Can Italian wines outperform European benchmarks in environmental impact? An examination through the product environmental footprint method.

Nowadays, there is a pressing demand for precise tools to quantify sustainability and assess the contributions of products and processes to sustainable development. This requirement extends to the wine industry as well. In 2013, the European Commission introduced the Product Environmental Footprint (PEF), providing a standardized methodology grounded in life cycle thinking for evaluating the environmental impacts of products across various industries. Despite its potential and the availability of specific guidelines for wine, the application of PEF remains unexplored in the sector. This study contributes to the knowledge by applying PEF to assess environmental impacts of Italian still and sparkling wines production, identifying variations from European benchmarks. Additionally, it aims to pinpoint pivotal hotspots and provide guidance for effective mitigation strategies. Average data from 27 wines certified as sustainable under Italian VIVA program, were collected and used to perform a life cycle assessment in accordance with PEF protocol. Results revealed that Italian still wines exhibited a greater environmental impact than the European reference value, while sparkling wines displayed a slightly more favourable environmental performance compared to the European average. Notably, specific impact categories remained consistent across different wines and countries, with climate change, resource utilization, land use, and particulate matter harmful for human health accounting for nearly 80 % of the overall environmental footprint. Hotspot analysis identified the plantation/destruction of vines, energy usage in the winery, and packaging as significant factors influencing the environmental footprint of Italian wines. Addressing these elements could enhance the environmental competitiveness of the Italian wine sector relative to its European counterparts. However, to validate these findings, further studies are necessary, both within Italy and in other European wine-producing regions. Such research initiatives will improve and strengthen PEF methodology, bolstering its adoption as the primary tool for environmental impact assessment of wine at the community level.

Glyphosate, glufosinate ammonium, and AMPA occurrences and sources in groundwater of hilly vineyards.

Glyphosate [N-(phosphonomethyl) glycine] and glufosinate ammonium [ammonium dl-homoalanin-4-(methyl) phosphinate] are broad-spectrum, nonselective, post-emergence herbicides extensively used in various applications for weed control in both agricultural and non-crop areas. Aminomethylphosphonic acid (AMPA) is the major degradation product of glyphosate found in plants, water, and soil. Due to glyphosate's presumed low mobility, its monitoring in European water was limited. Recently both glyphosate and AMPA have been detected in several groundwater samples in Europe, U.S, Canada, Argentina, and China. Understanding the sources of these substances in water, especially in groundwater used for drinking, becomes a priority. In the present work the occurrences and the main drives of glyphosate, AMPA, and glufosinate ammonium in the groundwater of hilly vineyards located in the North-West of Italy were evaluated. Groundwater monitoring results showed frequent detection and concentrations above EQSGW for glyphosate and AMPA, while glufosinate ammonium was never detected. More frequent occurrences and higher concentrations were detected in the samples collected from wells located in the farmyards, most of them being used for irrigation and/or preparation of PPPs mixtures. Indeed, AMPA was the only compound detected in one groundwater well used for drinking, at values bellow EQSGw/DWQS. Such monitoring results were not expected as the modelling estimations under local pedoclimatic conditions indicated no risk of leaching to groundwater. However, the modelling performance and output may have been influenced by the non-consideration of important specific processes. Integrating monitoring and modelling results with information concerning the agricultural practices adopted and the wells use and location, possible contamination drivers were identified. These include the non-agricultural use of glyphosate in the farmyard, the point source contamination of wells and the possible transport with the subsurface lateral inflow of water from up-hill vineyard. This study strengthens the position of SETAC EMAG-Pest GW group concerning the necessity of spatial and temporal contextualisation of groundwater monitoring for a better understanding of its contamination drivers by PPPs.

Setting the sustainable development targets for restaurants and Italian HoReCa sector.

