A simple Ag-MoS2 hybrid nanozyme-based sensor array for colorimetric identification of biothiols and cancer cells.

The intracellular levels of biothiols are associated with various diseases including cancer, and biothiols are regarded as tumor biomarker. Due to the similarity of the molecular structure of biothiols, the development of simple, rapid, efficient, and sensitive colorimetric sensor arrays holds great promise for clinical cancer diagnosis. Here, we developed a simple Ag-MoS2 hybrid nanozyme-based sensor array for colorimetric identification of biothiols and cancer cells. The novel Ag-MoS2 nanoprobe was synthesized in a simple and efficient way through the in situ self-reduction reaction between MoS2 and noble metal precursor. Benefiting from to the formation of heterogeneous metal structures, the peroxidase (POD)-like catalytic activity of the synthesized Ag-MoS2 hybrid nanocomposites is significantly enhanced compared to MoS2 alone. Moreover, the catalytic activity of Ag-MoS2 nanozyme was correlated with the pH of the reaction solution and the inhibitory effects of the three biothiols on the nanozyme-triggered chromogenic system differed in the specific pH environments. Therefore, each sensing unit of this electronic tongue generated differential colorimetric fingerprints of different biothiols. After principal component analysis (PCA), the developed novel colorimetric sensor array can accurately discriminate biothiols between different types, various concentrations, and different mixture proportions. Further, the sensor array was used for the colorimetric identification of real serum and cellular samples, demonstrating its great potential in tumor diagnostic applications.

Synthesis of Pt-MoS2 with enhanced photothermal and peroxidase-like properties and its antibacterial application.

Despite tremendous efforts, bacterial infection and contamination remain a major clinical challenge to modern humans. Nanozyme materials with stimuli-responsive properties are expected to be powerful tools for the next generation of antibacterial therapy. Here, MoS2 nanosheet was firstly prepared by liquid phase exfoliation method, and Pt-MoS2 hybrid biomaterial was then successfully synthesized by a simple self-reduction method. The Pt decoration significantly improves the photothermal effect of MoS2 nanosheet under 808 nm NIR laser irradiation. Besides, benefiting from the formation of heterogeneous structure, the Pt-MoS2 has significantly enhanced peroxidase mimetic catalytic activity, which can kill bacteria through catalysis of H2O2 to generate antimicrobial hydroxyl radicals. Moreover, the temperature rise brought about by NIR laser stimulation further amplifies the nanozyme activity of the composites. After treatment by the synergistic platform, both Staphylococcus aureus and Escherichia coli can be effectively inhibited, demonstrating its broad-spectrum antibacterial properties. In addition, the developed antibacterial Pt-MoS2 nanozyme have the excellent biocompatibility, which makes them well suited for infection elimination in biological systems. Overall, this work shows great potential for rationally combining the multiple functions of MoS2-based nanomaterials for synergistic antibacterial therapy. In the future, the Pt-MoS2 nanozyme may find wider applications in areas such as personal healthcare or surface disinfection treatment of medical devices.

'Microscopic engineering vehicles' for plants under stress combination.

The combination of stresses induced by climate change poses significant risks to crop production. We propose using 'microscopic engineering vehicles' in pesticides to mitigate biotic and abiotic stresses on plants. We discuss their customization and potential mode of action, which may help in addressing the crises caused by stress combination.

Efficient delivery of the herbicide quinclorac by nanosuspension for enhancing deposition, uptake and herbicidal activity.

