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1.
Musculoskelet Surg ; 106(1): 83-87, 2022 Mar.
Article in English | MEDLINE | ID: mdl-32949004

ABSTRACT

PURPOSE: Periprosthetic fractures after hip prosthesis represent a constantly increasing clinical problem and a challenging complication to treat surgically. Among these, type B proximal femur fractures should be diagnosed correctly to be treated surgically. The aim of this study was to re-evaluate the type of surgical treatment of periprosthetic fractures. METHODS: We examined the cases treated between January 2012 and February 2018, classifying them according to the U.C.S. AO/OTA. We evaluated the radiographic outcome according to the Beals and Tower criteria. Patients still alive were also re-evaluated according to the H.H.S. and the WOMAC score. RESULTS: We treated 48 patients (12 men, 35 women, average age 81 years), divided into 24 type B1, 14 type B2 and 10 type B3 fractures. The overall consolidation rate was 95.4%, while the major complication (implant dislocation, pseudoarthrosis and deep infection) rate was 12.5%. Clinically, it was possible to reassess 34 patients with a mean follow-up of 38.4 months, an average HHS of 75.89 and a mean WOMAC score of 79.93. CONCLUSIONS: Periprosthetic type B fractures are difficult to manage and require careful preoperative planning and appropriate intraoperative management. However, the overall clinical and radiographic result was satisfactory, although patients should still be aware of the risk of complications associated with this type of fracture.


Subject(s)
Arthroplasty, Replacement, Hip , Femoral Fractures , Periprosthetic Fractures , Aged, 80 and over , Arthroplasty, Replacement, Hip/adverse effects , Female , Femoral Fractures/diagnostic imaging , Femoral Fractures/etiology , Femoral Fractures/surgery , Femur/surgery , Fracture Fixation, Internal , Humans , Male , Periprosthetic Fractures/diagnostic imaging , Periprosthetic Fractures/etiology , Periprosthetic Fractures/surgery , Reoperation , Retrospective Studies , Treatment Outcome
3.
Contraception ; 63(6): 303-7, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11672551

ABSTRACT

The effects of a 24-day regimen containing 15 microg ethinyl estradiol (EE) plus 60 microg gestodene on cycle control and on hemostasis, were evaluated in 58 healthy women (age 19-47 years). All women received the pill for 12 months. Withdrawal bleeding at every cycle during the tablet-free interval was experienced by 84.5% of the women. The overall incidence of irregular bleedings was 19.3%. Hemostasis was evaluated in 20 women. No changes in plasma fibrinogen concentrations, nor in prothrombin fragment F1+2 were observed. A slight increase in thrombin-antithrombin III complexes was observed after 6 and 12 months of oral contraceptive use. Antithrombin III activity significantly increased after one-year of pill intake. The concentrations of tissue plasminogen activator and plasminogen activator inhibitor, both antigen and activity, did not change. These results show that very low doses of EE, such as 15 microg, do not impair hemostasis in healthy females. However, the reduction for the EE dose is responsible of some of the effects on cycle control.


Subject(s)
Contraceptives, Oral, Synthetic/administration & dosage , Contraceptives, Oral, Synthetic/adverse effects , Ethinyl Estradiol/administration & dosage , Ethinyl Estradiol/adverse effects , Hemostasis/drug effects , Hemostasis/physiology , Menstrual Cycle/drug effects , Menstrual Cycle/physiology , Norpregnenes/administration & dosage , Norpregnenes/adverse effects , Adolescent , Adult , Analysis of Variance , Enzyme-Linked Immunosorbent Assay , Female , Humans , Time Factors , Whole Blood Coagulation Time
5.
Eur J Contracept Reprod Health Care ; 4(3): 113-8, 1999 Sep.
Article in English | MEDLINE | ID: mdl-10574636

