ABSTRACT
The ultimate stadium larva of Gynacantha cylindrata Karsch, 1891 is described and illustrated based on fifteen male and female exuviae from Bundibugyo, Uganda. The larva resembles those of the few described African species of the genus but shows a denser coverage of spine-like setae on body surface and abundance of hair-like setae on mouthparts. The female gonapophyses appear to be the longest described in the genus up to now and similar to those of G. villosa, a species included in the same group of African species.
Subject(s)
Larva/growth & development , Odonata/anatomy & histology , Animal Structures/anatomy & histology , Animal Structures/growth & development , Animals , Body Size , Female , Larva/anatomy & histology , Larva/classification , Male , Odonata/classification , Odonata/growth & development , Organ Size , UgandaABSTRACT
We developed a molecular assay to detect predation on Anopheles gambiae sensu lato (s.l.) mosquitoes. This intergenic spacer ribosomal DNA polymerase chain reaction assay and restriction enzyme analysis uses An. gambiae-specific primers to detect mosquito DNA in the DNA extracts from whole invertebrate predators, which enables identification of species (An. gambiae s.s. versus An. arabiensis) and molecular forms (M versus S in An. gambiae s.s.). We show that An. gambiae s.l. DNA can be detected after ingestion by members of the families Lestidae (order Odonata) after four hours, Libellulidae (order Odonata) after six hours, and Notonectidae (order Hemiptera) after 24 hours. This method is an improvement over previously published methods because of ease of execution and increased time of detection after ingestion.
