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1.
Mol Genet Genomics ; 270(4): 347-61, 2003 Dec.
Article in English | MEDLINE | ID: mdl-14598165

ABSTRACT

Apyrases have been suggested to play important roles in plant nutrition, photomorphogenesis, and nodulation. To help trace the evolution of these genes in the legumes--and possibly, the acquisition of new functions for nodulation--apyrase-containing BACs were sequenced from three legume genomes. Genomic sequences from Medicago truncatula, Glycine max and Lotus japonicus were compared to one another and to corresponding regions in Arabidopsis thaliana. A phylogenetic analysis of apyrase homologs from these regions and sequences from other legume species, as well as other plant families, identified a potentially legume-specific clade that contains a well-characterized soybean ( G. soja) apyrase, Gs52, as well as homologs from Dolichos, Lotus, Medicago and Pisum. Sister clades contain homologs from members of Brassicaceae, Solanaceae, Poaceae and Fabaceae. Comparisons of rates of change at synonymous and nonsynonymous sites in the Gs52 and sister clades show rapid evolution in the potentially legume-specific Gs52 clade. The genomic organization of the apyrase-containing BACs shows evidence of gene duplication, genomic rearrangement, and gene conversion among Gs52 homologs. Taken together, these results suggest a scenario of local apyrase gene duplication in an ancestor of the legumes, followed by functional diversification and increased rates of change in the new genes, and further duplications in the Galegae (which include the genera Medicago and Pisum). The study also provides a detailed comparison of genomic regions between two model genomes which are now being sequenced ( M. truncatulaand L. japonicus), and a genome from an economically important legume species ( G. max).


Subject(s)
Apyrase/genetics , Evolution, Molecular , Fabaceae/genetics , Synteny , Chromosomes, Artificial, Bacterial , Fabaceae/enzymology , Gene Duplication , Genetic Variation , Genome , Models, Genetic , Phylogeny , Sequence Homology
2.
Mol Genet Genomics ; 270(1): 34-45, 2003 Oct.
Article in English | MEDLINE | ID: mdl-12928866

ABSTRACT

Arachis hypogaea L., commonly known as the peanut or groundnut, is an important and widespread food legume. Because the crop has a narrow genetic base, genetic diversity in A. hypogaea is low and it lacks sources of resistance to many pests and diseases. In contrast, wild diploid Arachis species are genetically diverse and are rich sources of disease resistance genes. The majority of known plant disease resistance genes encode proteins with a nucleotide binding site domain (NBS). In this study, degenerate PCR primers designed to bind to DNA regions encoding conserved motifs within this domain were used to amplify NBS-encoding regions from Arachis spp. The Arachis spp. used were A. hypogaea var. Tatu and wild species that are known to be sources of disease resistance: A. cardenasii, A. duranensis, A. stenosperma and A. simpsonii. A total of 78 complete NBS-encoding regions were isolated, of which 63 had uninterrupted ORFs. Phylogenetic analysis of the Arachis NBS sequences derived in this study and other NBS sequences from Arabidopsis thaliana, Medicago trunculata, Glycine max, Lotus japonicus and Phaseolus vulgaris that are available in public databases This analysis indicates that most Arachis NBS sequences fall within legume-specific clades, some of which appear to have undergone extensive copy number expansions in the legumes. In addition, NBS motifs from A. thaliana and legumes were characterized. Differences in the TIR and non-TIR motifs were identified. The likely effect of these differences on the amplification of NBS-encoding sequences by PCR is discussed.


Subject(s)
Arachis/classification , Arachis/genetics , Genes, Plant , Phylogeny , Arabidopsis/classification , Arabidopsis/genetics , Base Sequence , DNA Primers , Immunity, Innate , Molecular Sequence Data , Plant Diseases/genetics , Polymerase Chain Reaction
3.
Nurs Health Care Perspect ; 22(4): 194-6, 2001.
Article in English | MEDLINE | ID: mdl-16379267

ABSTRACT

This article describes a course designed to prepare RN-BSN students to assimilate relevant research findings into clinical practice. The course was developed as faculty accepted the challenge of providing didactic content completely online. Guided by two faculty members, two groups of students developed research proposals to respond to community needs identified by a local hospital and a nursing center.