ABSTRACT

The concurret use of smoking and oral contraceptives affects the hemostatic balance, thereby inducing a thrombophilic state. In order to clarify the effects of this association on the hemostatic system, the possible changes in the markers of activation of coagulation (thrombin-antithrombin III complexes and prothrombin fragment F1+2) were evaluated in 35 women given a third-generation oral contraceptive for 6 months; 13 of these women (37.1%) were mild or moderate smokers. No differences were found in basal levels of the coagulation and fibrinolytic parameters between smokers and non-smokers. During oral contraceptive administration, both F1+2 fragment and thrombin-antithrombin III complex concentrations significantly increased both in smokers and in non-smokers (p < 0.01). Fibrinogen plasma levels increased in both groups (p < 0.01). Antithrombin III activity was reduced in both groups during treatment, but the difference was significant only in smokers (p < 0.05). Although the sample size of smokers was too small to draw definitive conclusions, present results appeared to confirm previous data about the effect of the concurrent use of smoking and oral contraceptives on antithrombin III levels, but did not demonstrate any additional effect of moderate smoking on the activation of the clotting system induced by this oral contraceptive preparation.


Subject(s)
Antithrombin III/analysis , Blood Coagulation/drug effects , Desogestrel/administration & dosage , Ethinyl Estradiol/administration & dosage , Fibrinogen/analysis , Prothrombin/analysis , Smoking/adverse effects , Adolescent , Adult , Analysis of Variance , Blood Coagulation/physiology , Contraceptives, Oral, Combined/administration & dosage , Female , Humans , Reference Values , Sensitivity and Specificity
6.
Am J Med ; 107(4): 344-50, 1999 Oct.
Article in English | MEDLINE | ID: mdl-10527036

ABSTRACT

PURPOSE: Plasma levels of plasminogen activator inhibitor-1 are increased in obesity, hypertension, and diabetes. Their correlation with insulin levels supports the hypothesis that hypofibrinolysis may affect the development of atherosclerotic complications in patients with insulin resistance. To investigate the effect of insulin on fibrinolysis, we evaluated levels of plasminogen activator inhibitor-1 (PAI-1) and tissue plasminogen activator (tPA) antigens during insulin infusion in the forearm vascular beds of 8 healthy subjects. MATERIALS AND METHODS: Insulin was infused in the brachial artery of each subject to raise local venous concentrations to approximately 100 microU/mL. Blood samples were obtained from the brachial artery and vein at baseline, after 30, 60, 90, and 120 minutes of infusion, and 30 minutes after the end of the infusion. RESULTS: Following intra-arterial infusion of insulin, forearm blood flow (mean +/- SD) increased progressively from 2.7 +/- 0.6 to 4.0 +/- 0.6 mL/dL/min (P <0.01) and did not return to baseline after the end of the infusion. Plasminogen activator inhibitor-1 balance increased (345 +/- 160 versus 8 +/- 152 fmol/dL/min, P <0.02) at 60 minutes, reaching baseline levels after the end of the infusion. After 90 minutes, tPA balance increased (40 +/- 26 versus 7 +/- 29 fmol/dL/min, P <0.01) with a profile similar to forearm blood flow. CONCLUSIONS: Local hyperinsulinemia induces regional vasodilation and expression of PAI-1 and tPA antigens. An alteration of this physiological process could be involved in the development of hypofibrinolysis and atherosclerosis in states of insulin resistance.


Subject(s)
Hypoglycemic Agents/pharmacology , Insulin/pharmacology , Insulin/physiology , Plasminogen Activator Inhibitor 1/blood , Tissue Plasminogen Activator/blood , Adult , Blood Glucose/metabolism , Brachial Artery , Forearm/blood supply , Gene Expression Regulation/drug effects , Humans , Hypoglycemic Agents/administration & dosage , Hypoglycemic Agents/blood , Infusions, Intra-Arterial , Insulin/administration & dosage , Insulin/blood , Male , Reference Values , Regional Blood Flow , Time Factors
7.
Angiology ; 50(4): 273-82, 1999 Apr.
Article in English | MEDLINE | ID: mdl-10225462

