Analysis of translucency parameter and fluorescence intensity of 5 resin composite systems.

The natural outcome of dental composite restorations highly depends on the translucency of the enamel layer and fluorescence. This study aimed to evaluate the Translucency Parameter (TP) and Fluorescence Intensity (FI) of five different resin composite systems. Seven discs of each composite brand were prepared in a circular increasing thickness. For TP, a spectrophotometer measured the samples' colors. The color difference within the white/black backgrounds obtained the translucency parameter. For FI, samples were exposed to UV light, and ten photographs per group were taken. Each specimen was analyzed digitally. A mixed model analysis to a 95% confidence level analyzed groups differences. Higher values of TP were observed for ED and EL, followed by FZ. The lowest values were observed for EO and FO. FI values descending order was EL>FO>EO>ED>FZ. The composition of fillers and organic matrix influenced the behavior of fluorescence and translucency of resin composites. Key words:Resin composite, fluorescence, color, translucency parameter.

Maximizing the immunological output of the cervicovaginal explant model.

In the field of sexually transmitted infections (STI), the cervicovaginal explant (CVEx) model, not only provides the opportunity to study the different immunological arms present in these tissues under steady state conditions, but also their response against ex vivo infection with relevant pathogens. The methodology associated to the establishment of the HIV infection model in the cervicovaginal tissue was described in detail by Grivel et al. earlier (Grivel and Margolis, 2009). With this model as a foundation, we illustrate different approaches to obtain a large number of immunological readouts from a single piece of tissue, thus maximizing the immunological output obtained. Additionally, we discuss several ideas to study some of the immunological subsets present in this mucosal tissue by enriching them with the addition of distinct chemokines or specifically inducing their activation. Importantly, most of the methodology and concepts proposed here can be applied to study the immune subsets resident in other tissues. In the field of mucosal immunology, the possibility of studying resident immune subsets from tissue explants offers a great opportunity to understand the real players against invading pathogens and localized pathologies. Furthermore, this model allows for addressing the therapeutic benefit of modulating the activity of certain molecules and immune subsets against invading pathogens, which may infer their contribution to pathogen control and direct novel therapeutic interventions.

Adhesion Molecules Associated with Female Genital Tract Infection.

Efforts to develop vaccines that can elicit mucosal immune responses in the female genital tract against sexually transmitted infections have been hampered by an inability to measure immune responses in these tissues. The differential expression of adhesion molecules is known to confer site-dependent homing of circulating effector T cells to mucosal tissues. Specific homing molecules have been defined that can be measured in blood as surrogate markers of local immunity (e.g. α4ß7 for gut). Here we analyzed the expression pattern of adhesion molecules by circulating effector T cells following mucosal infection of the female genital tract in mice and during a symptomatic episode of vaginosis in women. While CCR2, CCR5, CXCR6 and CD11c were preferentially expressed in a mouse model of Chlamydia infection, only CCR5 and CD11c were clearly expressed by effector T cells during bacterial vaginosis in women. Other homing molecules previously suggested as required for homing to the genital mucosa such as α4ß1 and α4ß7 were also differentially expressed in these patients. However, CD11c expression, an integrin chain rarely analyzed in the context of T cell immunity, was the most consistently elevated in all activated effector CD8+ T cell subsets analyzed. This molecule was also induced after systemic infection in mice, suggesting that CD11c is not exclusive of genital tract infection. Still, its increase in response to genital tract disorders may represent a novel surrogate marker of mucosal immunity in women, and warrants further exploration for diagnostic and therapeutic purposes.

Expression of CD11c Is Associated with Unconventional Activated T Cell Subsets with High Migratory Potential.

CD11c is an α integrin classically employed to define myeloid dendritic cells. Although there is little information about CD11c expression on human T cells, mouse models have shown an association of CD11c expression with functionally relevant T cell subsets. In the context of genital tract infection, we have previously observed increased expression of CD11c in circulating T cells from mice and women. Microarray analyses of activated effector T cells expressing CD11c derived from naïve mice demonstrated enrichment for natural killer (NK) associated genes. Here we find that murine CD11c+ T cells analyzed by flow cytometry display markers associated with non-conventional T cell subsets, including γδ T cells and invariant natural killer T (iNKT) cells. However, in women, only γδ T cells and CD8+ T cells were enriched within the CD11c fraction of blood and cervical tissue. These CD11c+ cells were highly activated and had greater interferon (IFN)-γ secretory capacity than CD11c- T cells. Furthermore, circulating CD11c+ T cells were associated with the expression of multiple adhesion molecules in women, suggesting that these cells have high tissue homing potential. These data suggest that CD11c expression distinguishes a population of circulating T cells during bacterial infection with innate capacity and mucosal homing potential.