) in the three muscles, with a more marked effect in FDP (+242%, P < 0.001). Data fitting revealed that the rate constant of liberation of ATP hydrolysis products (k3 ) appears to be the main factor influencing the increase in m-ATPase activity. In conclusion, the data showed that, in addition to classically observed changes in MHC isoform content and fibre hypertrophy, m-ATPase activity is enhanced during resistance training and might contribute significantly to performance gains.

Recent Data on Cellular Component Turnover: Focus on Adaptations to Physical Exercise.

Significant progress has expanded our knowledge of the signaling pathways coordinating muscle protein turnover during various conditions including exercise. In this manuscript, the multiple mechanisms that govern the turnover of cellular components are reviewed, and their overall roles in adaptations to exercise training are discussed. Recent studies have highlighted the central role of the energy sensor (AMP)-activated protein kinase (AMPK), forkhead box class O subfamily protein (FOXO) transcription factors and the kinase mechanistic (or mammalian) target of rapamycin complex (MTOR) in the regulation of autophagy for organelle maintenance during exercise. A new cellular trafficking involving the lysosome was also revealed for full activation of MTOR and protein synthesis during recovery. Other emerging candidates have been found to be relevant in organelle turnover, especially Parkin and the mitochondrial E3 ubiquitin protein ligase (Mul1) pathways for mitochondrial turnover, and the glycerolipids diacylglycerol (DAG) for protein translation and FOXO regulation. Recent experiments with autophagy and mitophagy flux assessment have also provided important insights concerning mitochondrial turnover during ageing and chronic exercise. However, data in humans are often controversial and further investigations are needed to clarify the involvement of autophagy in exercise performed with additional stresses, such as hypoxia, and to understand the influence of exercise modality. Improving our knowledge of these pathways should help develop therapeutic ways to counteract muscle disorders in pathological conditions.

eIF3f depletion impedes mouse embryonic development, reduces adult skeletal muscle mass and amplifies muscle loss during disuse.

KEY POINTS: In muscular cells, eukaryotic initiation factor subunit f (eIF3f) activates protein synthesis by allowing physical interaction between mechanistic target of rapamycin complex 1 (MTORC1) and ribosomal protein S6 kinase 1 (S6K1), although its physiological role in animals is unknown. A knockout approach suggests that homozygous mice carrying a null mutation of the eIF3f gene fail to develop and consequently die at early embryonic stage, whereas heterozygous mice associated with a partial depletion of eIF3f gene grow normally and are phenotypically indistinguishable from wild-type mice. Heterozygous mice express reduced eIF3f mRNA and protein levels in skeletal muscles and show diminished muscle mass associated with a decrease in the protein synthesis rate and an inhibition of the MTORC1 pathway. During hindlimb immobilization, heterozygous eIF3f mice display an exacerbated immobilization-induced muscle atrophy associated with reduced protein synthesis. These results highlight the essential role of eIF3f during embryonic development and its involvement in muscular homeostasis via protein synthesis regulation. ABSTRACT: Eukaryotic translation initiation factor 3, subunit F (eIF3f), a component of eIF3 complex, plays an important role in protein synthesis regulation, although its physiological functions are unknown. We generated and analysed mice carrying a null mutation in the eIF3f gene. We showed that homozygous eIF3f knockout fail to develop and that eIF3f-/- embryos die at an early stage of development but after the pre-implantation stage. However, disrupting one eIF3f allele does not affect growth, viability and fertility of heterozygous mice but, instead, reduces eIF3f mRNA and protein levels in all tissues examined. Although heterozygous mice are phenotypically indistinguishable from wild-type mice, they present a diminished body weight and a lean mass reduction associated with normal body size. Interestingly, skeletal muscles are mainly affected and display an altered cell size without modification of fibre number. Skeletal muscles of heterozygous mice show a deficiency in polysome content, a decrease in protein synthesis rate and an inhibition of the mechanistic target of rapamycin (MTOR) pathway. We then studied the effects of hindlimb immobilization that mimic muscle disuse on heterozygous mice aiming to further explore the involvement of eIF3f in protein synthesis. We found that eIF3f partial depletion amplifies muscle atrophy compared to wild-type mice. Mass and cross-sectional area decreases were associated with reduced MTOR pathway activation and protein synthesis rate. Taken together, our data indicate that eIF3f is essential for mice embryonic development and controls adult skeletal muscle mass via protein synthesis regulation in a MTOR-dependent manner.