Due to its strategic position and direct relationship with multiple stakeholders, the HoReCa sector can be a crucial node for the promotion of sustainability in the agri-food chain. However, the sector is currently responsible for a high environmental, social, and economic load. A recent response to these impacts is the diffusion of initiatives that assess and promote sustainable practices at the catering business level. The study aims to verify, using a scientific methodology, how current sustainability programs enable the HoReCa sector to achieve the Sustainable Development Goals (SDGs) and the level of implementation of sustainable practices in the Italian context. To this end, seven currently available national and international sustainability programs were selected, analyzed, and compared. A matrix approach was adopted to understand the balance of the programs with respect to the three pillars of sustainability. A more in-depth analysis was conducted on the Italian Ristorazione Sostenibile 360 (RS 360) case study, which was analyzed both in terms of compliance with the SDGs and in terms of the sustainability performance of the 29 restaurants that participated in the pilot phase of the project. Although the programs differ from each other, they comply with the 2030 Agenda goals, with a particular focus on achieving Goal 12. Furthermore, the analysis highlighted how a balance between the social, environmental, and economic spheres is essential to ensure the sustainable growth of the sector and to bring benefits to the community. As for the case study, 76 % of the analyzed restaurants achieve at least 70 % of the maximum level of fulfillment of the SDGs achievable by the HoReCa sector. This is a symptom of the sector's potential to contribute to supply chain sustainability. However, continuous improvement is essential to ensure the resilience of the HoReCa sector in the future.

Perceptions and Liking Distortion from Information about the Nutritional Upgrades in Biofortified Seafood Products.

(1) Background: As biofortified fish meat is becoming increasingly available, the use of supplements within fish feed may impact consumers' perceptions and their willingness to pay (WTP) for the product. This study focused on evaluating the sensory liking of, and WTP for, fish produced with fortified feed while understanding the role played by the acquired information on fish fortification. (2) Methods: Hedonic ratings and WTP were measured in an experimental bid. The participants (n = 91) were asked to rate pleasantness and WTP during two different rounds: (i) appearance-information-tasting and (ii) appearance-tasting-information. A total of three fish species (carp, seabream, and trout) were presented to the consumers as being either fortified (with iodine, selenium, and omega-3 fatty acids) or conventional products. (3) Results: For pleasantness, no significant differences were found between the fortified and conventional fish. In contrast, substantial differences emerged when information regarding the products was provided. Providing the relevant information before tasting affected how much the consumers liked the conventional fish, resulting in a preference for it over the fortified fish. Additionally, consumers are willing to pay more for fortified fish, especially when information with respect to fortification is available. Nevertheless, when information about fortification was provided before tasting, the consumer's expectations were not fulfilled. (4) Conclusions: The outcomes of this study clearly indicate that the presence of relevant information impacts how much people like fortified versus conventional fish, as well as their WTP.

MIMERA: An online tool for the sustainable pesticide use at field scale.

As echoed in the European Green Deal and Farm to Fork strategy, the EU agricultural policy has clear objectives of improving the sustainability (environmental, economic and social) of farm productions. The environmental goals are supported by promotion of organic farming and the responsible management of inputs such as fertilisers and pesticides. In this framework, there is a need is to provide farmers with tools to evaluate the best options for reducing environmental impact and measure performances in improving the sustainability of their productions. This paper introduces MIMERA, a GIS easy-to-use tool for the sustainable use of pesticides at farm level. MIMERA was developed in the framework of the Train-Agro project funded by the Lombardia Region (Italy), in the framework of the European Agricultural Fund for Rural Development Programme (EAFRD). The main goal is to help professional pesticide users to identify the best pest management strategies by comparing the environmental risk of authorized commercial formulations of pesticides, identifying parcel most at risk within the farm boundaries and suggesting the most appropriate risk mitigation measures for the prevention of surface water contamination. Finally, MIMERA offers the opportunity to track the risk trend of the selected pest management strategies utilized during years. The proposed methodology and structure of MIMERA are described along with underlying data and algorithms are described. An example of its application by considering different pest management strategies adopted by farmers in an area characterized by the intensive presence of maize crop in Lombardia Region is presented.