BACKGROUND: The presence of barnyardgrass poses a threat to global food security by reducing rice yields. Currently, herbicides are primarily applied for weed management. However, the effectiveness of herbicide deposition and uptake on barnyardgrass is limited as a consequence of the high wax content on leaves, low water solubility and extreme lipophilicity of herbicides. Therefore, it is imperative to develop novel formulations for efficient delivery of herbicides to improve herbicidal activity and reduce dosage. RESULTS: We successfully prepared nanosuspension(s) (NS) of quinclorac through the wet media milling technique. This NS demonstrates excellent physical stability and maintains nanoscale during dose transfer. The deposition concentration and uptake concentration of NS on barnyardgrass were 3.84-4.47- and 2.11-2.58-fold greater than those traditional formulations, respectively. Moreover, the NS exhibited enhanced herbicidal activity against barnyardgrass at half the dosage required by conventional formulations without compromising rice safety. CONCLUSIONS: These findings suggest that NS can effectively facilitate the delivery of hydrophobic and poorly water-soluble herbicide active ingredients, thereby enhancing their deposition, uptake and bioactivity. This study expands the potential application of NS in pesticide delivery, which can provide valuable support for optimizing pesticide utilization, improving economic efficiency and mitigating environmental risks. © 2024 Society of Chemical Industry.

Improving the efficiency and environmental safety of emamectin benzoate through a pH-responsive metal-organic framework microencapsulation strategy.

Herein, we developed a technique for loading nanopesticides onto Metal-Organic Frameworks (MOFs) to control Spodoptera litura. The average short-axis length of the synthesized carrier emamectin benzoate@PCN-222 @hyaluronic acid (EB@PCN-222 @HA) was ∼40 nm, with an average long-axis length of ∼80 nm. This enabled the manipulation of its size, contact angle, and surface tension on the surface of leaves. Pesticide-loading capacity, determined via thermogravimetric analysis, was measured at ∼16 %. To ensure accurate pesticide release in the alkaline intestine of Spodoptera litura, EB@PCN-222 @HA was engineered to decompose under alkaline conditions. In addition, the carrier delayed the degradation rate of EB, enhancing EB's stability. Loading Nile red onto PCN-222 @HA revealed potential entry into the insect body through feeding, which was supported by bioassay experiments. Results demonstrated the sustained-release performance of EB@PCN-222 @HA, extending its effective duration. The impact of different carrier concentrations on root length, stem length, fresh weight, and germination rate of pakchoi and tomato were assessed. Promisingly, the carrier exhibited a growth-promoting effect on the fresh weight of both the crops. Furthermore, cytotoxicity experiments confirmed its safety for humans. In cytotoxicity assays, PCN-222 @HA showed minimal toxicity at concentrations up to 100 mg/L, with cell survival rates above 80 %. Notably, the EB@PCN-222 @HA complex demonstrated reduced cytotoxicity compared to EB alone, supporting its safety for human applications. This study presents a safe and effective approach for pest control using controlled-release pesticides with extended effective durations.

Making the Complicated Simple: A Minimizing Carrier Strategy on Innovative Nanopesticides.

The flourishing progress in nanotechnology offers boundless opportunities for agriculture, particularly in the realm of nanopesticides research and development. However, concerns have been raised regarding the human and environmental safety issues stemming from the unrestrained use of non-therapeutic nanomaterials in nanopesticides. It is also important to consider whether the current development strategy of nanopesticides based on nanocarriers can strike a balance between investment and return, and if the complex material composition genuinely improves the efficiency, safety, and circularity of nanopesticides. Herein, we introduced the concept of nanopesticides with minimizing carriers (NMC) prepared through prodrug design and molecular self-assembly emerging as practical tools to address the current limitations, and compared it with nanopesticides employing non-therapeutic nanomaterials as carriers (NNC). We further summarized the current development strategy of NMC and examined potential challenges in its preparation, performance, and production. Overall, we asserted that the development of NMC systems can serve as the innovative driving force catalyzing a green and efficient revolution in nanopesticides, offering a way out of the current predicament.

Nano-Micro Core-Shell Fibers for Efficient Pest Trapping.

Insect sex pheromones as an alternative to chemical pesticides hold promising prospects in pest control. However, their burst release and duration need to be optimized. Herein, pheromone-loaded core-shell fibers composed of degradable polycaprolactone and polyhydroxybutyrate were prepared by coaxial electrospinning. The results showed that this core-shell fiber had good hydrophobic performance and thermal stability, and the light transmittance in the ultraviolet band was only below 40%, which provided protection to pheromones. The core-shell structure alleviated the burst release of pheromone in the fiber and extended the release time to about 133 days. In the field, the pheromone-loaded core-shell fibers showed the same continuous and efficient trapping of Spodoptera litura as the commercial carriers. More importantly, the electrospun fibers combined with biomaterials had a degradability unmatched by commercial carriers. The structure design strategy provides ideas for the innovative design of pheromone carriers and is a potential tool for the management of agricultural pests.

