Effectiveness of periprostatic block to prevent pain in transrectal prostate biopsy: a systematic review and a network meta-analysis.

INTRODUCTION: The purpose of this study was to determine the effectiveness and harms of periprostatic block compared with other interventions in patients with clinically suspected prostate cancer who underwent transrectal biopsy to diminish pain. MATERIAL AND METHODS: We included only clinical trials which involved male adults older than 18 years-old suspected of having prostate cancer. The intervention performed was a periprostatic block and the comparators were topical anesthetics, sedatives, placebo/no intervention or combined therapies. The primary outcome was perianal or perineal pain and serious adverse effects (SAE). Literature search was conducted in MEDLINE, EMBASE, LILACS, CENTRAL and non-published literature from inception to March 2019. We performed a network meta-analysis in R. RESULTS: We included 43 studies in the meta-analysis. Thirteen studies compared periprostatic block vs. placebo/no intervention (the most frequent). Most of the studies had an unclear risk of bias for selection, performance and detection bias and low risk for attrition, reporting and other bias. Periprostatic block (lidocaine) + intrarectal gel (lidocaine + prilocaine) vs. periprostatic block (lidocaine) showed an RR -0.9 (95%CI - 1.9 to 0.074); intrarectal gel (lidocaine) vs. periprostatic block (lidocaine) had a RR 0.77 (95%CI 0.14 to 1.4); placebo/no intervention vs. periprostatic block (lidocaine) + intrarectal gel (lidocaine+prilocaine) RR 3 (95%CI 1.9 to 4); intrarectal gel (lidocaine) versus periprostatic block (lidocaine) + intrarectal gel (lidocaine + prilocaine) RR 1.7 (95%CI 0.64 to 2.7). CONCLUSIONS: The blockage of the periprostatic plexus in the performance of a transrectal ultrasound-guided prostatic biopsy, alone or in combination with intrarectal analgesia or sedation, is an effective method to reduce pain.

On the origin of pure optical rotation in twisted-cross metamaterials.

We present an experimental and computational study of the response of twisted-cross metamaterials that provide near dispersionless optical rotation across a broad band of frequencies from 19 GHz to 37 GHz. We compare two distinct geometries: firstly, a bilayer structure comprised of arrays of metallic crosses where the crosses in the second layer are twisted about the layer normal; and secondly where the second layer is replaced by the complementary to the original, i.e. an array of cross-shaped holes. Through numerical modelling we determine the origin of rotatory effects in these two structures. In both, pure optical rotation occurs in a frequency band between two transmission minima, where alignment of electric and magnetic dipole moments occurs. In the cross/cross metamaterial, the transmission minima occur at the symmetric and antisymmetric resonances of the coupled crosses. By contrast, in the cross/complementary-cross structure the transmission minima are associated with the dipole and quadrupole modes of the cross, the frequencies of which appear intrinsic to the cross layer alone. Hence the bandwidth of optical rotation is found to be relatively independent of layer separation.

Incidencia, mortalidad y supervivencia al cáncer de próstata en Cali, Colombia, 1962-2011 / Prostate cancer in Cali, Colombia, 1962-2011: incidence, mortality and survival

Objetivo. Analizar la tendencia de las tasas de incidencia, mortalidad y supervivencia al cáncer de próstata (CP) en el periodo 1962-2011. Material y métodos. Con base en el Registro Poblacional de Cáncer en Cali (Colombia) y los registros de mortalidad de la Secretaría de Salud Pública Municipal se analizaron las tendencias de las tasas de incidencia, mortalidad y supervivencia por CP en Cali durante el periodo 1962-2011. Resultados. La incidencia de cáncer de próstata aumentó vertiginosamente entre 1986 y 2002 (Annual Percent Change APC 6.2%) y se estabilizó posteriormente. La mortalidad disminuyó desde 1997 en los mayores de 70 años, mientras que en el grupo de 50-69 años fue a partir de 1981. La supervivencia relativa a cinco años fue 69.8% (IC95% 67.5-72.0) y se asoció de manera significativa con la edad, periodo de diagnóstico y estrato socioeconómico. Conclusión. El incremento en la incidencia del CP coincide temporalmente con la implementación del antígeno específico de próstata (PSA por sus siglas en inglés) en Cali; hay evidencia de mejoría en la supervivencia en el CP y disminución en su mortalidad.

