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1.
Reprod Biomed Online ; 35(6): 640-645, 2017 Dec.
Article in English | MEDLINE | ID: mdl-29111314

ABSTRACT

Perivitelline threads (PVT) are defined as thin filaments that extend across the perivitelline space connecting the zona pellucida with the oolemma or, in some cases, blastomere membrane. This is the first report of PVT in human embryos. Time-lapse imagery from 525 blastocysts with either tested ploidy, known implantation status, or both, were reviewed for the presence of PVT, the cell stage when PVT were first observed, association with fragmentation, ploidy or implantation potential; PVT were observed in most embryos (404/525 [77%]). The euploidy rate was similar in embryos with PVT (61/152 [40%]) and without PVT (17/35 [49%]). Implantation rates were also similar in embryos with PVT (64/259 [25%]) and without PVT (25/90 [28%]). In the embryos in which PVT were observed, 98% (396/404) developed at the two-cell stage. In most embryos (384/404 [95%]), PVT were observed to directly pull fragments from the embryo. Fragmentation occurred significantly less frequently in embryos without PVT compared with PVT (81/121 [67%] versus 388/404 [96%]; P < 0.001). These data suggest an association between PVT and fragmentation. This study is limited in that PVT were not characterized so their nature and origin remain unknown and to be determined in future studies.


Subject(s)
Embryo, Mammalian/cytology , Cleavage Stage, Ovum , Embryo Implantation , Humans , Microscopy , Ploidies , Retrospective Studies , Time-Lapse Imaging
2.
Hum Fertil (Camb) ; 19(3): 199-206, 2016 Sep.
Article in English | MEDLINE | ID: mdl-27662416

ABSTRACT

A retrospective, cohort study of high-risk patients undergoing IVF treatment was performed to assess if there is a difference in clinical pregnancy rate, live birth rate and the incidence of ovarian hyperstimulation syndrome, when a GnRH agonist (GnRHa) trigger with intensive luteal support is compared to human chorionic gonadotropin (hCG) with standard luteal support. The control group consisted of 382 high-risk patients having a GnRH antagonist protocol with 194 receiving an hCG trigger. All patients had ≥18 follicles ≥11mm or serum oestradiol >18,000pmol/l on the day of trigger. Patients had a single or double embryo transfer at cleavage or blastocyst stage. Logistic regression was used to adjust for differences between the groups. An intention-to-treat analysis of all cycles was performed. No statistically significant differences were observed in terms of positive pregnancy test, clinical pregnancy rate and live birth rate. Only one patient (0.3%) was hospitalized with severe OHSS in the GnRHa group, compared to 26 patients (13%) in the hCG group. In conclusion, GnRHa trigger is associated with similar pregnancy rates with hCG trigger and a significant reduction in hospitalization for severe OHSS after an intention to treat analysis was performed.


Subject(s)
Chorionic Gonadotropin/adverse effects , Fertility Agents, Female/adverse effects , Follicle Stimulating Hormone/adverse effects , Gonadotropin-Releasing Hormone/analogs & derivatives , Infertility, Female/therapy , Ovarian Hyperstimulation Syndrome/epidemiology , Ovulation Induction/adverse effects , Adult , Female , Fertility Agents, Female/therapeutic use , Fertilization in Vitro/adverse effects , Follicle Stimulating Hormone/therapeutic use , Gonadotropin-Releasing Hormone/adverse effects , Gonadotropin-Releasing Hormone/antagonists & inhibitors , Gonadotropin-Releasing Hormone/therapeutic use , Hormone Antagonists/adverse effects , Hormone Antagonists/therapeutic use , Humans , Incidence , Luteal Phase , Ovarian Hyperstimulation Syndrome/etiology , Ovulation Induction/methods , Pregnancy , Pregnancy Outcome , Pregnancy Rate , Retrospective Studies
3.
Hum Reprod ; 31(7): 1501-7, 2016 07.
Article in English | MEDLINE | ID: mdl-27112701

ABSTRACT

Cryopreservation of oocytes has been proposed as a way of storing gametes in young patients at high risk of infertility and premature ovarian failure. Recent advances in cryobiology have yielded promising results, leading to oocyte cryopreservation becoming a mainstay of fertility preservation. In this case series, we describe the feasibility of performing ovarian stimulation, and the ethical challenges faced, in teenage girls, aged 14-18 years, prior to undergoing bone marrow transplant for sickle cell anaemia. All eight consecutive cases completed ovarian stimulation and oocyte retrieval with mature oocytes being found and cryopreserved for each patient. The mean dose of gonadotrophin stimulation was 2134.38 IU (95% CI 1593.34-2675.4) and the mean duration of treatment was 11 days (95% CI 10.02-11.98). The mean number of oocytes retrieved was 14.88 (95% CI 7.39-22.36), of which a mean of 12.13 (95% CI 4.72-19.54) oocytes were mature and cryopreserved. There was one case of moderate ovarian hyperstimulation syndrome that required hospital admission for supportive treatment. Oocyte cryopreservation is a technique that can be successfully employed after the retrieval of mature oocytes from the peripubertal ovary, restoring hope to these patients, and their families, of having their own genetic children in the future.


