ABSTRACT
Rotavirus was detected in 52% of 2328 stool specimens collected from children with acute gastroenteritis admitted to three sentinel hospitals in Mainland China from January 2006 to December 2007. G3P[8] (42%) was the most common strain. Despite being common globally, only 18 (2%) G9-positive samples were identified. The VP7, VP4, VP6, and NSP4 genes were sequences for 13 of the G9 strains with G9P[8] being most common and showing the same origin as G9 strains reported in other countries. One G9P[6] strain was possibly derived by reassortment between earlier Chinese G9 strains and more recent local P[6] strains.
Subject(s)
Diarrhea/epidemiology , Gastroenteritis/epidemiology , Rotavirus Infections/epidemiology , Rotavirus/genetics , Child, Preschool , China/epidemiology , Diarrhea/virology , Gastroenteritis/virology , Genes, Viral , Genotype , Humans , Infant , Molecular Epidemiology , Prevalence , RNA, Viral/genetics , Sentinel Surveillance , Sequence Analysis, RNAABSTRACT
OBJECTIVE: Building a method which can examines virus pathogenic in gastroenteritis excrement specimen. METHODS: Choosing six positive specimens which tested in our laboratory, include adenovirus, calicivirus, rotavirus, bocavirus, astrovirus and enterovirus. Through sequence-independent single primer amplification(SISPA) constructs a gene bank. Looks up the viral gene fragment in gene bank. RESULTS: Obtaining corresponding viral acid sequence in six specimens. CONCLUSION: This research can examine enterovirus and the virus which cause diarrhea, It make a foundation for further studies the viral cause of disease which the examination not yet discovered at present.