Objective. To analyze the trend in prostate cancer survival, incidence and mortality rates in Cali, Colombia from 1962 to 2011. Materials and methods. Based on the Cancer Registry of Cali, Colombia and the mortality registry of the City's Public Health Secretary, incidence, mortality age-standardized rates and relative survival were calculated during 1962-2011. Results. Prostate cancer incidence rates increased sharply between 1986 and 2002 (APC: 6.21%) and then leveled off. Mortality diminished in 1997 in men older than 70 years-old while in men aged 50-69 years declined since 1981. The 5-year-relative-survival was 69.8% (CI95% 67.5-72.0) and it was significantly associated with age, quinquennial period of diagnosis and socioeconomic strata. Conclusion. The increase in incidence rates of prostate cancer in time coincides with the implementation of the PSA in Cali. There is evidence of improvement in prostate cancer survival, and decreased prostate cancer mortality.

[Prostate cancer in Cali, Colombia, 1962-2011: incidence, mortality and survival]. / Incidencia, mortalidad y supervivencia al cáncer de próstata en Cali, Colombia, 1962-2011.

OBJECTIVE: To analyze the trend in prostate cancer survival, incidence and mortality rates in Cali, Colombia from 1962 to 2011. MATERIALS AND METHODS: Based on the Cancer Registry of Cali, Colombia and the mortality registry of the City's Public Health Secretary, incidence, mortality age-standardized rates and relative survival were calculated during 1962-2011. RESULTS: Prostate cancer incidence rates increased sharply between 1986 and 2002 (APC: 6.21%) and then leveled off. Mortality diminished in 1997 in men older than 70 years-old while in men aged 50-69 years declined since 1981. The 5-year-relative-survival was 69.8% (CI95% 67.5-72.0) and it was significantly associated with age, quinquennial period of diagnosis and socioeconomic strata. CONCLUSION: The increase in incidence rates of prostate cancer in time coincides with the implementation of the PSA in Cali. There is evidence of improvement in prostate cancer survival, and decreased prostate cancer mortality.

Efficacy of antibiotic prophylaxis in patients undergoing cystoscopy: a randomized clinical trial.

OBJECTIVE: To establish the efficacy of antibiotic prophylaxis prior to cystoscopy in outpatients in decreasing the incidence of post-procedure urinary tract infection. STUDY DESIGN AND SETTING: A randomized clinical trial in patients (men and women) older than 18 who underwent cystoscopy for any non-urgent indication. The intervention was Levofloxacin 500 mg single dose, and the control was placebo 500 mg single dose made with similar characteristics. The primary outcome was urinary tract infection (UTI) measured 3-10 days after the procedure. It was performed as per protocol analysis. RESULTS: Hundred and thirty-eight patients in each study arm completed the trial. The incidence of UTI in the intervention group was 0.7% and in the placebo group was 3% (p = 0.17), and no significant differences were found. The incidence of asymptomatic bacteriuria in the intervention group was 5.8% and in the control group was 14.5% (p = 0.01). CONCLUSIONS: No significant differences were found in the use of prophylactic antibiotic compared to placebo to reduce the incidence of UTI in patients who undergo cystoscopy as an outpatient procedure with sterile urine demonstrated by urine culture.

Transperitoneal laparoscopic management of urinary tract stone disease: experience in a third level hospital.

OBJECTIVES: The aim of this paper is to describe the clinical-surgical characteristics of patients who underwent laparoscopic procedures for the management of urinary tract stone disease when performing extracorporeal, percutaneous or endourological procedures was not available. METHODS: A descriptive study based on information from the medical records of patients who underwent surgical laparoscopic management of urinary stone disease between January 2001 and May 2010 at a third level hospital. Epidemiological, clinical and procedure-related variables were taken from the medical records. Univariate analysis was performed with the statistical software STATA 10.1. RESULTS: There were 29 procedures (27 patients) for treatment of urinary stone disease in adults. The average age was 45 years. 55% of patients were men. 17 stones were found on the right side, 5 were pyelic, 19 of the proximal ureter, 4 of the medium ureter and 1 distal. All patients underwent laparoscopic surgery as first surgical option. Average operative time was 142 ± 32 minutes. Three procedures were defined as failures. Hospital stay presented a median of 2 days. There were seven complications. CONCLUSION: Laparoscopic surgery is a good surgical option for the management of urinary tract stone disease in adults.

