Deficiency of ValRS-m Causes Male Infertility in Drosophila melanogaster.

Drosophila spermatogenesis involves the renewal of germline stem cells, meiosis of spermatocytes, and morphological transformation of spermatids into mature sperm. We previously demonstrated that Ocnus (ocn) plays an essential role in spermatogenesis. The ValRS-m (Valyl-tRNA synthetase, mitochondrial) gene was down-regulated in ocn RNAi testes. Here, we found that ValRS-m-knockdown induced complete sterility in male flies. The depletion of ValRS-m blocked mitochondrial behavior and ATP synthesis, thus inhibiting the transition from spermatogonia to spermatocytes, and eventually, inducing the accumulation of spermatogonia during spermatogenesis. To understand the intrinsic reason for this, we further conducted transcriptome-sequencing analysis for control and ValRS-m-knockdown testes. The differentially expressed genes (DEGs) between these two groups were selected with a fold change of ≥2 or ≤1/2. Compared with the control group, 4725 genes were down-regulated (dDEGs) and 2985 genes were up-regulated (uDEGs) in the ValRS-m RNAi group. The dDEGs were mainly concentrated in the glycolytic pathway and pyruvate metabolic pathway, and the uDEGs were primarily related to ribosomal biogenesis. A total of 28 DEGs associated with mitochondria and 6 meiosis-related genes were verified to be suppressed when ValRS-m was deficient. Overall, these results suggest that ValRS-m plays a wide and vital role in mitochondrial behavior and spermatogonia differentiation in Drosophila.

Modelling urban flooding integrated with flow and sediment transport in drainage networks.

Drainage networks play an essential role in mitigating urban flooding, which, nevertheless, are prone to suffer sediment deposits. To date, however, the effects of sediments in drainage networks on urban flooding remain poorly understood. Here an integrated model is proposed for urban flooding. It is composed of a hydrological module for surface runoff integrated with a one-dimensional hydro-sediment-morphodynamic module for coupled open-channel or pressurized flow and sediment transport in drainage networks. The governing equations are solved synchronously using a well-balanced finite volume method. The model is tested against two laboratory cases involving mixed flow and sediment transport in pipes, and the results agree well with observed data. A new residential area with virtually pervious surface and an established urban area with essentially impervious surfaces are studied using the present model to unravel how sediments in drainage networks affect urban flooding under different extreme rainfall and sediment scenarios. The results reveal that sediments alter the discharge hydrographs in the drainage networks to distinct extents under different storm return periods. As far as the present computational cases are concerned, when a third of the pipe diameter is occupied by sediment deposits, the peak pipeline flow discharge decreases by up to 25 %. Accordingly, the surface inundation depth increases by up to 18 %, and the inundation area expands by up to 12 %, characterizing a considerably higher flooding risk. The present findings provide insight into the influences of sediment transport in drainage networks on urban flooding.

Coupled modelling of flow and non-capacity sediment transport in sewer flushing channel.

Flushing is cost-effective for mitigating sediments and constraining environmental problems in sewer systems. Previous mathematical models are almost exclusively built upon simplified governing equations evoking the assumptions of slow bed evolution and sediment transport capacity, of which the applicability remains open to question. Here a 1D coupled non-capacity model is presented for non-uniform sediment transport in sewer flushing channels, as adapted from recently established shallow water hydro-sediment-morphodynamic models for fluvial processes. The present model is tested for an experimental flushing case in Paris's Des Coteaux catchment system. The computational results agree with observed data more closely than those of a previous decoupled capacity model. While the differences between decoupled and capacity models and the present coupled non-capacity model are minor for flushing processes of short-durations, they are more pronounced for sustained long-duration flushing processes. Physically, the adaptation of suspended sediments to capacity regime cannot be fulfilled quickly, though bedload sediments can adapt to capacity regime instantly. Also, the bed deformation rate is comparable to its counterpart of the flow depth. Therefore, coupled non-capacity modelling is suggested for general applications to sewer flushing channels, of which the computing cost is essentially equivalent to simplified models built upon decoupling and capacity assumptions.