Development of a sustainability technical guide for the Italian olive oil supply chain.

Italian olive growing must aim at the transition to economically and environmentally sustainable management systems, linked to premium quality production and to a recognized and remunerated context of biodiversity conservation in compliance with the provisions of the European Union New Green Deal and United Nations Agenda 2030. To assist and facilitate companies in this step, a sustainability technical guide for the Italian olive oil supply chain has been developed, with reference to the four pillars of sustainability. The guide, consisting of 42 requirements, was submitted to 18 olive farms from 8 different Italian regions participating in this pilot study, to assess their level of total sustainability and to receive feedbacks throughout the drafting process. Taken as a whole, the companies have proved to be virtuous in meeting the requirements provided, with percentages of compliance ranging from 86 to 96% according to pillar and from 70 to 100% according to company and showed a remarkable spirit of collaboration and involvement in the construction of the guide. In this regard, the text is aimed to represent a participatory standard for Italian institutions and for other olive countries.

A multi-criteria approach to evaluate the sustainability performances of wines: the Italian red wine case study.

The wine industry has faced two significant environmental problems in recent years: productivity is challenged by environmental trends such as global warming, and buyers are becoming more environmentally conscious. From an environmental standpoint, the food industry is one of the most impacting sectors and wine results as one of the most studied agri-food products in the scientific literature. In general, comprehensive studies that consider an application of set of indicators to evaluate the overall sustainability of wine sector are lacking in literature. This paper aims to carry out a sustainable assessment using different indicators for fifteen Italian red wines: Water Footprint (WF), Carbon Footprint (CF), Vineyard Indicator (VI), and Territory Indicator (TI). VI is an indicator of the vineyard's agronomic management's sustainability at plot level with values ranging from 0 (fully sustainable) to 1 (fully not sustainable), while TI covers the socio-economical aspects of sustainability. Considering system boundaries from cradle to grave, at 90% confidence interval, CF results ranged between 0.97 kg CO2 eq./functional unit and 1.97 kg CO2 eq./functional unit, with an average estimated at 1.47 kg CO2 eq./functional unit, while the WF of a 0.75 L bottle of wine from cradle to gate is 666.7 L/functional unit on average, out of which 86.75% is green, 1.92% is blue and 11.34% is grey water. Concerning the VI, at 90% confidence interval VI results were between 0.117 and 0.498 with an average estimated at 0.307. The results of the correlation analyses confirmed that each indicator is not statistically correlated with each other. Concerning the sub-indicators, a positive correlation has been found between the total CF and the sum of blue and grey WF. The application of a multi-criteria analysis for sustainability performances evaluation of the wine sector presented in this study can be used by wine companies' experts to better assess sustainability performances.

Assessing consumers' attitudes, expectations and intentions towards health and sustainability regarding seafood consumption in Italy.

The EU and its Member States have articulated a sustainability vision 'to live well within the limits of our planet' by 2050. In this context, consumers play a key role, being able to drive seafood production sustainability and responsibility according to their behaviour, also in relation to their attitudes towards health, nutrition and well-being. On the basis of these premises, this research explores Italian consumers' attitudes towards health and sustainability in relation to seafood, in order to segment different target of consumers. The framework used in this study is mainly focused on a quantitative exploratory data collection based on an online survey. Three groups of consumers were identified based on general health interest, perceived benefits of eating seafoods and attitude towards seafoods: Health seekers who eat seafood for duty; Health seekers and seafood lovers; Low commitment to health and indifferent to seafood. Differences among groups related to socio-demographic characteristics, sustainability attitudes, intentions and interest in information about seafood products were also investigated. In particular, the first two groups are more familiar with sustainable seafood products and more interested in information on these products than the third, both in terms of product origin and seasonality. Consumers belonging to second group show a higher probability to buy seafood products considering this characteristic than the other two groups. Based on the results obtained, a strategic plan could be developed to achieve relevant goals in education, communication and sustainability labelling related with seafood products. Following a preliminary scouting carried out with all the seafood stakeholders, a specific territory to test this approach has been already identified in Torre del Cerrano (Italy). These results have a strong implication for policy makers and educational institutions as they identify differences in attitudes and perceptions among consumers that are crucial in order to design the right communication strategy strategies as well as messages content.