Evaluación de la necrosis de Fournier en un hospital de alta complejidad / Evaluation of Fournier`s necrosis in a high complexity hospital

OBJETIVO: Determinar las características clínicas de la población con Necrosis de Fournier que consulto al Hospital Universitario del Valle (HUV) en Cali durante el período 2003-2008.MÉTODOS: Estudio descriptivo. Se realizo una revisión retrospectiva de los pacientes atendidos por Necrosis de Fournier en el H.U.V durante el período 2003-2008. Se recopilo información sobre la edad, antecedentes personales, tiempo de evolución de la enfermedad, estancia hospitalaria, cirugías realizadas, tiempo hasta el tratamiento quirúrgico, gérmenes aislados y mortalidad. Análisis descriptivo univariado en STATA v 10.1RESULTADOS: 42 pacientes con edad promedio de 51 anos y tiempo de evolución promedio de 12 días. 26% tenían antecedente de diabetes mellitus y 21.4% traumatismo uretral previo al ingreso. El tiempo promedio hasta el desbridamiento quirúrgico fue de 41.4 hrs. 62% requirieron cistotomía suprapúbica, 14.3% colostomía derivativa, 9.5% orquidectomía y 2.4% penectomia. El promedio de estancia hospitalaria fue de 23 días y 12% requirió manejo en UCI.59.5% de los pacientes se le aisló algún germen en escroto de los cuales 64% un único germen y 36% fueron polimicrobiano. La mortalidad reportada fue de 17%.CONCLUSIÓN: La necrosis de fournier es una entidad clínica que amenaza la vida en pacientes con múltiples comorbilidades y con requerimiento de múltiples intervenciones. El manejo antibiótico de amplio espectro, el debridamiento agresivo del tejido necrótico y el manejo integral de estos pacientes son los pilares fundamentales en el tratamiento de éstos pacientes severamente enfermos(AU)

OBJECTIVES: To determine clinical characteristics of the population with Fournier`s necrosis at Hospital Universitario del Valle (HUV) in Cali during the period 2003-2008.METHODS: We performed a retrospective review of patients with the diagnosis of Fournier´s necrosis at HUV during the period 2003-2008. We collected information on age, personal history, duration of illness, hospitalization, surgeries performed, time to surgical treatment, isolated germs, and mortality. Univariate descriptive analysis was performed in STATA v 10.1. RESULTS: 42 patients with mean age 51 years and 12 days mean disease duration. 26% had diabetes mellitus and 21.4% urethral trauma before admission. Average time to surgical debridement was 41.4 hours. 62% required suprapubic cystostomy, 14.3% derivative colostomy, 9.5% and 2.4% orchiectomy and penectomy respectively. The average hospital stay was 23 days and 12% required ICU care.Scrotal cultures were positive in 59.5%: 64% a single germ and 36% polymicrobial. Reported mortality was 17%.CONCLUSION: Fournier`s necrosis is a life-threatening clinical entity in patients with multiple comorbidities requiring multiple interventions. Broad-spectrum antimicrobial therapy, aggressive debridement of necrotic tissue and comprehensive management of these patients are the mainstays in the treatment of these severely ill patients(AU)

Utilidad de la ecografía vésico-prostática en el diagnóstico de obstrucción/hiperactividad en varones con HBP / Usefulness of bladder-prostate ultrasound in the diagnosis of obstruction/hyperactivity in males with BPH

OBJETIVO: El objetivo principal del presente artículo fue describir las características clínicoquirúrgicas de los pacientes que fueron llevados a procedimientos laparoscópicos como manejo de enfermedad litiásica del tracto urinario cuando no se dispone de equipamiento para la realización de procedimientos extracorpóreos, percutáneos ni endourológicos.MÉTODOS: Se realizó un estudio observacional descriptivo basado en la información de las historias clínicas de los pacientes que fueron llevados a cirugía laparoscópica como manejo de enfermedad litiásica entre enero de 2001 y mayo de 2010 en el Hospital Universitario del Valle. Se tuvieron en cuenta variables epidemiológicas, clínicas y relacionadas a los procedimientos. Se realizó un análisis univariado con el programa estadístico STATA 10.1.RESULTADOS: Se encontraron 29 procedimientos (27 pacientes) para manejo de enfermedad litiásica en adultos. La edad promedio fue 45 años. 55% de los pacientes fueron hombres. 17 cálculos se encontraron al lado derecho y de la totalidad de ellos 5 eran piélicos, 19 proximales, 4 en uréter medio y 1 distal. Todos los pacientes fueron llevados a cirugía laparoscópica como primera opción quirúrgica. La duración promedio del acto quirúrgico fue 142 ± 32 minutos. Tres procedimientos fueron definidos como fallidos. El tiempo de hospitalización presentó una mediana de 2 días. Se presentaron 5 complicaciones.CONCLUSION: La cirugía laparoscópica es una buena opción quirúrgica para manejo de la enfermedad litiásica del tracto urinario en adultos(AU)

