ABSTRACT
With the development of technology, people are increasingly under the exposure of electromagnetic fields. Individuals with chronic diseases such as diabetes are now long-term exposed to Radio Frequency-RF radiation and extremely low frequency (ELF) magnetic fields (MFs). The purpose of this present study is to investigate oxidative effects and antioxidant parameters of ELF MFs and RF radiation on testis tissue in diabetic and healthy rats. Wistar male rats were divided into 10 groups. Intraperitoneal single dose STZ (65 mg/kg) dissolved in citrate buffer (0.1M (pH 4.5)) was injected to diabetes groups. ELF MFs and RF radiation were used as an electromagnetic exposure for 20 min/day, 5 days/week for one month. Testis tissue oxidant malondialdehyde (MDA), and antioxidants glutathione (GSH), and total nitric oxide (NOx) levels were determined. The results of ANOVA and Mann-Whitney tests were compared; p < 0.05 was considered significant. ELF and RF radiation resulted in an increase in testicular tissue MDA and NOX levels (p < 0.05), and caused a decrease in GSH levels (p < 0.05) in both healthy and diabetic rats, yet more distinctively in diabetic rats. The most pronounced effect was recorded in D-RF + ELF group (p < 0.005). Both radiation practices increased the oxidative stress in testis tissue while causing a decrease in antioxidant level which was more distinctive in diabetic rats (Tab. 1, Fig. 3, Ref. 30).
Subject(s)
Diabetes Mellitus, Experimental/metabolism , Electromagnetic Fields/adverse effects , Lipid Peroxidation/radiation effects , Oxidative Stress/radiation effects , Testis/radiation effects , Animals , Antioxidants/pharmacology , Glutathione/radiation effects , Magnetic Fields , Male , Oxidative Stress/drug effects , Rats , Rats, WistarABSTRACT
This study was planned so as to evaluate whether magnetic field exposure has any significant effect on the pentylenetetrazol (PTZ)-induced seizures. Mice were exposed to 50 Hz, 2 G (0.2 mT) magnetic field in glass cages for 1 h. Sham exposure was produced by turning off the current while the animals were in the same exposure volume. Then, PTZ was administered intraperitoneally (i.p.) at a dose of 60 mg/kg and the animals were observed for 30 min. Subsequently, the latency to seizure onset, total seizure duration, the number of seizure episodes and mortality were recorded for each subject. There was no evidence for a significant effect of the 50 Hz magnetic field on the mean number of PTZ induced seizures, seizure latency, total seizure duration and mortality (P>0.05). As a conclusion the present study failed to provide any further support for a therapeutic potential of magnetic field.
Subject(s)
Convulsants , Electromagnetic Fields , Epilepsy, Generalized , Pentylenetetrazole , Animals , Epilepsy, Generalized/chemically induced , Epilepsy, Generalized/mortality , Epilepsy, Generalized/prevention & control , Female , Mice , Pilot ProjectsABSTRACT
The effect of direct micro currents on collagen synthesis in wound healing on the 3rd day of wounding has been examined in rabbit skin. Two circular skin wounds were formed bilaterally on the gluteal region and current was applied to one of the wounds and the other was taken as the control with placebo electrode on it, the current intensities being 200 microA and 400 microA and application time 72 hrs with 8 hr/day. The hydroxyproline content was found to be increased in wound tissue at the end of 72 hrs of applying current which indicated that collagen synthesis is promoted by electric current.