Sustainability Certification, a New Path of Value Creation in the Olive Oil Sector: The ITALIAN Case Study.

The Italian extra virgin olive oil supply chain has considerable potential for embarking on a path of sustainable development and evolution. In Italy, the great variety heritage and the different pedo-climatic characteristics result in local olive growing systems with different management techniques, producing extra virgin olive oils that are strictly entwined to the territory, with peculiar qualitative properties. Nevertheless, numerous criticalities have been traditionally eroding the competitiveness of Italian olive growing that could find in sustainability certifications, a lasting driver of value creation. Shared standardizations and certifications that include the three pillars of sustainability are therefore necessary for the development of the process.

Benchmarking of carbon footprint data from the Italian wine sector: A comprehensive and extended analysis.

The interest in sustainability, within the wine sector, is growing simultaneously with the awareness of the environmental impacts on climate change generated by the sector itself. In this context, environmental methodologies need to be applied: Carbon Footprint of a Product (CFP) is a quantitative expression of Greenhouse Gases (GHGs) emissions that plays an influent role in emission management and evaluation of mitigation measures over the full life cycle of a product. Moreover, CFP application in the agri-food sector remains scarce due to complex, expensive, and difficult data collection. This paper aims to determine the main factors that contribute to the CFP of 33 Italian wines from 16 wineries and compare and evaluate the results obtained using all the inventory data or results obtained using a simplified model with fewer inputs. The results per Function Unit (0.75/ L of wine) have been obtained using a unique methodology. Considering system boundaries from cradle to grave, at 90% confidence interval, CFP results ranged between 0.899 kg CO2 eq./FU and 1.882 kg CO2 eq./FU. The study underlines that most of the impacts can be related to few inventory data, in fact the main contributors of GHGs emissions are: glass bottle (29%), electricity used in the winery stage (14%), transport and distribution of the final product (13%), heat used in the winery phase (9%) and fossil fuels used in vineyard (8%). The results can be helpful to support the development of a simplified CFP and to obtain a benchmark for the CFP of the Italian wine sector. Furthermore, the present study can help businesses, policy makers and consumers in making decisions that lead to a better environmental outcome.

Influence of nitrogen-based fertilization on nitrates occurrence in groundwater of hilly vineyards.

Nitrogen losses from intensive agricultural production may end up as high nitrate (NO3-) concentrations in groundwater, with a long-term impact on groundwater quality. The main objective of this study was to evaluate the impact of fertilization practices used for grape cultivation on groundwater quality of Tidone Valley, northwest of Italy, following an integrated socio-hydrogeological approach that consists on (i) the involvement of 175 farmers in the description of agricultural and fertilization practices, using a survey of ad hoc questionnaires, (ii) the evaluation of NO3- occurrence in groundwater and (iii) the identification of NO3- sources through isotopic and hydrochemical analysis. In this area, as for certain particular Apennines shallow aquifers, groundwater is of reduced interest due to its limited storage capacity and there are insufficient wells currently monitored by the local Environmental Agency (ARPAE) to evaluate the impact of agricultural fertilization on existing local aquifers. Farmers' questionnaires results highlighted an extensive use of inorganic nitrogen fertilization and a tendency of farmers to follow their own experience for fertilization. Chemical analyses revealed high variability of major and trace elements concentrations isotope data. NO3- concentrations were significantly higher in deeper wells with respects to shallow wells. Isotopic results indicated that groundwater NO3- origin is inorganic, in agreement with the land use and the declared viticultural practices. Comparing groundwater NO3- occurrence from the studied area with values of entire Emilia-Romagna Region, only 7.7% of groundwater samples showed values above the EQS. (50 mg NO3-/L) between Nov 2017 and Sept 2018, while in the entire region 11.5% of groundwater samples showed values above the EQS in the same period. Considering that the vineyards surface in the studied area represents almost 75% of the entire regional vineyard surface, the obtained results suggest a low to moderate impact of viticulture on NO3-concentration of regional groundwater.