OBJECTIVES: The aim of this paper is to describe the clinical-surgical characteristics of patients who underwent laparoscopic procedures for the management of urinary tract stone disease when performing extracorporeal, percutaneous or endourological procedures was not available.METHODS: A descriptive study based on information from the medical records of patients who underwent surgical laparoscopic management of urinary stone disease between January 2001 and May 2010 at a third level hospital. Epidemiological, clinical and procedure-related variables were taken from the medical urirecords.Univariate analysis was performed with the statistical software STATA 10.1.RESULTS: There were 29 procedures (27 patients) for treatment of urinary stone disease in adults. The average age was 45 years. 55% of patients were men. 17 stones were found on the right side, 5 were pyelic, 19 of the proximal ureter, 4 of the medium ureter and 1 distal. All patients underwent laparoscopic surgery as first surgical option. Average operative time was 142 ± 32 minutes. Three procedures were defined as failures. Hospital stay presented a median of 2 days. There were seven complications.CONCLUSION: Laparoscopic surgery is a good surgical option for the management of urinary tract stone disease in adults(AU)

Evaluation of Fournier's necrosis in a high complexity hospital.

OBJECTIVES: To determine clinical characteristics of the population with Fournier's necrosis at Hospital Universitario del Valle (HUV) in Cali during the period 2003-2008. METHODS: We performed a retrospective review of patients with the diagnosis of Fournier's necrosis at HUV during the period 2003-2008. We collected information on age,personal history, duration of illness, hospitalization, surgeries performed, time to surgical treatment, isolated germs, and mortality. Univariate descriptive analysis was performed in STATA v 10.1. RESULTS: 42 patients with mean age 51 years and 12 days mean disease duration. 26% had diabetes mellitus and 21.4% urethral trauma before admission. Average time to surgical debridement was 41.4 hours. 62% required suprapubic cystostomy, 14.3% derivative colostomy, 9.5% and 2.4% orchiectomy and penectomy respectively. The average hospital stay was 23 days and 12% required ICU care. Scrotal cultures were positive in 59.5%: 64% a single germ and 36% polymicrobial. Reported mortality was 17%. CONCLUSION: Fournier's necrosis is a life-threatening clinical entity in patients with multiple comorbidities requiring multiple interventions. Broad-spectrum antimicrobial therapy, aggressive debridement of necrotic tissue and comprehensive management of these patients are the mainstays in the treatment of these severely ill patients.

Clinical experience in the management of penile fractures at Hospital Universitario del Valle (Cali--Colombia).

SUMMARY OBJECTIVES: The main objective of this study was to describe the clinical characteristics of patients diagnosed with penile fracture in the Hospital Universitario del Valle (Cali, Colombia). METHODS: A descriptive study, reviewing all the medical records of patients diagnosed with penile fracture from January 2001 to December 2008 at Hospital Universitario del Valle (.HUV).in Cali. It took into account variables related to urological history, etiology, diagnosis, the surgical treatment and follow-up. Univariate analysis was performed with the statistical program STATA v. 10.1 RESULTS: There were 18 cases of penile fracture with an average age of 30 years. 11 patients (.61%). had episodes related to intercourse. Patients presented swelling, pain and popping or cracking sound. The diagnosis was done by history and physical examination in 100%. Surgery was performed with a subcoronal incision in most of the patients. The right corpus cavernosum was frequently injured and corrected with absorbable suture. The patients had an average of 1.5 days of postoperative hospital stay. CONCLUSIONS: Penile fracture is an entity the diagnosis of which is straightforward and can be reliable by history and physical examination. Surgical repair is the treatment of choice preventing complications, allowing the patient to return to satisfactory sexual life.