Systems model and individual simulations of training strategies in elite short-track speed skaters.

This study aimed to evaluate the effect of simulated training strategies on performance potential in elite short-track speed skaters. Training load and field-based criterion performances from fifteen athletes (10 males, 5 females) were collected over a 3-month training period and the relationship between training loads and performance was computed with a variable dose-response model using a genetic algorithm. Individual simulations of tapers preceded or not preceded by an overload training (OT) were assessed. We obtained a significant correlation between actual and modelled performances (R2 = 0.76 ± 0.07). Regarding model parameters, no significant difference was found between males and females but the time to recover performance tended to be lower in females. Simulations in which the taper parameters were free highlighted that an exponential or a step taper were the most effective for increasing performance compared to a linear taper (p < 0.05). Optimal exponential taper duration after OT was 10.7 ± 2.4d and the optimal load reduction was 75.9 ± 3.7%. OT intensity had the greatest influence on the predicted performance, followed by OT duration, taper decay, and to a lesser extent load reduction during taper and taper duration. Thus, a variable dose-response systems model allows the evaluation of different taper strategies and their potential effect on performance changes.

Modelling performance and skeletal muscle adaptations with exponential growth functions during resistance training.

System theory is classically applied to describe and to predict the effects of training load on performance. The classic models are structured by impulse-type transfer functions, nevertheless, most biological adaptations display exponential growth kinetics. The aim of this study was to propose an extension of the model structure taking into account the exponential nature of skeletal muscle adaptations by using a genetic algorithm. Thus, the conventional impulse-type model was applied in 15 resistance trained rodents and compared with exponential growth-type models. Even if we obtained a significant correlation between actual and modelled performances for all the models, our data indicated that an exponential model is associated with more suitable parameters values, especially the time constants that correspond to the positive response to training. Moreover, positive adaptations predicted with an exponential component showed a strong correlation with the main structural adaptations examined in skeletal muscles, i.e. hypertrophy (R2 = 0.87, 0.96 and 0.99, for type 1, 2A and 2X cross-sectional area fibers, respectively) and changes in fiber-type composition (R2 = 0.81 and 0.79, for type 1 and 2A fibers, respectively). Thus, an exponential model succeeds to describe both performance variations with relevant time constants and physiological adaptations that take place during resistance training.

AMP-activated protein kinase stabilizes FOXO3 in primary myotubes.

AMP-activated protein kinase (AMPK) is a critical enzyme in conditions of cellular energy deficit such as exercise, hypoxia or nutritional stress. AMPK is well known to regulate protein degradation pathways notably through FOXO-related axis. In this study, we investigated the implication of AMPK activation in FOXO3 expression and stability in skeletal muscle primary myotubes. First, time course and dose response studies revealed optimal AICAR treatment duration and dose in skeletal muscle cells. Then, experiments with cycloheximide treatment of primary myotubes highlighted that AICAR infusion extends FOXO3 protein half-life. Our results also showed that AICAR treatment or nutrient depletion increases FOXO3 expression in primary myotubes and the expression of the mitochondrial E3 ligase Mul1 involved in mitochondrial turnover (mitophagy). In AMPK KO cells, nutrient depletion failed to alter the level of some FOXO3-dependent atrophic genes, including LC3B, BNIP3, and the mitochondrial E3 ligase Mul1, but not the expression of other genes (i.e. FOXO1, Gabarapl1, MAFbx, MuRF1). In summary, our data highlight that AMPK stabilizes FOXO3 and suggest a role in the first initiation step of mitochondrial segregation in muscle cells.

Early structural and functional signature of 3-day human skeletal muscle disuse using the dry immersion model.

