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1.
Sci Adv ; 8(39): eabp8416, 2022 Sep 30.
Article in English | MEDLINE | ID: mdl-36179021

ABSTRACT

Cell migration is essential to living organisms and deregulated in cancer. Single cell's migration ranges from traction-dependent mesenchymal motility to contractility-driven propulsive amoeboid locomotion, but collective cell migration has only been described as a focal adhesion-dependent and traction-dependent process. Here, we show that cancer cell clusters, from patients and cell lines, migrate without focal adhesions when confined into nonadhesive microfabricated channels. Clusters coordinate and behave like giant super cells, mobilizing their actomyosin contractility at the rear to power their migration. This polarized cortex does not sustain persistent retrograde flows, of cells or actin, like in the other modes of migration but rather harnesses fluctuating cell deformations, or jiggling. Theoretical physical modeling shows this is sufficient to create a gradient of friction forces and trigger directed cluster motion. This collective amoeboid mode of migration could foster metastatic spread by enabling cells to cross a wide spectrum of environments.

2.
J Cell Sci ; 135(14)2022 07 15.
Article in English | MEDLINE | ID: mdl-35703098

ABSTRACT

The metastatic progression of cancer remains a major issue in patient treatment. However, the molecular and cellular mechanisms underlying this process remain unclear. Here, we use primary explants and organoids from patients harboring mucinous colorectal carcinoma (MUC CRC), a poor-prognosis histological form of digestive cancer, to study the architecture, invasive behavior and chemoresistance of tumor cell intermediates. We report that these tumors maintain a robust apico-basolateral polarity as they spread in the peritumoral stroma or organotypic collagen-I gels. We identified two distinct topologies - MUC CRCs either display a conventional 'apical-in' polarity or, more frequently, harbor an inverted 'apical-out' topology. Transcriptomic analyses combined with interference experiments on organoids showed that TGFß and focal adhesion signaling pathways are the main drivers of polarity orientation. Finally, we show that the apical-out topology is associated with increased resistance to chemotherapeutic treatments in organoids and decreased patient survival in the clinic. Thus, studies on patient-derived organoids have the potential to bridge histological, cellular and molecular analyses to decrypt onco-morphogenic programs and stratify cancer patients. This article has an associated First Person interview with the first author of the paper.


Subject(s)
Colorectal Neoplasms , Organoids , Cell Adhesion , Colorectal Neoplasms/genetics , Colorectal Neoplasms/metabolism , Humans , Signal Transduction , Transforming Growth Factor beta/metabolism
3.
Nat Cell Biol ; 20(3): 296-306, 2018 03.
Article in English | MEDLINE | ID: mdl-29403038

ABSTRACT

Metastases account for 90% of cancer-related deaths; thus, it is vital to understand the biology of tumour dissemination. Here, we collected and monitored >50 patient specimens ex vivo to investigate the cell biology of colorectal cancer (CRC) metastatic spread to the peritoneum. This reveals an unpredicted mode of dissemination. Large clusters of cancer epithelial cells displaying a robust outward apical pole, which we termed tumour spheres with inverted polarity (TSIPs), were observed throughout the process of dissemination. TSIPs form and propagate through the collective apical budding of hypermethylated CRCs downstream of canonical and non-canonical transforming growth factor-ß signalling. TSIPs maintain their apical-out topology and use actomyosin contractility to collectively invade three-dimensional extracellular matrices. TSIPs invade paired patient peritoneum explants, initiate metastases in mice xenograft models and correlate with adverse patient prognosis. Thus, despite their epithelial architecture and inverted topology TSIPs seem to drive the metastatic spread of hypermethylated CRCs.


Subject(s)
Biomarkers, Tumor/genetics , Cell Movement , Cell Polarity , Colorectal Neoplasms/genetics , Colorectal Neoplasms/pathology , DNA Methylation , Epithelial Cells/pathology , Peritoneal Neoplasms/genetics , Peritoneal Neoplasms/secondary , Animals , Biomarkers, Tumor/metabolism , Caco-2 Cells , Colorectal Neoplasms/metabolism , Epithelial Cells/metabolism , Genetic Predisposition to Disease , Humans , Mice, Inbred NOD , Mice, SCID , Neoplasm Invasiveness , Peritoneal Neoplasms/metabolism , Phenotype , Prospective Studies , Signal Transduction , Time Factors , Transforming Growth Factor beta/metabolism , Tumor Cells, Cultured , Tumor Microenvironment
4.
PLoS One ; 9(10): e109256, 2014.
Article in English | MEDLINE | ID: mdl-25275312

ABSTRACT

IL-32γ is a multifunctional cytokine involved in various inflammatory and auto-immune diseases in which neutrophils can affect the evolution of these diseases. To persist at inflammatory sites, neutrophils require inhibition of their rapid and constitutive apoptosis, an inhibitory effect that phlogogenic cytokines support. To date, the effects of IL-32γ on neutrophils remain unknown. We demonstrate that IL-32γ delays, in a dose-dependent manner, the spontaneous apoptosis of human blood neutrophils by activating mainly p38 MAPK through rapid p38 phosphorylation. PI3-K and ERK1/2 MAPK are also involved, but to a lesser extent. Most of cytokines that induce retardation of neutrophil apoptosis activate the expression of MCL-1 at both mRNA and protein levels. IL-32γ added to human blood neutrophils in vitro is associated with sustained levels of MCL-1 protein. This effect in neutrophils corresponds to a decrease of MCL-1 protein degradation without any effect on MCL-1 mRNA levels. The sustained levels of MCL-1 induced by IL-32γ are only abrogated by the p38ß MAPK inhibitor SB202190. Additionally, IL-32γ induces a reduction in caspase 3 activity in neutrophils. In conclusion, IL-32γ affects human blood neutrophils in vitro by increasing their survival, suggesting that this cytokine could have profound effects on the deleterious functions of neutrophils in several diseases.


Subject(s)
Apoptosis , Interleukins/immunology , MAP Kinase Signaling System , Myeloid Cell Leukemia Sequence 1 Protein/immunology , Neutrophils/cytology , p38 Mitogen-Activated Protein Kinases/immunology , Cells, Cultured , Humans , Neutrophils/immunology , Phosphatidylinositol 3-Kinases/immunology
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