Development of Single Nucleotide Polymorphism and Association Analysis with Growth Traits for Black Porgy (Acanthopagrus schlegelii).

Black porgy is an important marine aquaculture fish species whose production is at the fifth position in all kinds of marine-cultured fishes in China. In this study, Illumina high-throughput sequencing technology was used to sequence the total RNA of black porgy. Sixty-one candidate SNPs (Single Nucleotide Polymorphism) were screened out and genotyped through GATK4 (Genome Analysis ToolKit) software and MALDI-TOF MS (Matrix-Assisted Laser Desorption/ Ionization Time of Flight Mass Spectrometry). The experimental results showed that a total of sixty SNPs were successfully genotyped, with a success rate of 98.36%. The results of principal component analysis and correlation analysis of growth traits showed that body weight was the first principal component, with a cumulative contribution rate of 74%. There were significant correlations (p < 0.05) or extremely significant correlations (p < 0.01) between different growth traits. The results of genetic parameter analysis and association analysis showed that scaffold12-12716321, scaffold13-4787950, scaffold2-13687576 and scaffold290-11890 were four SNPs that met the requirement of polymorphic information content and conformed to the Hardy-Weinberg equilibrium. There were significant differences between their genotype and the phenotype of growth traits. The four SNP molecular markers developed in this research will lay a foundation for further exploration of molecular markers related to the growth traits of black porgy and will provide a scientific reference for the further study of its growth mechanisms. At the same time, these molecular markers can be applied to the production practices of black porgy, so as to realize selective breeding at the molecular level and speed up the breeding process.

Study on Behavior and Bearing Capacity Computation Method of Shallow Rock-Socketed Short Piles Based on the Self-Balanced Loading Test.

The self-balanced loading test is a state-of-art pile testing method, but its suitability to pile bearing capacity determination in transformer substation engineering in mountainous and hilly areas is not yet clear. In this study, a two-dimensional axisymmetric numerical model is established by the PLAXIS software to simulate the behavior and bearing mechanism of shallow rock-socketed short piles based on the self-balanced loading test. The model is first validated by simulating the field tests of two adjacent piles under self-balanced loading. Then the influence factors of the load-displacement curves of piles are analyzed. Thereafter, the mechanical mechanism of the self-balanced loading tests is simulated and compared with the conventional static loading tests. It is observed that the rock modulus, rock-socketed depth of piles, and burial depth of the Osterberg Cell affect the load-displacement significantly, but the cohesion of the rocks affects little. Moreover, compared with the conventional static loading tests, the shear stress of the pile-soil interface distributes less uniformly under self-balanced loading conditions. On this basis, a bearing capacity computation method of shallow rock-socketed short piles based on the self-balanced loading test is proposed.