Vehicle-derived ultrafine particulate contaminating bees and bee products.

Despite adverse health effects, ultrafine particulate matter (UFP), i.e., PM less than 0.1 µm in diameter, is an emerging pollutant not subject to regulation. UFP may cause both lung inflammation and cardiopulmonary disease and may enter the brain directly via the olfactory bulb, affecting the nervous system. In highly urbanized environments, diesel and gasoline vehicles are among the major sources of UFP including combustion-generated solid particle pollutant and metal-based particles. Metal-based UFP are of much concern, as they may promote inflammation and DNA damage via oxidative stress with generation of free radicals and reactive oxygen species (ROS). We used the honeybee as an alternative sampling system of UFP in an area of the Po Valley (Northern Italy), which is subject to intense traffic. Worker bees are widely recognised as efficient samplers of air pollutants, including airborne PM. During flight and foraging activity, pubescence of the bees promotes the accumulation of electrical charge on the body's surface, enhancing attraction to air pollutants. Bees living near the main Italian highway, the Autostrada A1, displayed a contamination of nanosized Fe-oxides/hydroxides and baryte. Sources of Fe-bearing and baryte ultrafine particles are primarily the vehicles speeding on the motorway. Pollen collected by forager bees and honey produced by the bee colony displayed contamination by nanosized Fe-oxides/hydroxides and baryte. Such a contamination exposes pollinators and humans to UFP ingestion, endangering the safety of food produced at traffic-influenced sites. Given the global spread of traffic, our findings suggest that exposure and environmental impact of ultrafine Fe-oxides/hydroxides and baryte are potentially ubiquitous, although usually overlooked in environmental policy discussions.

Multi-actor approach and engagement strategy to promote the adoption of best management practices and a sustainable use of pesticides for groundwater quality improvement in hilly vineyards.

The adoption of pesticide mitigation measures and innovation at farm level, are seen as a drivers to reach the sustainable water policy objectives. With the aim to prevent the pesticide pressure of hilly vineyards on groundwater contamination, a stepwise approach in Tidone Valley was applied using different consultation mechanisms and involvement strategies throughout the overall process. Face to face meetings, direct surveys, participatory monitoring and planning of several activities aiming to inform, educate, improve skills, change of individual behaviour or raise awareness, or even initiatives to build institutional trust or support for new investment in innovation are some examples. These activities allowed us to involve key actors of water use and governance (such as farmers, advisors, representatives of drinking water management, farmer's associations, Winemaking cooperatives, local Health Authority), and to have a deeper knowledge of the context agricultural practices, of farmer's knowledge and skills concerning factors influencing water contamination and also to promote the most suitable Best Management Practices aimed at limiting the pesticide occurrence in groundwater. Indeed, the surveys results highlighted that the majority of the farms are small (64% of vineyards <10 ha), that most of the farmers (62%) are not aware of the current legislation on water, even if 64% of them declare to participate regularly to training courses for the prevention of water contamination and that there is a low to moderate level of adoption of Best Practices able to prevent contamination by pesticides. At the end of the overall process, it can be stated that the multi-actor approach and engagement strategy adopted were successful in improving attitudes to more sustainable practices. This is supported also by the monitoring data that show in 2019 a decrease by 44% of pesticides occurrences and a fall by 68% of values above EQSgw if compared with the period 2017-2018.