Experiencia clínica en el manejo de fracturas de pene en el Hospital Universitario del Valle (Cali-Colombia) / Clinical experience in the management of penile fracture at Hospital Universitario del Valle (Cali-Colombia)

OBJETIVO: El objetivo principal del presente estudio fue describir las características clínicas de los pacientes con diagnóstico de fractura de pene en el Hospital Universitario del Valle (Cali, Colombia).MÉTODOS: Se realizó un estudio descriptivo, en el que se revisaron todas las historias clínicas de los pacientes con diagnóstico de fractura de pene entre enero de 2001 a diciembre de 2008 en el Hospital Universitario del Valle (HUV) en la ciudad de Cali. Se tuvieron en cuenta variables relacionadas con los antecedentes urológicos, la etiología, el diagnóstico de la fractura, el procedimiento quirúrgico y el seguimiento. Se realizó un análisis univariado con el programa estadístico STATA v. 10.1RESULTADOS: Se encontraron 18 casos de fractura de pene con un promedio de edad de 30 años. 11 pacientes (61%) presentaron el episodio secundario a la relación sexual. Se presentó edema, dolor y chasquido más frecuentemente. El diagnóstico fue clínico en el 100% de los casos. El abordaje quirúrgico se realizó con una incisión circunferencial en la mayoría de los pacientes. El cuerpo cavernoso derecho fue el más frecuentemente lesionado y en la mayoría de los casos la lesión fue corregida con una sutura absorbible de forma continua. Los pacientes tuvieron una hospitalización posquirúrgica en promedio de 1.5 días.CONCLUSIONES: La fractura de pene es una entidad cuyo diagnóstico es clínico, debe realizarse de forma precoz y la reparación quirúrgica es el tratamiento de elección para evitar complicaciones y permitir que el paciente retorne a su actividad sexual satisfactoria de manera temprana(AU)

OBJECTIVES: The main objective of this study was to describe the clinical characteristics of patients diagnosed with penile fracture in the Hospital Universitario del Valle (Cali, Colombia).METHODS: A descriptive study, reviewing all the medical records of patientsdiagnosed with penile fracture from January 2001 to December 2008 at Hospital Universitario del Valle (HUV) in Cali. It took into account variables related to urological history, etiology, diagnosis, the surgical treatment and follow-up. Univariate analysis was performed with the statistical program STATA v. 10.1. RESULTS: There were 18 cases of penile fracture with an average age of 30 years. 11 patients (61%) had episodes related to intercourse. Patients presented swelling, pain and popping or cracking sound. The diagnosis was done by history and physical examination in 100%. Surgery was performed with a subcoronal incision in most of the patients. The right corpus cavernosum was frequently injured and corrected with absorbable suture. The patients had an average of 1.5 days of postoperative hospital stay.CONCLUSIONS: Penile fracture is an entity the diagnosis of which is straightforward and can be reliable by history and physical examination. Surgical repair is the treatment of choice preventing complications, allowing the patient to return to satisfactory sexual life(AU)

[Prevalence of abnormal coagulation tests in patients who undergo transrectal biopsy of the prostate]. / Prevalencia de alteración en las pruebas de coagulación en pacientes sometidos a biopsia transrectal de próstata.

PURPOSE: To determine the prevalence of abnormal coagulation tests in patients who undergo transrectal prostate biopsy. MATERIAL AND METHODS: A cross-sectional study was performed at Hospital Universitario del Valle (HUV) between 1 June and 31 December 2008. Variables collected included age, PSA value, PT (prothrombin time), PTT (partial thromboplastin time) and INR (international normalized ratio) values and the presence of abnormalities in each of the haematological variables. There are no real normal or maximum acceptable values in clinical practice; however, the values described by some authors and different clinical practice protocols serve as a guide. RESULTS: The average age was 70 years, the median PSA was 28, the partial thromboplastin time was altered in 2.3% of patients, and INR was abnormal in 3.4% of patients (cut-off point 1.5). There were no changes in PT or INR values (cut-off point 2.0). CONCLUSIONS: The prevalence of abnormal coagulation tests is low even when there are no clear cut-off points in worldwide literature that would determine abnormality.

Prevalencia de alteración en las pruebas de coagulación en pacientes sometidos a biopsia transrectal de próstata / Prevalence of abnormal coagulation tests in patients who undergo transrectal biopsy of the prostate

Objetivo: Determinar la prevalencia de las alteraciones en las pruebas de coagulación en pacientes sometidos a biopsia transrectal de la próstata. Material y métodos: Se realizó un estudio de corte transversal en el hospital universitario del valle (HUV) entre el 1 de junio y el 31 de diciembre de 2008. Se recolectaron variables tales como edad, valor del antígeno prostático específico (PSA), valor del PT (tiempo de protrombina), valor del PTT (tiempo parcial de tromboplastina), valor de INR (relación internacional normalizada) y la presencia de anormalidad en cada una de las variables hematológicas. No existen valores normales o máximos aceptables en realidad en la práctica clínica; sin embargo, se toman como base los valores descritos por algunos autores y diferentes protocolos de práctica clínica. Resultados: El promedio de edad fue 70 años, la mediana del PSA fue 28, el PTT fue alterado en el 2,3%, el INR fue anormal en el 3,4% (punto de corte, 1,5). No se encontraron alteraciones en los valores de PT ni en el INR (punto de corte, 2,0). Conclusiones: La prevalencia de alteraciones en las pruebas de coagulación es baja, aun cuando no existen puntos de corte claros en la literatura científica mundial para determinar anormalidad (AU)