ABSTRACT

Muscarinic agents release tissue plasminogen activator (t-PA) in the forearm circulation of normal subjects, but no information exists about their effect in those hypertensive patients in whom the response to endothelial-mediated vasodilators is blunted. Acetylcholine, an endothelium-dependent vasodilator and a muscarinic agonist that releases t-PA from in-vitro systems, and sodium nitroprusside, an endothelium-independent vasodilator, were infused into the brachial artery at rates calculated to cause a similar degree of vasodilatation. The study was performed in five elderly, smoking hypertensive patients in whom the clustering of detrimental factors for endothelial function permitted prediction of defective endothelial-mediated vasorelaxation, and five young, normotensive, nonsmoking male volunteers. Forearm blood flow was assessed by venous plethysmography; t-PA and plasminogen activator inhibitor 1 (PAI-1) antigen values were expressed as flow-dependent (net release, the product of venoarterial concentration gradient and forearm blood flow) or independent (absolute and fractional concentration gradients) indices. In patients, acetylcholine did not change flow and net release and concentration gradients of t-PA, suggesting that vasodilatation as such, possibly by increasing fluid shear stress, may induce t-PA release in human forearm. In normal subjects, acetylcholine and sodium nitroprusside increased t-PA antigen net release at the highest infusion rate, an effect attributable to forearm hyperperfusion, since absolute and fractional gradients did not change significantly. PAI-1 antigen did not change during either infusion in both controls and patients, indicating the absence of an endothelial pool to be mobilized acutely.


Subject(s)
Acetylcholine/pharmacology , Hypertension/physiopathology , Tissue Plasminogen Activator/blood , Vasodilation/drug effects , Vasodilator Agents/pharmacology , Adult , Aged , Analysis of Variance , Forearm/blood supply , Forearm/physiopathology , Humans , Hypertension/blood , Male , Nitroprusside/pharmacology , Plasminogen Activator Inhibitor 1/blood , Reference Values , Regional Blood Flow/drug effects , Time Factors , Tissue Plasminogen Activator/drug effects
8.
Arterioscler Thromb Vasc Biol ; 17(11): 2646-54, 1997 Nov.
Article in English | MEDLINE | ID: mdl-9409238

ABSTRACT

Vascular cell adhesion molecule-1 (VCAM-1) is a protein expressed on the surface of activated endothelial cells and expressed in early atherosclerosis. Because part of the protein is shed in the circulation and can be detected in peripheral plasma [soluble (s) VCAM-1], we hypothesized that sVCAM-1 may be a circulating marker of the presence and severity of atherosclerosis in humans. We selected 11 patients with essential hypertension plus peripheral vascular disease (PVD) and matched them for age, gender, body mass index, and smoking habits with 11 patients with uncomplicated essential hypertension (UH) and 11 healthy controls. We evaluated plasma concentrations of sVCAM-1 along with those of the soluble form of two other endothelial leukocyte adhesion molecules [sE-selectin and s-intercellular adhesion molecule-1 (sICAM-1)] and other markers of endothelial dysfunction/ damage [s-thrombomodulin, plasminogen activator inhibitor type I, and von Willebrand factor (vWF)]. We also measured insulin, glucose, fibrinogen, total and HDL cholesterol, and the urinary albumin excretion (UAE), which may also be related to atherosclerosis. Results of these assays were related to the echographic assessment of the maximum intima-media thickness (IMTmax) at the carotid bifurcation, as an index of atherosclerosis in the carotids. PVD patients had a clearly elevated IMTmax [2.7 (1.1-3.1) mm, median (range)] compared with both UH patients [1.2 (0.8-2.4) mm] and controls [1 (0.6-2) mm]. sVCAM-1 was clearly higher in PVD patients [990 (273-1808) ng/mL, median (range)] versus 340 (236-975) ng/mL in UH and 386 (204-835) ng/mL in controls, and it separated clinical categories better than sICAM-1, vWF, glucose, insulin, UAE, triglycerides, or total, LDL or HDL cholesterol, sVCAM-1 was also the best biohumoral correlate of IMTmax (R = .59; P < .001) in univariate analysis. Because many of the biohumoral variables assessed were mutually intercorrelated, they were entered in a multivariate analysis to assess their contribution in explaining IMTmax variability. sVCAM-1 remained the only independent predictor of IMTmax and totally abolished the contribution of other variables to IMTmax variability. Thus, sVCAM-1 is a good biohumoral correlate of overt atherosclerosis, independent of underlying hypertension, and may be an in vivo marker of endothelial activation. Its potential value as a surrogate for global risk assessment and its behavior in intervention studies remain to be determined.