Development and implementation of a qualitative framework for the sustainable management of wine companies.

A growing number of companies around the world have already adopted sustainable practices as the majority of consumers are increasingly concerned with environmental and health issues. Despite this, lack of knowledge in sustainability can represent a limit to a sustainable conversion of Italian wine companies. This paper focuses on the development and suitability assessment of TERRITORY, a qualitative framework that assists sustainable management of winery farms with respect to biodiversity, neighbouring land use, agrochemical use, soil management, water management, landscape, site selection, heritage-cultural and aesthetic aspects, product quality and safety, human resource management, health of employees, community and consumers, economic repercussion of structures and territories, resource depletion, procurement practices and traceability. 47 wine companies have adopted the framework and its suitability has been tested and validated in a ten years trial. The present work reports the results of TERRITORY framework adoption in a winemaking cooperative, a grape and wine producer and a bottling firm, that respectively performed 76%, 92%, 62% (where 100% is maximum implementation of sustainable practices), with lower adherence in water, landscape and human resources management. The framework has driven wine companies towards improvements implementation: most relevant deal with a more effective communication to local community and consumers about health and safety, protection of biodiversity (towards a deeper knowledge of its territorial features), territorial values enforcement and improved communication. From the results it emerges that the framework guides the structural transformation of wine farms towards sustainable production and management by assisting the wine producers in identifying emerging opportunities in wine sector, such as fostering network with local communities, defining better soil, and water management through a multi-actor approach. Furthermore, it has been adopted in addressing the selection of quantitative indicators to adopt and monitoring campaigns.

A multi-actor, participatory approach to identify policy and technical barriers to better farming practices that protect our drinking water sources.

Despite an improvement of water quality across Europe there are many pollution hotspots for both nitrates and PPPs, mainly due to agricultural activities. The BMPs and MMs to reduce pollution from agriculture are well known, and there are policy instruments in place to ensure drinking water standards, but the current approach has not been efficient enough. Within the H2020 Water Protect project the premise was that there is a need for a multi-actor, participatory approach to tackle the issue from a new angle, and to assess why the uptake of known BMPs and MMs was not better among farmers. Seven "Action Labs" were selected that represent major physical, socio-economical, cultural and farming settings across Europe. A methodology of multi-actor engagement was chosen but with different approaches due to the local context. Initially the level of farmers' awareness about water quality issues was matched to the observed uptake rates of BMPs and MMs. In a second survey barriers hindering the uptake of measures were identified. The first survey revealed a low general awareness on the potential pollution to drinking water sources. Despite this, between 24% to 88% of the surveyed farmers per Action Lab were already voluntarily adopting one quarter of the selected BMPs and MMs. The second survey demonstrated the need to address organisational, legislative, sociological and technical barriers. The lack of coordination between different institutional bodies promoting measures and the financial incentives needed to invest and operate these often-costly measures need to be considered. The multi-actor, participatory approach with its improved awareness and collaboration made it possible to identify the crucial factors for improvement - to build a social acceptance among all actors and communicate the issues and solutions from the start.

Evaluation of groundwater contamination sources by plant protection products in hilly vineyards of Northern Italy.

In Europe, 25% of groundwater has poor chemical status. One of the main stressors is agriculture, with nitrates and plant protection products (PPPs) causing failure in 18% and 6.5%, respectively, of groundwater bodies (by area). EU legislation for the placement of the PPPs on the market is one of the most stringent in the world. However, recent monitoring studies in hilly vineyards of Tidone Valley, north-west of Italy, show presence of PPPs used for grapevine cultivation in 15 out of 26 groundwater wells monitored, at values above the Environment Quality Standard (EQS) for groundwater (0.1 µg/L). However, no information about the contamination sources are available. Therefore, the objective of the present work is to evaluate the groundwater contamination sources by PPPs, in a small catchment with intensive viticulture, by collecting and integrating monitoring data, sub-surface water movement data and territorial characteristics. The results show that in wells used for PPP's mixture preparation and sprayer washing located at the top of hilly vineyards, with low slope and no water movement in the surrounding soil, the contamination is most likely from point sources. On the contrary, for wells located in a fenced area at the bottom of the hill, far away from vineyards and being used for drinking water production, the contamination is most likely from diffuse sources. Our results were used to raise awareness on groundwater contamination from PPPs among farmers in the study area; moreover a waterproof platform for sprayers washing, equipped with wastewater recovery and disposal system, able to avoid point-source contamination, was implemented in a local demonstration farm. Several demonstration activities were then organised with the farmers of the entire Valley in order to show its functionality and promote its diffuse use.

