ABSTRACT
Resumen: La Espectroscopia Fotoacústica (EFA), ha destacado de entre las técnicas fototérmicas por su versatilidad para la caracterización de diversos tipos de muestras, incluyendo las de tipo biológicas. En este estudio, se compararon y analizaron mediante EFA, las diferencias entre los espectros de absorción ópticos de sangre de ratas macho Fisher en tres casos, control (ratas sanas), con daño hepático, y con daño hepático tratadas con Curcuma longa. Los resultados experimentales demostraron diferencias en los espectros de absorción ópticos de cada caso analizado, por lo que EFA se propondría como una técnica complementaria, no convencional para el estudio del efecto de la Curcuma longa en daño hepático inducido en modelo animal.
Abstract: Photoacoustic Spectroscopy (PAS) stand outs among Photothermal techniques due to its versatility for the characterization of different types of materials, including biological samples. In this study, were compared and analyzed by PAS, the differences between the optical absorption spectra of blood of male Fisher rats, in three cases, control (healthy rats), liver damage and liver damage treated with Curcuma longa. The experimental results show differences in the optical absorption spectra of each analysed case, therefore PAS would be proposed as a non conventional complementary technique, to study the effect of Curcuma longa in induced liver damage for an animal model.
ABSTRACT
Our objective was to determine if the phycobiliproteins of Arthrospira (Spirulina) maxima protect renal cells against mercury-caused oxidative stress and cellular damage in the kidney. We used 40 male mice that were assigned into eight groups: (1) a control group that received 100mM phosphate buffer (PB) ig and 0.9% saline ip, (2) PB+HgCl(2) (5mg/kg ip), (3) PB plus phycobiliproteins (100mg/kg ig), (4) PB plus C-phycocyanin (100mg/kg ig), and four groups receiving HgCl(2)+phycobiliproteins or C-phycocyanin (50, and 100mg/kg ig). The left kidneys were used to determine lipid peroxidation, quantification of reactive oxygen species, and reduced glutathione and oxidised content. The right kidneys were processed for histology. The HgCl(2) caused oxidative stress and cellular damage. All doses of phycobiliproteins or C-phycocyanin prevented enhancement of oxidative markers and they protected against HgCl(2)-caused cellular damage.
Subject(s)
Bacterial Proteins/administration & dosage , Cyanobacteria/chemistry , Kidney Diseases/drug therapy , Kidney/drug effects , Mercuric Chloride/toxicity , Oxidative Stress/drug effects , Phycobiliproteins/administration & dosage , Phycocyanin/administration & dosage , Protective Agents/administration & dosage , Animals , Humans , Kidney/metabolism , Kidney Diseases/chemically induced , Kidney Diseases/metabolism , Male , MiceABSTRACT
Palmitone is a secondary metabolite of polyketide origin extracted from leaves of Annona diversifolia Saff. (Annonaceae). We found that palmitone possesses anticonvulsant properties against penicillin-, 4-AP-, and pentylenetetrazole (PTZ)-caused seizure in adult animals. Some convulsants as PTZ cause neuronal damage in different brain regions such as the CA3 hippocampal region. Our objective was to evaluate if palmitone protects against PTZ-caused seizures and hippocampal neuronal damage in prepubertal rats. We used 32 prepubertal Wistar rats (30-35 days old) divided into four groups of 8 animals; group I was the control group, group II received a single PTZ dose of 50mg/kg ip, group III received a single palmitone dose of 50mg/kg ip, and group IV received a palmitone dose of 50mg/kg ip plus a PTZ dose of 50mg/kg ip. Ten days after administration, the animals were killed using pentobarbital anesthesia (35 mg/kg). The brains were removed and were embedded in paraffin. Coronal cuts of 7 microm were obtained from -2.8 to -3.3 from Bregma. Each section was stained with cresyl violet-eosin. We evaluated the number of normal and abnormal neurons in the CA3 hippocampal region in a 10,000 microm(2) section. It was observed that palmitone did not prevent the PTZ-caused seizure but palmitone prevents the PTZ-caused neuronal damage in the CA3 hippocampal region.