Subject(s)
Arteriosclerosis/blood , Hypertension/blood , Peripheral Vascular Diseases/blood , Vascular Cell Adhesion Molecule-1/blood , Aged , Arteriosclerosis/complications , Arteriosclerosis/diagnostic imaging , Arteriosclerosis/epidemiology , Arteriosclerosis/pathology , Biomarkers , Blood Glucose/analysis , Carotid Stenosis/diagnostic imaging , Carotid Stenosis/etiology , Carotid Stenosis/pathology , E-Selectin/blood , Endothelium, Vascular/metabolism , Female , Humans , Hypertension/complications , Insulin/blood , Intercellular Adhesion Molecule-1/blood , Lipids/blood , Male , Middle Aged , Multivariate Analysis , Peripheral Vascular Diseases/complications , Plasminogen Activator Inhibitor 1/blood , Regression Analysis , Risk Factors , Sensitivity and Specificity , Single-Blind Method , Solubility , Ultrasonography , Vascular Cell Adhesion Molecule-1/chemistry , von Willebrand Factor/analysis
11.
Haemostasis ; 26(1): 16-22, 1996.
Article in English | MEDLINE | ID: mdl-8698274

ABSTRACT

Plasma levels of selected coagulation and fibrinolytic parameters (activated partial thromboplastin time, prothrombin time, fibrinogen, antithrombin III, protein C, thrombin-anti-thrombin III complexes (TAT), plasminogen activator inhibitor-1 (PAI-1), plasminogen, alpha 2-plasmin inhibitor) were evaluated in 90 patients with clinical suspicion of pulmonary embolism (PE). Plasma levels of fibrinogen, PAI-1 and TAT were significantly higher in patients than in controls (p < 0.01): evaluation of TAT displayed a sensitivity of 96.1% and specificity of 30.8%, and positive and negative predictive values of 64.5 and 85.7%, respectively. The number of nonperfused lung segments correlated directly with TAT levels (p < 0.01) and inversely with arterial pO2 values (p < 0.01). No significant difference was found in the other parameters between patients and controls. Our results suggest that the finding of normal TAT plasma levels can help to exclude PE in patients with clinically suspected PE.


Subject(s)
Antithrombin III , Peptide Hydrolases , Pulmonary Embolism/diagnosis , Aged , Aged, 80 and over , Blood Coagulation Tests , Evaluation Studies as Topic , Female , Fibrinolysis/physiology , Humans , Male , Middle Aged , Predictive Value of Tests , Pulmonary Embolism/blood
12.
Semin Thromb Hemost ; 22(6): 479-88, 1996.
Article in English | MEDLINE | ID: mdl-9122712

ABSTRACT

The physiological and pathophysiological state of tissues determines the exudation of plasma proteins, hemostasis, and fibrinolysis, i.e., inflammation, injury, and malignancy. The physiological controls of extravascular fibrinolysis ultimately rest on a balance between generation of the fibrinolytic enzyme(s), i.e., plasmin, elastase, cathepsins, etc., and inhibitors of the fibrinolytic enzyme(s), i.e., plasminogen activator inhibitors, alpha-2 plasmin inhibitor, alpha 1-protease inhibitors, etc. Moreover, it is the structural modification of fibrin that determines its stability toward proteolytic enzymes and physical duress. The structural modification of fibrin involves factor XIIIa-mediated cross-linking of interfibrin chains and alpha 2-plasmin inhibitor to fibrin. In turn, there are a number of agents that influence factor XIIIa catalytic activity (e.g., sulfhydryl agents, albumin, erythrocytes). The two key proenzymes, factor XIII and plasminogen, are tightly bound with the circulating fibrinogen molecules. Such high selective affinity for fibrin(ogen) provides the reaction specificity in a complex tissue fluid milieu and governs the kinetics of fibrinolysis. Any agents that interfere with such binding reactions, e.g., autoantibodies, may also affect the fibrinolytic reactions. Understanding these unique biochemical controls of factors involved in fibrinolysis may provide an insight into the complex regulatory process of extravascular fibrinolysis.