Multifunctional Borax Cross-Linked Hydroxypropyl Guar Gum Hydrogels with Crop Nutritional Function as Carriers for Dual-Responsive Acaricide Release.

Hydrogels with porous networks have received considerable attention in smart pesticide delivery due to their inherent versatility. In this study, acaricide cyetpyrafen (CPF)-loaded borax (BO) cross-linked hydroxypropyl guar gum (HPG) (CPF@BO-co-HPG, CBG) hydrogels were prepared by cross-linking and pesticide loading simultaneously. The flowable CBG hydrogels with 3D porous network structures had better wetting and spreading ability on Citrus reticulata Blanco leaves and a hydrophobic interface. The nonflowable CBG hydrogels had pH- and temperature-responsive release properties. Meanwhile, the acaricidal efficacy of CBG against Panonychus citri (McGregor) at both 24 and 48 h was significantly higher than those of CPF-loaded BO-free HPG hydrogels. Furthermore, CBG had a nutritional function for cotton growth and environmental safety for zebrafish. This research developed a BO cross-linked HPG hydrogel as a smart pesticide delivery vehicle and crop nutrient replenishment, which can be widely applied in sustainable agriculture.

Biodegradable electrospun fibers as sustained-release carriers of insect pheromones for field trapping of Spodoptera litura (Lepidoptera: Noctuidae).

BACKGROUND: Insect pheromones are highly effective and environmentally friendly, and are widely used in the monitoring and trapping of pests. However, many researchers have found that various factors such as ultraviolet light and temperature in the field environment can accelerate the volatilization of pheromones, thus affecting the actual control effect. In recent years, electrospinning technology has demonstrated remarkable potential in the preparation of sustained carriers. Moreover, the utilization of biodegradable materials in electrospinning presents a promising avenue for the advancement of eco-friendly carriers. RESULTS: In this study, homogeneous and defect-free pheromone carriers were obtained by electrospinning using fully biodegradable polyhydroxybutyrate materials and pheromones of Spodoptera litura. The electrospun fibers with porous structure could continuously release pheromone (the longest can be ≤80 days). They also had low light transmission, hydrophobic protection. More importantly, the pheromone-loaded electrospun fiber carriers showed stable release and good trapping effect in the field. They could trap pests for at least 7 weeks in the field environment without other light stabilizers added. CONCLUSION: Sustained-release carriers constructed by electrospinning and green materials could improve the efficacy of pheromones and ensure environmental friendliness, and provided a tool for the management of S. litura and other pests and sustainable development of agricultural. © 2023 Society of Chemical Industry.

Preparation of Thifluzamide Polylactic Acid Glycolic Acid Copolymer Microspheres and Its Effect on the Growth of Cucumber Seedlings.

The polylactic acid-glycolic acid copolymer (PLGA) has been proven to be applicable in medicine, but there is limited research on its application and safety in the agricultural field. In this paper, thifluzamide PLGA microspheres were prepared via phacoemulsification and solvent volatilization, using the PLGA copolymer as the carrier and thifluzamide as the active component. It was found that the microspheres had good slow-release performance and fungicidal activity against Rhizoctonia solani. A comparative study was conducted to show the effect of thifluzamide PLGA microspheres on cucumber seedlings. Physiological and biochemical indexes of cucumber seedlings, including dry weight, root length, chlorophyll, protein, flavonoids, and total phenol content, indicated that the negative effect of thifluzamide on plant growth could be mitigated when it was wrapped in PLGA microspheres. This work explores the feasibility of PLGA as carriers in fungicide applications.

Metabolic rearrangements and intratumoral heterogeneity for immune response in hepatocellular carcinoma.

