ABSTRACT
The in vitro responses of channel catfish peripheral blood neutrophils to Edwardsiella ictaluri were examined through the use of phagocytic, bactericidal, and chemiluminescent assays. Evidence from both light and electron microscopy indicate that catfish neutrophils appeared to phagocytose E. ictaluri. Although extracellular killing of E. ictaluri was observed, bactericidal assays did not demonstrate intracellular killing of E. ictaluri by neutrophils. Catfish neutrophils mount a chemiluminescent response to E. ictaluri which is enhanced by the presence of specific antibody. Incubation of catfish neutrophils with LPS from E. ictaluri appeared to suppress the chemiluminescent response to opsonized zymosan.
Subject(s)
Enterobacteriaceae/immunology , Ictaluridae/immunology , Neutrophils/physiology , Animals , Antibodies, Bacterial/immunology , Bacteriolysis , Cells, Cultured , Lipopolysaccharides/pharmacology , Luminescent Measurements , Neutrophils/drug effects , Phagocytosis , Zymosan/pharmacologyABSTRACT
Two monoclonal antibodies produced against Streptococcus agalactiae were studied for their specificity and sensitivity. Both reacted strongly in the enzyme-linked immunosorbent assay and indirect fluorescent antibody test with S agalactiae, but did not react with other gram-positive organisms frequently found in test herds in Mississippi. A procedure for using monoclonal antibodies in the detection of S agalactiae-infected milk samples is proposed.
Subject(s)
Antibodies, Monoclonal/immunology , Antigens, Bacterial/immunology , Streptococcus agalactiae/immunology , Animals , Cattle , Enzyme-Linked Immunosorbent Assay , Female , Fluorescent Antibody Technique , Mastitis, Bovine/diagnosis , Mastitis, Bovine/immunology , Mastitis, Bovine/microbiologyABSTRACT
Three monoclonal antibodies produced against Leptospira interrogans serovar pomona have been studied for their diagnostic usefulness. All three monoclonals reacted strongly in the enzyme-linked immunosorbent assay and indirect fluorescent antibody test with serovar pomona and did not react with serovars grippotyphosa, canicola, icterohaemorrhagiae and hardjo.