Subject(s)
Fibrinolysis/physiology , Animals , Factor XIII/metabolism , Factor XIII/physiology , Fibrin/metabolism , Fibrin/physiology , Humans , Plasminogen/metabolism , Plasminogen/physiology , Serum Albumin/metabolism , Shwartzman Phenomenon/physiopathology
13.
Semin Thromb Hemost ; 22(6): 489-96, 1996.
Article in English | MEDLINE | ID: mdl-9122713

ABSTRACT

The patterns of degradation and the influence of factor XIII polymerization on fibrin stability were examined in vitro following incubation with leukocyte elastase. In vivo experiments, various factor XIII-polymerized fibrin clots were implanted subcutaneously in mice to evaluate the stability of clots in the extravascular space. Both in vitro and in vivo lysis proceeded faster with nonpolymerized fibrin and was not influenced by the presence of cross-linked alpha 2-plasmin inhibitor. In vivo lysis of implanted clots was prevented by elastatinal, powerful elastase inhibitor, suggesting that granulocyte elastase is chiefly responsible for clot lysis in the extravascular space. To further extend investigations on the mechanisms of fibrinolysis in tissues, we evaluated fibrin and its degradation products in the synovial space. Expression of factor XIII in synovial cells and activities of coagulation factors, fibrinolytic enzymes, and inhibitors were investigated in the synovial fluid of rheumatoid arthritis patients. Immunohistochemical analysis showed deposits of insoluble fibrin on synovial membranes and pannus to an extent related to the progression of the disease. Factor XIII was expressed by fibroblasts and macrophages in the early stages of the disease, whereas in advanced stages factor XIII staining was associated with fibrin. The reduction of certain coagulation factors and high level of thrombin-antithrombin complexes in synovial fluid show a steady activation of the coagulation cascade. The evaluation of fibrinogen degradation products and the pattern of degradation of synovial fibrin(ogen) suggest the participation of leukocyte elastase in fibrin(ogen) lysis in synovial tissue of rheumatoid arthritis.


Subject(s)
Arthritis, Rheumatoid/metabolism , Arthritis, Rheumatoid/physiopathology , Fibrin/metabolism , Fibrinolysis/physiology , Models, Cardiovascular , Synovial Fluid/metabolism , Humans , Synovial Fluid/chemistry
14.
J Mol Med (Berl) ; 73(2): 89-93, 1995 Feb.
Article in English | MEDLINE | ID: mdl-7627635

ABSTRACT

Patients with liver failure can present both thrombotic and hemorrhagic complications because of the deficiency in coagulation factors and inhibitors (protein C and S, antithrombin III) and impairment of fibrinolytic balance. Here we report the case of a 63-year-old man with liver cirrhosis, recurrent thrombosis, and features of low-grade consumption coagulopathy, showing severe antithrombin III deficiency (about 30% of normal values). Treatment with antithrombin III (2000 U/day) and low doses of heparin (5000 U b.i.d.) was successful in modulating the coagulation system toward an antithrombotic effect. After discharge from hospital the ambulatory treatment with antithrombin III concentrates (2000 U twice a week) allowed the attainment of antithrombin III activity of about 60% and prevented the patient from recurrence of venous thrombosis.


Subject(s)
Antithrombin III/therapeutic use , Disseminated Intravascular Coagulation/complications , Liver Cirrhosis/complications , Thrombophlebitis/complications , Antithrombin III Deficiency , Disseminated Intravascular Coagulation/drug therapy , Female , Hemostasis/drug effects , Heparin/therapeutic use , Humans , Male , Middle Aged , Thrombophlebitis/drug therapy
15.
Hypertension ; 24(1): 24-9, 1994 Jul.
Article in English | MEDLINE | ID: mdl-8021004