Liver cancer is one of the most common malignant tumors globally. Not only is it difficult to diagnose, but treatments are scarce and the prognosis is generally poor. Hepatocellular carcinoma (HCC) is the most common type of liver cancer. Aggressive cancer cells, such as those found in HCC, undergo extensive metabolic rewiring as tumorigenesis, the unique feature, ultimately causes adaptation to the neoplastic microenvironment. Intratumoral heterogeneity (ITH) is defined as the presence of distinct genetic features and different phenotypes in the same tumoral region. ITH, a property unique to malignant cancers, results in differences in many different features of tumors, including, but not limited to, tumor growth and resistance to chemotherapy, which in turn is partly responsible for metabolic reprogramming. Moreover, the different metabolic phenotypes might also activate the immune response to varying degrees and help tumor cells escape detection by the immune system. In this review, we summarize the reprogramming of glucose metabolism and tumoral heterogeneity and their associations that occur in HCC, to obtain a better understanding of the mechanisms of HCC oncogenesis.

The connection between innervation and metabolic rearrangements in pancreatic cancer through serine.

Pancreatic cancer is a kind of aggressive tumor famous for its lethality and intractability, and pancreatic ductal adenocarcinoma is the most common type. Patients with pancreatic cancer often suffer a rapid loss of weight and abdominal neuropathic pain in their early stages and then go through cachexia in the advanced stage. These features of patients are considered to be related to metabolic reprogramming of pancreatic cancer and abundant nerve innervation responsible for the pain. With increasing literature certifying the relationship between nerves and pancreatic ductal adenocarcinoma (PDAC), more evidence point out that innervation's role is not limited to neuropathic pain but explore its anti/pro-tumor functions in PDAC, especially the neural-metabolic crosstalks. This review aims to unite pancreatic cancer's innervation and metabolic rearrangements with terminated published articles. Hopefully, this article could explore the pathogenesis of PDAC and further promote promising detecting or therapeutic measurements for PDAC according to the lavish innervation in PDAC.

Bioinformatic identification of differentially expressed genes associated with hepatocellular carcinoma prognosis.

Hepatocellular carcinoma (HCC) is still a significant global health problem. The development of bioinformatics may provide the opportunities to identify novel therapeutic targets. This study bioinformatically identified the differentially expressed genes (DEGs) in HCC and associated them with HCC prognosis using data from published databases. The DEGs downloaded from the Gene Expression Omnibus (GEO) website were visualized using the Venn diagram software, and then subjected to the GO and KEGG analyses, while the protein-protein interaction network was analyzed using Cytoscape software with the Search Tool for the search tool for the retrieval of interacting genes and the molecular complex detection plug-in. Kaplan-Meier curves and the log rank test were used to associate the core PPI network genes with the prognosis. There were 57 upregulated and 143 downregulated genes in HCC samples. The GO and pathway analyses revealed that these DEGs are involved in the biological processes (BPs), molecular functions (MFs), and cell components (CCs). The PPI network covered 50 upregulated and 108 downregulated genes, and the core modules of this PPI network contained 34 upregulated genes. A total of 28 of these upregulated genes were associated with a poor HCC prognosis, 27 of which were highly expressed in HCC tissues. This study identified 28 DEGs to be associated with a poor HCC prognosis. Future studies will investigate their possible applications as prognostic biomarkers and potential therapeutic targets for HCC.

Metabolic pathways reveal the effect of fungicide loaded metal-organic frameworks on the growth of wheat seedlings.

