ABSTRACT
We report a study on inhibition of human oral squamous cell carcinoma in vitro and in vivo, using novel photosensitizer (PS) aloe emodin (AE) mediated photodynamic therapy (PDT). Distinct morphology changes of oral mucosa carcinoma KB cells were observed under an optical microscope and cell migrations were inhibited owing to AE-PDT. The cell proliferation was blocked in G1 phase and the apoptosis increase were both caused by massive reactive oxygen species (ROS) generated from photoactivated AE. The upregulation of Caspase-3 and Bax protein levels and downregulation of Bcl-2 protein levels were observed after AE-PDT. The survival time of tumor mouse was prolonged without side effects ascribed to AE-PDT and its inhibitory effect on mice transplantation tumors was significant. It is indicated that AE mediated PDT is an innovative way to oral cancer treatment with the dominances of effectivity, minimal invasion, tissue integrity retention and none side effects on main organs.
Subject(s)
Anthraquinones/administration & dosage , Carcinoma, Squamous Cell/drug therapy , Mouth Neoplasms/drug therapy , Photochemotherapy/methods , Animals , Anthraquinones/adverse effects , Anthraquinones/pharmacology , Apoptosis/drug effects , Carcinoma, Squamous Cell/pathology , Cell Movement/drug effects , Cell Proliferation/drug effects , Gene Expression Regulation, Neoplastic , Humans , Male , Mice , Mice, Inbred BALB C , Mice, Nude , Mouth Mucosa/pathology , Mouth Neoplasms/pathology , Neoplasm Transplantation , Photochemotherapy/adverse effects , Photosensitizing Agents/administration & dosage , Photosensitizing Agents/adverse effects , Photosensitizing Agents/pharmacology , Reactive Oxygen Species/metabolism , Survival RateABSTRACT
Two lanthanide complexes, Ln(HPDA)(3)·4EtOH (Ln = Tb, Dy) (H(2)PDA = pyridine-2,6-dicarboxylic acid, EtOH = ethanol), have been successfully synthesized using hydrothermal or solvothermal methods, and their crystal structures were analyzed by single crystal XRD. Both crystals have orthorhombic symmetry with space group Pbcn, exhibiting three-dimensional (3D) supramolecular architecture through hydrogen bonding interactions. The metal center was coordinated to nine atoms by three pyridine-2,6-dicarboxylic acid ligands. The nine-coordinated lanthanide metal complexes were assembled onto a nanocrystalline TiO(2) film to form co-sensitized photoelectrodes with N719 for dye-sensitized solar cells, and their photoelectrochemical performance was studied. In the tandem structure of composite electrodes, the energy levels of lanthanide metal complexes are reorganized in their single-crystal form, as verified by ab initio calculations. The co-sensitized systems are far superior for electron-injection and hole-recovery compared with single N719-sensitized systems. Luminescence properties were measured and electrochemical analysis was also performed on these complexes.
