ABSTRACT
Overwhelming evidence supports the theory that inflammatory bowel disease (IBD) is caused by a complex interplay between genetic predispositions of multiple genes, combined with an abnormal interaction with environmental factors. It is becoming apparent that epigenetic factors can have a significant contribution in the pathogenesis of disease. Changes in the methylation state of IBD-associated genes could significantly alter levels of gene expression, potentially contributing to disease onset and progression. We have explored the role of DNA methylation in IBD pathogenesis. DNA methylation profiles (1505 CpG sites of 807 genes) of matched diseased (n = 26) and non-diseased (n = 26) intestinal tissues from 26 patients with IBD [Crohn's disease (CD) n = 9, ulcerative colitis (UC) n = 17] were profiled using the GoldenGate™ methylation assay. After an initial identification of a panel of 50 differentially methylated CpG sites from a training set (14 non-diseased and 14 diseased tissues) and subsequent validation with a testing set (12 non-diseased and 12 diseased tissues), we identified seven CpG sites that are differentially methylated in intestinal tissues of IBD patients. We have also identified changes in DNA methylation associated with the two major IBD subtypes, CD and UC. This study reports IBD-associated changes in DNA methylation in intestinal tissue, which may be disease subtype-specific.
Subject(s)
DNA Methylation , Inflammatory Bowel Diseases/genetics , Intestinal Mucosa/metabolism , Cluster Analysis , Colitis, Ulcerative/genetics , CpG Islands , Crohn Disease/genetics , Epigenesis, Genetic , Female , Gene Expression Profiling , Humans , MaleABSTRACT
Since the introduction in the Federal Republic of Germany of free routine screening for colorectal carcinoma (digital examination of the rectum in 1971; test for occult blood in the faeces in 1977), the number of Dukes' stage A cases diagnosed at one hospital has increased by 30%. The average interval between onset of symptoms and diagnosis has shortened from 5 to 3.8 months, and the proportion of inoperable carcinomas has decreased by 10%.
Subject(s)
Colonic Neoplasms/epidemiology , Mass Screening , Rectal Neoplasms/epidemiology , Germany, West , Humans , Neoplasm Staging , Retrospective StudiesABSTRACT
Since screening for colorectal carcinoma was started in Germany (rectal digital palpation 1971, test for occult blood in stool 1977) the rate of early stage Dukes A in more than 3600 cases of colorectal carcinoma has increased significantly by 30%. The history of the disease has shortened and the number of inoperable patients has diminished by 10%.
Subject(s)
Colonic Neoplasms/prevention & control , Mass Screening , Rectal Neoplasms/prevention & control , Colonic Neoplasms/pathology , Colonic Neoplasms/surgery , Humans , Neoplasm Staging , Prognosis , Rectal Neoplasms/pathology , Rectal Neoplasms/surgerySubject(s)
Gastrectomy , Stomach Neoplasms/etiology , Stomach Ulcer/surgery , Humans , Postoperative Complications/etiology , RiskABSTRACT
Indication, standard operative procedure and technique of ambulant diagnostic mammary surgery is shown in detail from 15 years of experience. In respect of reducing costs intraoperative quick-frozen sections and in case of malignancy radical mastectomy immediately after biopsy during continuous general anesthesia is important. By retrospective analysis we could prove that operative results in treatment of benign breast tumors by out-patient surgery or otherwise for in-patients do not differ. According to an inquiry patients preferred by 60% out-patient procedure.
Subject(s)
Biopsy/methods , Breast Neoplasms/diagnosis , Aftercare , Ambulatory Surgical Procedures , Female , Humans , Patient Acceptance of Health CareABSTRACT
After presentation of the causes, pathophysiology, diagnostic procedures and therapy, the results of treatment of 455 patients with mechanical ileus operated in the period 1964--1978 are presented.
Subject(s)
Intestinal Obstruction/surgery , Adult , Female , Humans , Intestinal Obstruction/diagnosis , Intestinal Obstruction/etiology , Intestine, Large , Intestine, Small , Male , Middle Aged , Postoperative ComplicationsABSTRACT
Autoradiographic and liquid scintillation counting studies were made during the early graft-versus-host-reaction in mice. At the height of GVHR on the 10th day after transplantation, hepatosplenomegaly correlated with the radioactive disintegrations (DPM). This coincided with a high proliferation rate of the blasts that appeared in the spleen, and of the portal infiltrations and Kupffer cells in the liver. Convenient control groups were used to show that these changes are a specific reaction in GVHR.