Metal-organic frameworks (MOF) are an emerging class of hybrid inorganic-organic porous materials used in various fields, especially in molecule delivery system. As iron is an essential micronutrient for plant growth, iron-based MOF (Fe-MOF) is developed for agricultural application as fungicide carriers. However, fungicides may have various effect on the plant growth, which may be different from Fe-MOF. When they are combined with the carriers, the effects on target plants will change. In this work, tebuconazole-loaded Fe-MOF was prepared and used to treat wheat seedlings. The physiological, biochemical and metabolic levels of wheat roots and shoots were shown by a comparative study. Related metabolic pathways were analyzed by non-targeted metabolomic method. Many metabolites in wheat roots and shoots showed an upward trend after Fe-MOF treatment, but tebuconazole had a negative impact on these indicators. Related metabolic pathways in Fe-MOF and tebuconazole treatment were different, and the related pathway of tebuconazole-loaded Fe-MOF was closer to that of Fe-MOF. The metabolic pathways study revealed that the negative impact from tebuconazole was mitigated when wheat seedlings were treated with tebuconazole-loaded Fe-MOF. This research firstly explores the mechanism of MOF as carriers to help plant reduce the negative effects from fungicide by regulating metabolic pathways.

Effects of Methoxyfenozide-Loaded Fluorescent Mesoporous Silica Nanoparticles on Plutella xylostella (L.) (Lepidoptera: Plutellidae) Mortality and Detoxification Enzyme Levels Activities.

The diamond back moth, Plutella xylostella, causes severe damage at all crop stages, beside its rising resistance to all insecticides. The objective of this study was to look for a new control strategy such as application of insecticide-loaded carbon dot-embedded fluorescent mesoporous silica nanoparticles (FL-SiO2 NPs). Two different-sized methoxyfenozide-loaded nanoparticles (Me@FL-SiO2 NPs-70 nm, Me@FL-SiO2 NPs-150 nm) were prepared, with loading content 15% and 16%. Methoxyfenozide was released constantly from Me@FL-SiO2 NPs only at specific optimum pH 7.5. The release of methoxyfenozide from Me@FL-SiO2 NPs was not observed other than this optimum pH, and therefore, we checked and controlled a single release condition to look out for the different particle sizes of insecticide-loaded NPs. This pH-responsive release pattern can find potential application in sustainable plant protection. Moreover, the lethal concentration of the LC50 value was 24 mg/L for methoxyfenozide (TC), 14 mg/L for Me@FL-SiO2 NPs-70 nm, and 15 mg/L for Me@FL-SiO2 NPs-150 nm after 72 h exposure, respectively. After calculating the LC50, the results predicted that Me@FL-SiO2 NPs-70 nm and Me@FL-SiO2 NPs-150 nm exhibited better insecticidal activity against P. xylostella than methoxyfenozide under the same concentrations of active ingredient applied. Moreover, the activities of detoxification enzymes of P. xylostella were suppressed by treatment with insecticide-loaded NPs, which showed that NPs could also be involved in reduction of enzymes. Furthermore, the entering of FL-SiO2 NPs into the midgut of P. xylostella was confirmed by confocal laser scanning microscope (CLSM). For comparison, P. xylostella under treatment with water as control was also observed under CLSM. The control exhibited no fluorescent signal, while the larvae treated with FL-SiO2 NPs showed strong fluorescence under a laser excitation wavelength of 448 nm. The reduced enzyme activities as well as higher cuticular penetration in insects indicate that the nano-based delivery system of insecticide could be potentially applied in insecticide resistance management.

Complement regulation in ovine lymph nodes during early pregnancy.

A fetus changes immune responses in the uterus and the maternal immune system, and lymph nodes are associated with regulating maternal adaptive immunity. Complement activation is associated with abnormal pregnancy in mice and humans. The aim of the present study was to explore the expression levels of complement components in maternal lymph nodes during early pregnancy in sheep. Maternal inguinal lymph nodes were sampled on day 16 of the estrous cycle, and days 13, 16 and 25 of gestation in ewes. Reverse transcription-quantitative PCR, western blotting and immunohistochemical analyses were used to detect the expression levels of complement components C1q, C1r, C1s, C2, C3, C4a, C5b and C9 in the lymph nodes. The results revealed that the protein and mRNA levels of C1q, C1s and C5b were enhanced during early pregnancy, and that C1r and C4a were upregulated at day 25 of pregnancy. The mRNA and protein levels of C2 and C9 peaked at day 16 of pregnancy, but C3 was decreased at day 25 of pregnancy. C3 protein was located in the subcapsular sinuses and lymph sinuses of the maternal lymph node. In summary, the present study detected changes in the expression levels of complement components in maternal lymph nodes, which may be associated with maternal immune regulation during early pregnancy in sheep.