KEY POINTS: Our study contributes to the characterization of muscle loss and weakness processes induced by a sedentary life style, chronic hypoactivity, clinical bed rest, immobilization and microgravity. This study, by bringing together integrated and cellular evaluation of muscle structure and function, identifies the early functional markers and biomarkers of muscle deconditioning. Three days of muscle disuse in healthy adult subjects is sufficient to significantly decrease muscle mass, tone and force, and to induce changes in function relating to a weakness in aerobic metabolism and muscle fibre denervation. The outcomes of this study should be considered in the development of an early muscle loss prevention programme and/or the development of pre-conditioning programmes required before clinical bed rest, immobilization and spaceflight travel. ABSTRACT: Microgravity and hypoactivity are associated with skeletal muscle deconditioning. The decrease of muscle mass follows an exponential decay, with major changes in the first days. The purpose of the study was to dissect out the effects of a short-term 3-day dry immersion (DI) on human quadriceps muscle function and structure. The DI model, by suppressing all support zones, accurately reproduces the effects of microgravity. Twelve healthy volunteers (32 ± 5 years) completed 3 days of DI. Muscle function was investigated through maximal voluntary contraction (MVC) tests and muscle viscoelasticity. Structural experiments were performed using MRI analysis and invasive experiments on muscle fibres. Our results indicated a significant 9.1% decrease of the normalized MVC constant (P = 0.048). Contraction and relaxation modelization kinetics reported modifications related to torque generation (kACT  = -29%; P = 0.014) and to the relaxation phase (kREL  = +34%; P = 0.040) after 3 days of DI. Muscle viscoelasticity was also altered. From day one, rectus femoris stiffness and tone decreased by, respectively, 7.3% (P = 0.002) and 10.2% (P = 0.002), and rectus femoris elasticity decreased by 31.5% (P = 0.004) after 3 days of DI. At the cellular level, 3 days of DI translated into a significant atrophy of type I muscle fibres (-10.6 ± 12.1%, P = 0.027) and an increased proportion of hybrid, type I/IIX fibre co-expression. Finally, we report an increase (6-fold; P = 0.002) in NCAM+ muscle fibres, showing an early denervation process. This study is the first to report experiments performed in Europe investigating human short-term DI-induced muscle adaptations, and contributes to deciphering the early changes and biomarkers of skeletal muscle deconditioning.

Muscle Regeneration with Intermuscular Adipose Tissue (IMAT) Accumulation Is Modulated by Mechanical Constraints.

Sports trauma are able to induce muscle injury with fibrosis and accumulation of intermuscular adipose tissue (IMAT), which affect muscle function. This study was designed to investigate whether hypoactivity would influence IMAT accumulation in regenerating mouse skeletal muscle using the glycerol model of muscle regeneration. The animals were immediately hindlimb unloaded for 21 days after glycerol injection into the tibialis anterior (TA) muscle. Muscle fiber and adipocyte cross-sectional area (CSA) and IMAT accumulation were determined by histomorphometric analysis. Adipogenesis during regenerative processes was examined using RT-qPCR and Western blot quantification. Twenty-one days of hindlimb unloading resulted in decreases of 38% and 50.6% in the muscle weight/body weight ratio and CSA, respectively, in soleus muscle. Glycerol injection into TA induced IMAT accumulation, reaching 3% of control normal-loading muscle area. This IMAT accumulation was largely inhibited in unloading conditions (0.09%) and concomitant with a marked reduction in perilipin and FABP4 protein content, two key markers of mature adipocytes. Induction of PPARγ and C/EBPα mRNA, two markers of adipogenesis, was also decreased. Furthermore, the protein expression of PDGFRα, a cell surface marker of fibro/adipogenic progenitors, was much lower in regenerating TA from the unloaded group. Exposure of regenerating muscle to hypoactivity severely reduces IMAT development and accumulation. These results provide new insight into the mechanisms regulating IMAT development in skeletal muscle and highlight the importance of taking into account the level of mechanical constraint imposed on skeletal muscle during the regeneration processes.