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1.
Hybridoma ; 16(2): 175-82, 1997 Apr.
Article in English | MEDLINE | ID: mdl-9145320

ABSTRACT

A major product of osteoclasts, tartrate-resistant acid phosphatase (TRAP) is an essential but insufficient enzyme for bone resorption. TRAP is an excellent cell marker for osteoclasts and macrophages and is being investigated as a serum marker for osteoclast activity in diseases of bone destruction. For decades, TRAP has also been used as a marker for hairy cell leukemia. Immunoassays for TRAP are sought to increase the sensitivity and specificity of the TRAP test for bone and hairy cells. Our laboratory recently developed a monoclonal antibody to TRAP (9C5) useful for immunohistochemical identification of TRAP-positive cells in paraffin sections. Herein, we characterize 9C5 in greater detail and report production of another anti-TRAP monoclonal antibody antibody (14G6) reactive with native, active enzyme antigen. Enzyme immunoassay, immunoprecipitation, western blot, and immunohistochemical analyses revealed the contrasting properties of 9C5 and 14G6. Antibody 9C5 reacts with a heat-denatured epitope and is suitable for denaturing western blot analysis and for immunohistochemistry. Antibody 14G6 reacts with a conformational determinant destroyed by heat and is suitable for immunoprecipitation of active TRAP, although 20% to 30% of activity is inhibited in the immune complexes. Having characterized several properties of these anti-TRAP antibodies, 9C5 and 14G6 may be useful for development of TRAP-specific immunoassays in bone pathology and hematology. They will certainly be of use for the study of biosynthesis, regulation, expression, and function of TRAP.


Subject(s)
Acid Phosphatase/immunology , Antibody Specificity , Immunohistochemistry/methods , Isoenzymes/immunology , Antibodies, Monoclonal , Biomarkers , Blotting, Western , Bone Resorption/enzymology , Bone Resorption/immunology , Epitopes , Humans , Hybridomas , Immunoenzyme Techniques , Leukemia, Hairy Cell/enzymology , Leukemia, Hairy Cell/immunology , Macrophages/immunology , Monocytes/immunology , Tartrate-Resistant Acid Phosphatase
2.
South Med J ; 88(12): 1260-3, 1995 Dec.
Article in English | MEDLINE | ID: mdl-7502121

ABSTRACT

Splenic lymphoma with villous lymphocytes (SLVL) is a recently described low-grade non-Hodgkin's lymphoma that may be confused with chronic lymphocytic leukemia and hairy cell leukemia. Herein we report a case of SLVL and show how clinical features, cellular morphology, and flow cytometric analysis are used to distinguish among these three disorders. A newly developed immunocytochemical technique that allows detection of tartrate-resistant acid phosphatase on formalin-fixed, paraffin-embedded specimens was useful in refining our diagnosis of SLVL. Establishing the precise diagnosis is important because treatment is different for each of the three chronic lymphoproliferative malignancies.


Subject(s)
Lymphocytes/pathology , Lymphoma, Non-Hodgkin/diagnosis , Aged , Flow Cytometry , Humans , Immunohistochemistry , Lymphoma, Non-Hodgkin/pathology , Male
3.
Blood ; 85(10): 2839-44, 1995 May 15.
Article in English | MEDLINE | ID: mdl-7742545

ABSTRACT

Tartrate-resistant acid phosphatase (TRAcP) has been an indispensible marker for hairy cell leukemia (HCL) for over two decades. However, the traditional TRAcP cytochemical stain cannot be performed effectively on sections of paraffin-embedded tissues that are important resources for histopathologic evaluation in diagnosis and treatment of HCL. Wide variation in expression of TRAcP activity by hairy cells (HCs) within and among patients is an interesting biologic phenomenon that has not been explained and can cause some diagnostic uncertainty as well. To solve the problem of staining TRAcP in paraffin sections and to begin to address the questions of variable TRAcP expression in HCL, we developed a monoclonal antibody to TRAcP, 9C5, for immunohistochemical identification of HCs. In smears of blood and bone marrow, immunocytochemistry of TRAcP using 9C5 was as specific but slightly less sensitive than direct cytochemical staining of enzymatic activity. In paraffin sections of spleen and bone marrow from HCL patients, immunohistochemistry with 9C5 stained the HCs with high sensitivity and specificity and clearly showed the characteristic diffuse infiltration by HCs. Other cells noted to stain strongly with 9C5 were occasional macrophages in bone marrow smears and osteoclasts and occasional tissue macrophages in paraffin sections. These are cells known to express abundant TRAcP activity. Immunohistochemistry with anti-TRAcP monoclonal antibody 9C5 may have utility as an added option in the diagnosis of HCL, as a means to evaluate residual disease in HCL patients undergoing new treatments, and as a way to address questions regarding variable expression of TRAcP activity by HCs within and among patients with HCL. Also, 9C5 has potential as a reagent for the immunoassay of bone-derived serum TRAcP in patients with certain bone diseases and cancers with bone metastasis.


Subject(s)
Acid Phosphatase/analysis , Isoenzymes/analysis , Leukemia, Hairy Cell/diagnosis , Acid Phosphatase/immunology , Antibodies, Monoclonal/immunology , Biomarkers, Tumor , Humans , Immunohistochemistry/methods , Leukemia, Hairy Cell/enzymology , Tartrate-Resistant Acid Phosphatase
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