Fungicide-loaded mesoporous silica nanoparticles promote rice seedling growth by regulating amino acid metabolic pathways.

Mesoporous silica nanoparticles (MSN) are widely researched as carriers for pesticides (including fungicides, insecticides and herbicides) to improve their effective utilization rate in the target plant. However, pesticides enter the target crops and may bring some impacts on the growth and physiological function of plants. When they are loaded to nanoparticles, different effects on the metabolic properties of target plants will be produced. In this study, thifluzamide-loaded MSN was prepared with average diameter of 80-120 nm. Rice seedlings were exposed for 7 days to different treatments of MSN, thifluzamide, and thifluzamide-loaded MSN. After treatment, non-targeted metabolomic method was employed to explore the metabolic pathways. It was found that the negative effect of thifluzamide to rice seedling was alleviated by thifluzamide-loaded MSN, since it increased amino acid metabolic pathways, which improved purine and pyrimidine metabolism and induced the production of total protein. Thifluzamide-loaded MSN can also relieve the damage of thifluzamide to rice seedlings by altering the chlorophyll, phenols and flavonoids content. In conclusion, it was proposed that the mechanism of fungicide-loaded MSN prevent plant from negative effects of fungicides by regulating the amino acid metabolic pathways.

TC2N: A Novel Vital Oncogene or Tumor Suppressor Gene In Cancers.

Several C2 domain-containing proteins play key roles in tumorigenesis, signal transduction, and mediating protein-protein interactions. Tandem C2 domains nuclear protein (TC2N) is a tandem C2 domain-containing protein that is differentially expressed in several types of cancers and is closely associated with tumorigenesis and tumor progression. Notably, TC2N has been identified as an oncogene in lung and gastric cancer but as a tumor suppressor gene in breast cancer. Recently, a large number of tumor-associated antigens (TAAs), such as heat shock proteins, alpha-fetoprotein, and carcinoembryonic antigen, have been identified in a variety of malignant tumors. Differences in the expression levels of TAAs between cancer cells and normal cells have led to these antigens being investigated as diagnostic and prognostic biomarkers and as novel targets in cancer treatment. In this review, we summarize the clinical characteristics of TC2N-positive cancers and potential mechanisms of action of TC2N in the occurrence and development of specific cancers. This article provides an exploration of TC2N as a potential target for the diagnosis and treatment of different types of cancers.

Enhanced Fungicidal Efficacy by Co-Delivery of Azoxystrobin and Diniconazole with Cauliflower-Like Metal-Organic Frameworks NH2-Al-MIL-101.

Long-term use of a single fungicide increases the resistance risk and causes adverse effects on natural ecosystems. Controlled release formulations of dual fungicides with different modes of action can afford a new dimension for addressing the current issues. Based on adjustable aperture and superhigh surface area, metal-organic frameworks (MOFs) are ideal candidates as pesticide release carriers. This study used Al3+ as the metal node and 2-aminoterephthalic acid as the organic chain to prepare aluminum-based metal-organic framework material (NH2-Al-MIL-101) with "cauliflower-like" structure and high surface area of 2359.0 m2/g. Fungicides of azoxystrobin (AZOX) and diniconazole (Dini) were simultaneously encapsulated into NH2-Al-MIL-101 with the loading content of 6.71% and 29.72%, respectively. Dual fungicide delivery system of AZOX@Dini@NH2-Al-MIL-101 demonstrated sustained and pH responsive release profiles. When the maximum cumulative release rate of AZOX and Dini both reached about 90%, the release time was 46 and 136 h, respectively. Furthermore, EC50 values as well as the percentage of inhibition revealed that AZOX@Dini@NH2-Al-MIL-101 had enhanced germicidal efficacy against rice sheath blight (Rhizoctonia solani), evidenced by the synergistic ratio of 1.83. The present study demonstrates a potential application prospect in sustainable plant protection through co-delivery fungicides with MOFs as a platform.