ABSTRACT
Development of lanthanide-based luminescent "switch-on" systems via azide-alkyne [3+2] cycloaddition is described. We used these for non-specific protein labeling and as tags for specific and selective activity-based protein labeling.
Subject(s)
Coordination Complexes/chemistry , Lanthanoid Series Elements/chemistry , Proteins/chemistry , Alkynes/chemistry , Animals , Azides/chemistry , Cattle , Click Chemistry , Cycloaddition Reaction , Papain/chemistry , Papain/metabolism , Proteins/metabolism , Serum Albumin, Bovine/chemistry , Serum Albumin, Bovine/metabolism , Spectrometry, FluorescenceABSTRACT
The facile synthesis of functionalized luminescent nanoparticles from LnL3 lanthanide complexes is described. The luminescence properties of the lanthanide chelates and of the corresponding nanohybrids are reported and compared. For a further application in bioimaging, the cytotoxicity of the nano-objects was investigated.
ABSTRACT
New trisdipicolinic acid-lanthanide complexes are reported as phasing agents for X-ray crystallography of proteins. It is demonstrated that CuAAC modifications allow protein co-crystallization with low concentration of lanthanide complexes leading to an accurate structure determination.
Subject(s)
Europium/chemistry , Muramidase/chemistry , Organometallic Compounds/chemistry , Picolinic Acids/chemistry , Plant Proteins/chemistry , Crystallography, X-Ray , Models, Molecular , Molecular Structure , Muramidase/metabolism , Organometallic Compounds/chemical synthesisABSTRACT
4-Triazolyl-dipicolinic acids, prepared efficiently by CuAAC, proved to act as efficient antennae for optical lanthanide probes containing Eu(3+) or Tb(3+), under linear and non linear excitations (one, two and three photons provided by laser induced luminescence). The versatility offered by the substitution of the triazole subunit is, in addition, useful for covalent linkage to slide glass providing luminescent films.
