Ordered porous nitrogen-doped carbon with atomically dispersed FeN4 for efficient oxygen reduction reaction in microbial fuel cell.

It is necessary to develop cost-effective platinum group materials (PGM)-free cathode catalysts for the oxygen reduction reaction (ORR) to replace the state-of-the-art noble metal for the commercialization of microbial fuel cells (MFCs). Fe-N-C catalysts are one of the promising candidates for commercial Pt/C. Herein, a new cost-effective and highly stable Fe-N-C catalyst was designed and successfully prepared via a facile process. The catalyst was prepared through one single thermal conversion from Fe-doped ZIF-8 (zeolitic imidazolate framework), a metal-organic framework (MOF) containing well-defined FeN4 coordination. The mesopores in the unique structure of MOF promoted efficient mass transfer, while those arising from the pyrolysis of the MOF produced more active sites. Unlike previous preparation methods, post-treatments were avoided here. As a result, the Fe-N-C cathode had a high ORR activity with a half-wave potential (E1/2) of 0.85 V in a 0.1 M KOH aqueous solution. The classic MFC with this cathodic catalyst provided a maximum power density of up to 1508 mW m-2.

Development of a Novel Urine Alzheimer-Associated Neuronal Thread Protein ELISA Kit and Its Potential Use in the Diagnosis of Alzheimer's Disease.

BACKGROUND: Alzheimer's disease (AD) is an age-related chronic degenerative disease that damages the nervous system. A noninvasive and simple method for early detection of AD is extremely important for the diagnosis and prognosis of AD. Thus, we aimed to develop an enzyme-linked immunosorbent assay (ELISA) kit to detect urine Alzheimer-associated neuronal thread protein (AD7C-NTP), and to evaluate its clinical value for the diagnosis of AD. METHODS: Immunogenic AD7C-NTP peptide fragments were synthesized by the solid-phase method and used for immunizing mice or rabbits to generate anti-AD7C-NTP antibodies. The urine AD7C-NTP ELISA kit was then established; the generated mouse anti-AD7C-NTP antibody was used as a capture antibody, the biotin-labeled rabbit anti-AD7C-NTP antibody was used as a detection antibody, and avidin labeled by horseradish peroxidase was used as a substrate. The first morning urine specimens of 121 AD patients and 118 age-matched controls were collected, and the urine AD7C-NTP levels were detected by the above ELISA kit. RESULTS: Mouse and rabbit anti-AD7C-NTP antibody ELISA titer was found to be 1:8,000 and 1:32,000, respectively. A single band with a relative molecular mass of 41 kDa was found in human brain specimens by Western blot assay, which was identified as AD7C-NTP antibody. The urine AD7C-NTP concentration of the AD patients was higher than that of the age-matched controls, the sensitivity was 89.3% and the specificity was 84.7%. CONCLUSIONS: Our study demonstrated that our newly developed urine AD7C-NTP ELISA kit has suggested potential for diagnosing AD in a Chinese population, suggesting it may be a useful diagnostic kit for detecting early AD.