First evaluation of pesticides occurrence in groundwater of Tidone Valley, an area with intensive viticulture.

Agricultural practice often involves an intensive and incorrect use of pesticides and fertilizers. These chemicals can leach through the soil profile and contaminate groundwater, including drinking water. For this reason, an effective groundwater monitoring is strongly advisable. The aim of this study was to investigate the groundwater contamination by plant protection products (PPPs) on a hilly area situated in the Tidone Valley, North-West of Italy, a region characterized by an intensive viticulture production. This area is not included in the national groundwater monitoring plan and therefore scarce information is available regarding the quality of groundwater, even though the local Environmental Agency previously revealed the occurrence of PPPs at values higher than the Environmental Quality Standard downstream this area. Hence, a monitoring wells network was developed following an upstream-downstream criterion, a list of pesticides to be monitored, based on a multi-actor approach, and an analytical method for PPPs detection and quantification. The analytical approach involved solid phase extraction followed by High-Performance Liquid Chromatography tandem mass spectrometry. The results of three monitoring campaigns revealed the occurrence of seven PPPs at a level higher than EQS for groundwater (0.1 µg/L) in 30% of the wells. The main pesticides detected were Chlorantraniliprole, Dimethomorph, Fluopicolide, Metalaxyl-M, Penconazole, and Tetraconazole, all commonly used in viticulture, together with S-metolachlor, authorized for cereal cropping. Statistical analysis revealed a significant influence of the sampling time, slope of the soil surrounding the wells, wells depth and wells location on the concentration of five PPPs. Therefore, the results obtained show that the improper use of PPPs for grapevine cultivation may cause groundwater contamination and suggest the need for a deeper analysis of territorial reality, including hydrology studies and farmer behavior and for an urgent introduction of best management practices and mitigation measures to promote a sustainable use of PPPs in viticulture.

Investigating combined toxicity of binary mixtures in bees: Meta-analysis of laboratory tests, modelling, mechanistic basis and implications for risk assessment.

Bees are exposed to a wide range of multiple chemicals "chemical mixtures" from anthropogenic (e.g. plant protection products or veterinary products) or natural origin (e.g. mycotoxins, plant toxins). Quantifying the relative impact of multiple chemicals on bee health compared with other environmental stressors (e.g. varroa, viruses, and nutrition) has been identified as a priority to support the development of holistic risk assessment methods. Here, extensive literature searches and data collection of available laboratory studies on combined toxicity data for binary mixtures of pesticides and non-chemical stressors has been performed for honey bees (Apis mellifera), wild bees (Bombus spp.) and solitary bee species (Osmia spp.). From 957 screened publications, 14 publications provided 218 binary mixture toxicity data mostly for acute mortality (lethal dose: LD50) after contact exposure (61%), with fewer studies reporting chronic oral toxicity (20%) and acute oral LC50 values (19%). From the data collection, available dose response data for 92 binary mixtures were modelled using a Toxic Unit (TU) approach and the MIXTOX modelling tool to test assumptions of combined toxicity i.e. concentration addition (CA), and interactions (i.e. synergism, antagonism). The magnitude of interactions was quantified as the Model Deviation Ratio (MDR). The CA model applied to 17% of cases while synergism and antagonism were observed for 72% (MDR > 1.25) and 11% (MDR < 0.83) respectively. Most synergistic effects (55%) were observed as interactions between sterol-biosynthesis-inhibiting (SBI) fungicides and insecticide/acaricide. The mechanisms behind such synergistic effects of binary mixtures in bees are known to involve direct cytochrome P450 (CYP) inhibition, resulting in an increase in internal dose and toxicity of the binary mixture. Moreover, bees are known to have the lowest number of CYP copies and other detoxification enzymes in the insect kingdom. In the light of these findings, occurrence of these binary mixtures in relevant crops (frequency and concentrations) would need to be investigated. Addressing this exposure dimension remains critical to characterise the likelihood and plausibility of such interactions to occur under field realistic conditions. Finally, data gaps and further work for the development of risk assessment methods to assess multiple stressors in bees including chemicals and non-chemical stressors in bees are discussed.