Subject(s)
Fertility Preservation/ethics , Oocyte Retrieval/ethics , Adolescent , Age Factors , Bone Marrow Transplantation , Cryopreservation , Female , Humans , Oocyte Retrieval/methods , Oocytes/growth & development , Ovulation Induction/methods
4.
J Clin Invest ; 124(8): 3667-77, 2014 Aug.
Article in English | MEDLINE | ID: mdl-25036713

ABSTRACT

BACKGROUND: Patients with mutations that inactivate kisspeptin signaling are infertile. Kisspeptin-54, the major circulating isoform of kisspeptin in humans, potently stimulates reproductive hormone secretion in humans. Animal studies suggest that kisspeptin is involved in generation of the luteinizing hormone surge, which is required for ovulation; therefore, we hypothesized that kisspeptin-54 could be used to trigger egg maturation in women undergoing in vitro fertilization therapy. METHODS: Following superovulation with recombinant follicle-stimulating hormone and administration of gonadotropin-releasing hormone antagonist to prevent premature ovulation, 53 women were administered a single subcutaneous injection of kisspeptin-54 (1.6 nmol/kg, n = 2; 3.2 nmol/kg, n = 3; 6.4 nmol/kg, n = 24; 12.8 nmol/kg, n = 24) to induce a luteinizing hormone surge and egg maturation. Eggs were retrieved transvaginally 36 hours after kisspeptin injection, assessed for maturation (primary outcome), and fertilized by intracytoplasmic sperm injection with subsequent transfer of one or two embryos. RESULTS: Egg maturation was observed in response to each tested dose of kisspeptin-54, and the mean number of mature eggs per patient generally increased in a dose-dependent manner. Fertilization of eggs and transfer of embryos to the uterus occurred in 92% (49/53) of kisspeptin-54-treated patients. Biochemical and clinical pregnancy rates were 40% (21/53) and 23% (12/53), respectively. CONCLUSION: This study demonstrates that a single injection of kisspeptin-54 can induce egg maturation in women with subfertility undergoing in vitro fertilization therapy. Subsequent fertilization of eggs matured following kisspeptin-54 administration and transfer of resulting embryos can lead to successful human pregnancy. TRIAL REGISTRATION: ClinicalTrials.gov NCT01667406.


Subject(s)
Fertilization in Vitro/methods , Kisspeptins/administration & dosage , Oocytes/drug effects , Oocytes/growth & development , Adult , Dose-Response Relationship, Drug , Female , Fertility Agents, Female/administration & dosage , Fertilization in Vitro/adverse effects , Follicle Stimulating Hormone/administration & dosage , Gonadotropin-Releasing Hormone/administration & dosage , Gonadotropin-Releasing Hormone/analogs & derivatives , Humans , Infertility/physiopathology , Infertility/therapy , Kisspeptins/adverse effects , Kisspeptins/physiology , Ovulation/drug effects , Pregnancy , Pregnancy, Ectopic/etiology , Recombinant Proteins/administration & dosage , Recombinant Proteins/adverse effects
5.
Curr Opin Oncol ; 26(5): 501-7, 2014 Sep.
Article in English | MEDLINE | ID: mdl-25046204

ABSTRACT

PURPOSE OF REVIEW: To review currently available options in fertility preservation in cancer patients, report on emerging techniques, and highlight the importance of time sensitivity and recording of outcomes. RECENT FINDINGS: Fertility preservation in cancer patients is a rapidly expanding area of medicine. Recent success with experimental techniques such as oocyte cryopreservation and ovarian tissue cryopreservation exemplify the need for follow-up data collection. SUMMARY: Results of fertility outcomes in cancer patients should form an integral and important part of the pretreatment counselling process for cancer patients but limited published data from larger cohorts exist. The formation of a growing fertility preservation database would, therefore, allow ease of data analysis and more robust results.


Subject(s)
Cryopreservation , Fertility Preservation/methods , Neoplasms/pathology , Oocytes , Ovary , Female , Humans
6.
J Assist Reprod Genet ; 30(6): 761-4, 2013 Jun.
Article in English | MEDLINE | ID: mdl-23640373

ABSTRACT

PURPOSE: Partial molar pregnancies are rare conceptions characterized by having 69 rather than 46 chromosomes, the additional chromosome complement usually occurring as a result of fertilization of the ovum by two sperm. Although assisted conception with intracytoplasmic sperm injection (ICSI) should prevent the development of a partial molar pregnancy, occasional cases have been described after assisted conception using ICSI. The objective of this study was to investigate the cause of partial molar pregnancy in a couple who had undertaken assisted conception with ICSI. METHODS: Fluorescent microsatellite genotyping of DNA from the couple and tissue from their partial molar pregnancy was performed in order to confirm diagnosis and investigate the origin of the additional chromosome set. RESULTS: Genotyping confirmed that the partial molar tissue was triploid with an additional chromosome complement from the father. Genotyping of additional loci proximal to the centromere demonstrated that the two paternal sets of chromosomes originated in a single sperm with a double complement of paternal DNA resulting from non-reduction at the second meiotic division. CONCLUSIONS: This study confirms that partial molar pregnancy may occur after assisted conception with ICSI and that this occurs as a result of fertilization with a diploid sperm.


Subject(s)
Fertilization in Vitro , Hydatidiform Mole/genetics , Sperm Injections, Intracytoplasmic , Spermatozoa/pathology , Adult , Diploidy , Female , Genotyping Techniques , Humans , Hydatidiform Mole/pathology , Male , Microsatellite Repeats/genetics , Pregnancy
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