Relationship of Earthquake Exposure, Locus of Control, Coping Strategy, and Posttraumatic Stress Disorder Among Adolescent Survivors.

A cross-sectional study was performed to examine the incidence of posttraumatic stress disorder (PTSD) among adolescent survivors 2 years after the 2017 Jiuzhaigou earthquake, and to identify risk factors and their interaction effects. A total of 4,131 adolescents were recruited. PTSD was assessed using the PTSD Checklist-Civilian Version, and locus of control (LOC) and coping strategy were assessed using the Internality, Powerful Others and Chance Scale and Coping Styles Scale, respectively. The prevalence of PTSD was 5% and the relationship between external LOC and PTSD and negative coping and PTSD seemed moderated by environmental factors. These factors should be considered along with external LOC, especially among adolescents with high levels of earthquake exposure. Positive coping strategies may help adolescents manage stress after a severe earthquake. [Journal of Psychosocial Nursing and Mental Health Services, 60(11), 41-47.].

Charge Neutralization Strategy to Construct Salt-Tolerant and Cell-Permeable Nanoprobes: Application in Ratiometric Sensing and Imaging of Intracellular pH.

Intracellular pH homeostasis is essential for the survival and function of biological cells. Negatively charged molecular probes, such as pyranine (HPTS), tend to exhibit poor salt tolerance and unsatisfactory cell permeability, limiting their widespread use in intracellular assays. Herein, we explored a charge neutralization strategy using multicharged cationic nanocarriers for an efficient and stable assembly with the pH-sensitive HPTS. Through immobilization and neutralization with poly(allylamine hydrochloride)-stabilized red-emitting gold nanoclusters (PAH-AuNCs), the resulting nanoprobes (HPTS-PAH-AuNCs) offered improved salt tolerance, satisfactory cell permeability, and dual-emission properties. The fluorescence ratio exhibited a linear response over the pH range of 3.0-9.0. Moreover, the proposed HPTS-PAH-AuNCs were successfully applied to determine and visualize lysosomal pH variations in living cells, which indicated great potential for biosensing and bioimaging applications in living systems. Benefiting from the charge neutralization strategy, various types of probes can be expected to achieve broader analytical applications.

Recent Advances in Fluorescence Imaging of Traumatic Brain Injury in Animal Models.

Traumatic brain injury (TBI) is one of the top three specific neurological disorders, requiring reliable, rapid, and sensitive imaging of brain vessels, tissues, and cells for effective diagnosis and treatment. Although the use of medical imaging such as computed tomography (CT) and magnetic resonance imaging (MRI) for the TBI detection is well established, the exploration of novel TBI imaging techniques is of great interest. In this review, recent advances in fluorescence imaging for the diagnosis and evaluation of TBI are summarized and discussed in three sections: imaging of cerebral vessels, imaging of brain tissues and cells, and imaging of TBI-related biomarkers. Design strategies for probes and labels used in TBI fluorescence imaging are also described in detail to inspire broader applications. Moreover, the multimodal TBI imaging platforms combining MRI and fluorescence imaging are also briefly introduced. It is hoped that this review will promote more studies on TBI fluorescence imaging, and enable its use for clinical diagnosis as early as possible, helping TBI patients get better treatment and rehabilitation.