Residents perceptions of non-dietary pesticide exposure risk. Knowledge gaps and challenges for targeted awareness-raising material in Italy.

Currently there are no tools to accurately estimate pesticides exposure risk for residents and bystanders. European Member States have to develop specific measures and communication strategies to prevent and minimize non-occupational pesticides exposure. Moreover, these measures should be compliant with the requirements of the Directive on Sustainable Use of Pesticides. Unfortunately, there is a high degree of uncertainties in the assessment of the non-dietary exposure risk for residents, therefore risk communication passes through a deep understanding of exposure risk perception. The objective of this pilot study is to assess citizens' risk perception of non-dietary exposure to pesticides, and to assist policy-makers and risk communicators in developing targeted awareness-raising materials for residents and bystanders. Through a household survey, conducted in the rural area of the province of Piacenza (IT) we investigated knowledge, health risk perceptions, and information sources related to non-dietary exposure to agricultural pesticides in residents' indoor and outdoor environment. The factors that push individuals to give importance to several possible pollution sources and to mitigation measures or precaution, in order to protect themselves from possible exposure sources, were also investigated. Results show that even if the air quality of the residential area is not judged negatively, pesticides are perceived as air pollutants that could lead to an actual exposure and, are correlated to the health status. The perception of risk, however, does not seem to be dependent only on the distance between homes and fields. The interpretative hypothesis that the perception of the relationship between air quality and health is influenced by the cultural issue and by psycho-sensory factors and not supported by proper information, even if with some differences among age groups, it seems to be confirmed. To better transfer knowledge and communication, the commitment of those who are recognised as "competent" (doctors and researchers) is critical.

Levels of regulated POPs in fish samples from the Sava River Basin. Comparison to legislated quality standard values.

Fish samples of different species (i.e. rainbow trout (Onchorhynchus mykiss), barbel (Barbus barbus) and European chub (Squalius cephalus)) were collected from the Sava River Basin for a preliminary investigation of the levels of PCDD/Fs, PCBs, PBDEs and PFAS as a whole. Concentrations of PCDD/Fs, in terms of pg WHO-TEQ/g ww, were below the maximum limit established at the Commission Regulation (EU) No 1259/2011. On the contrary, when DL-PCBs were also included, levels increase up to 11.7 pg WHO-TEQPCDD/Fs+DL-PCBs/g ww in a particular case, with two samples out of a total of ten exceeding the maximum set at this EU Regulation and the EQS established at the European Directive regarding priority substances in the field of water policy (0.0065 ng WHO-TEQPCDD/Fs+DL-PCBs/g ww). A similar trend was also observed for NDL-PCBs, whit the same two samples, from the lower stretch of the river basin, exceeding the maximum limit allowed at the EU Regulation (125 ng/g ww). For PBDEs, levels found in all the samples exceeded the EQS (0.0085 ng/g ww) up to more than a thousand times and 40% of the samples presented PFOS values above the EQS. Data from this study were compared to values reported at the literature for fish from other geographical areas.