Subject(s)
Community Health Services , Community-Institutional Relations , Education, Nursing, Baccalaureate , Nursing Research/education , Education, Distance , Humans , Internet , Needs Assessment , Texas
4.
J Insur Med ; 33(4): 360-2, 2001.
Article in English | MEDLINE | ID: mdl-11877919

ABSTRACT

Ta and T1 transitional cell urinary bladder tumors are prone to recurrence in a large percentage of cases, but they uncommonly progress to invasive tumors. On the other hand, Tis bladder tumors progress to invasive tumors in a majority of cases. Even though all of these tumors are found in the superficial layers of the bladder wall, their natural histories are significantly different. In addition, new terminology has been developed for these lesions.


Subject(s)
Carcinoma, Transitional Cell/pathology , Terminology as Topic , Urinary Bladder Neoplasms/pathology , Carcinoma, Transitional Cell/classification , Humans , Neoplasm Staging , United States , Urinary Bladder Neoplasms/classification
6.
Cancer ; 48(3): 774-8, 1981 Aug 01.
Article in English | MEDLINE | ID: mdl-6166364

ABSTRACT

From September 1974 through November 1976, 13 adolescent patients with mucin-producing colorectal adenocarcinoma were treated at a Memphis, Tennessee, pediatric oncology center. Ten of these children were from rural areas of the Mississippi Delta, areas of high pesticide use. None of them had a family history of colorectal cancer, familial polyposis, or ulcerative colitis. Levels of pesticide residues, however, were not generally higher in blood samples from patients and their families than from controls.


Subject(s)
Adenocarcinoma/blood , Colonic Neoplasms/blood , Pesticides/blood , Adenocarcinoma/etiology , Adolescent , Adult , Aged , Agriculture , Colonic Neoplasms/etiology , DDT/blood , Dieldrin/blood , Female , Heptachlor Epoxide/blood , Hexachlorocyclohexane/blood , Humans , Male , Middle Aged , Mucins/metabolism , Rural Population
8.
Am J Epidemiol ; 107(6): 529-37, 1978 Jun.
Article in English | MEDLINE | ID: mdl-78669

ABSTRACT

From March 1974 through July 1975, 76 (56%) of 133 persons who had worked at a pesticide plant that produced Kepone, a chlorinated hydrocarbon insecticide, contracted a previously unrecognized clinical illness characterized by nervousness, tremor, weight loss, opsoclonus, pleuritic and joint pain, and oligospermia. Illness incidence rates for production workers (64%) were significantly higher than for nonproduction personnel (16%). The mean blood Kepone level for workers with illness was 2.53 ppm and for those without disease 0.60 ppm (p less than 0.001). Blood Kepone levels in current workers (mean, 3.12 ppm) were higher than those in former employees (1.22 ppm). Blood Kepone levels for workers in nearby businesses and for residents of a community within 1.6 km of the plant ranged from undetectable to 32.5 ppb. Illness attributable to Kepone was found in two wives of Kepone workers; there was no apparent association between frequency of symptoms and proximity to the plant in the survey of the community population.


PIP: From March 1974-July 1975, 76 (57%) of 133 persons who had worked at a pesticide plant that produced Kepone, a chlorinated hydrocarbon insecticide, contracted a previously unrecognized clinical illness characterized by nervousness, tremor, weight loss, opsoclonus, pleuritic and joint pain, and oligospermia. Illness incidence rates for production workers (64%) were significantly higher than for nonproduction personnel (16%). The mean blood Kepone level for workers with illness was 2.53 ppm and for those without disease 0.60 ppm (p0.001). Blood Kepone levels in current workers (mean, 3.12 ppm) were higher than those in former employees (1.22 ppm). Blood Kepone levels for workers in nearby businesses and for residents of a community within 1.6 km of the plant ranged from undetectable to 32.5 ppb. Illness attributable to Kepone was found in wives of 2 Kepone workers; there was no apparent association between frequency of symptoms and proximity to the plant in the survey of the community population.


Subject(s)
Chemical Industry , Chlordecone/poisoning , Insecticides/poisoning , Occupational Diseases/epidemiology , Chlordecone/blood , Chlordecone/chemical synthesis , Environmental Exposure , Female , Humans , Insecticides/chemical synthesis , Male , Occupational Diseases/diagnosis , Pesticides/chemical synthesis , Poisoning/diagnosis , Virginia
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