Purpose: To determine the prevalence of abnormal coagulation tests in patients who undergo transrectal prostate biopsy. Material and methods: A cross-sectional study was performed at Hospital Universitario del Valle (HUV) between 1 June and 31 December 2008. Variables collected included age, PSA value, PT (prothrombin time), PTT (partial thromboplastin time) and INR (international normalized ratio) values and the presence of abnormalities in each of the haematological variables. There are no real normal or maximum acceptable values in clinical practice; however, the values described by some authors and different clinical practice protocols serve as a guide. Results: The average age was 70 years, the median PSA was 28, the partial thromboplastin time was altered in 2.3% of patients, and INR was abnormal in 3.4% of patients (cut-off point 1.5). There were no changes in PT or INR values (cut-off point 2.0). Conclusions: The prevalence of abnormal coagulation tests is low even when there are no clear cut-off points in worldwide literature that would determine abnormality (AU)

Método analítico por cromatografía líquida de alta resolución para la determinación de carbamazepina en plasma humano / Analytical method by high resolution liquid chromatography for the determination of carbamazepine in human plasma

Entre los requisitos para desarrollar los estudios de biodisponibilidad y bioequivalencia se encuentra contar con metodologías analíticas validadas para el trabajo con muestras en fluidos biológicos. Se desarrolló un método por cromatografía líquida de alta resolución para la determinación de carbamazepina en plasma humano, se utilizó como fase móvil una mezcla de hidrógeno fosfato de sodio: acetonitrilo (65:35) ajustada a pH= 3,3 con ácido fosfórico, flujo de 1,2 mL/min y detección ultravioleta a 210 nm. Se empleó propilparabeno como estándar interno. Conforme con las regulaciones establecidas para la validación de métodos en fluidos biológicos se estudiaron los parámetros siguientes: estabilidad de las muestras, linealidad, especificidad, precisión, exactitud y límite de detección y cuantificación. El método resultó específico y sensible con un límite de detección y cuantificación de 0,9 y 1,0 ng, respectivamente. El método fue lineal, preciso y exacto en el rango de concentraciones de 1,07 a 12,67 µg/mL. La recuperación media no fue estadísticamente diferente del 100,0 por ciento. El analito en la matriz biológica propuesta permaneció en el periodo estudiado. La metodología descrita en este trabajo se aplica en nuestro caso al estudio que evalúa la biodisponibilidad y bioequivalencia de una formulación cubana de carbamazepina en voluntarios sanos

Método analítico por cromatografía líquida de alta resolución para la determinación de carbamazepina en plasma humano / Analytical method by high resolution liquid chromatography for the determination of carbamazepine in human plasma

Entre los requisitos para desarrollar los estudios de biodisponibilidad y bioequivalencia se encuentra contar con metodologías analíticas validadas para el trabajo con muestras en fluidos biológicos. Se desarrolló un método por cromatografía líquida de alta resolución para la determinación de carbamazepina en plasma humano, se utilizó como fase móvil una mezcla de hidrógeno fosfato de sodio: acetonitrilo (65:35) ajustada a pH= 3,3 con ácido fosfórico, flujo de 1,2 mL/min y detección ultravioleta a 210 nm. Se empleó propilparabeno como estándar interno. Conforme con las regulaciones establecidas para la validación de métodos en fluidos biológicos se estudiaron los parámetros siguientes: estabilidad de las muestras, linealidad, especificidad, precisión, exactitud y límite de detección y cuantificación. El método resultó específico y sensible con un límite de detección y cuantificación de 0,9 y 1,0 ng, respectivamente. El método fue lineal, preciso y exacto en el rango de concentraciones de 1,07 a 12,67 µg/mL. La recuperación media no fue estadísticamente diferente del 100,0 por ciento. El analito en la matriz biológica propuesta permaneció en el periodo estudiado. La metodología descrita en este trabajo se aplica en nuestro caso al estudio que evalúa la biodisponibilidad y bioequivalencia de una formulación cubana de carbamazepina en voluntarios sanos(AU)