ABSTRACT

In response to hypertension, arterioles remodel their structure, the heart develops myocardial hypertrophy, and the kidney reduces creatinine clearance and increases albuminuria. To better understand the interrelations among the target organs involved in hypertension, we evaluated minimal forearm vascular resistances--a hemodynamic index of arteriolar structure derived from mean blood pressure and maximal postischemic forearm blood flow--the echocardiographic indexes of cardiac structure, and urinary albumin excretion and creatinine clearance in 29 male mild to moderate non-macroalbuminuric essential hypertensive patients on no drugs and 11 age- and sex-matched normotensive control subjects. Minimal forearm resistances were elevated in hypertensive patients and correlated with left ventricular mass, wall thickness, and mean arterial pressure. Patients with abnormal minimal forearm resistances (2 SD above normal) were characterized by higher pressure, greater wall thickness, lower creatinine clearance, and higher albumin excretion, suggesting that maximal forearm flow capacity does relate to the hemodynamic load exerted on both the kidney and heart. However, the correlation with cardiac structure and mean arterial pressure explained only part of the variability of minimal forearm resistances. Furthermore, no correlation among these parameters was found when hypertensive patients were evaluated separately from normotensive subjects, possibly because of heterogeneous factors active on arteriolar structure and unrelated to the pressor load. Overall, the data suggest that the development of abnormal minimal forearm resistances in the course of the hypertensive process is related to the pressor load, but its details need further understanding.


Subject(s)
Forearm/blood supply , Heart/physiopathology , Hypertension/physiopathology , Kidney/physiopathology , Adult , Aged , Albuminuria/etiology , Echocardiography , Humans , Male , Middle Aged , Regional Blood Flow
16.
Lancet ; 344(8914): 14-8, 1994 Jul 02.
Article in English | MEDLINE | ID: mdl-7912295

ABSTRACT

Microalbuminuria (urinary albumin excretion between 20 and 200 micrograms/min) and endothelial dysfunction coexist in patients with essential hypertension. To evaluate whether the two phenomena are related and the determinants of that association, we recruited 10 untreated males with essential hypertension and microalbuminuria without diabetes to be compared with an equal number of matched patients with essential hypertension excreting albumin in normal amounts and 10 normal controls. The status of endothelial function was inferred from circulating von Willebrand Factor antigen (vWF), a glycoprotein secreted in greater amounts when the vascular endothelium is damaged. vWF concentrations were higher in hypertensive patients with microalbuminuria than in hypertensive patients without and controls. Individual vWF and urine albumin-excretion values were correlated (r = 0.55, p < 0.002). Blood pressure correlated with both urinary albumin excretion and vWF. Left ventricular mass index and minimal forearm vascular resistances were comparable in patients with hypertension and higher than in controls; total and low-density lipoprotein cholesterol, triglycerides, lipoprotein-a, Factor VII, and plasminogen activator inhibitor-1 did not differ. Fibrinogen was higher and creatinine clearance lower in microalbuminurics. Albuminuria in essential hypertension may reflect systemic dysfunction of the vascular endothelium, a structure intimately involved in permeability, haemostasis, fibrinolysis, and blood pressure control. This abnormality may have important physiopathological implications and expose these patients to increased cardiovascular risk.


Subject(s)
Albuminuria , Endothelium, Vascular/physiopathology , Hypertension/urine , Hemodynamics , Humans , Hypertension/blood , Hypertension/physiopathology , Lipids/blood , Male , Middle Aged , von Willebrand Factor/analysis
17.
Respiration ; 61(2): 93-8, 1994.
Article in English | MEDLINE | ID: mdl-8008995

ABSTRACT

We studied 84 consecutive patients referred with the suspicion of pulmonary embolism (PE) to investigate the influence of clinical and hematological profiles on the diagnosis and severity of this disease and recovery. Diagnosis of PE was confirmed in 48 out of 84 patients by perfusion scintigraphy and/or pulmonary arteriography. Severity of PE and entity of recovery were investigated by measuring standard PaO2 on blood gas analysis and the number of unperfused lung segments ULS on perfusion scintigraphy. Most common clinical predisposing conditions were more frequent, though not significantly so, in embolic patients and a very low prevalence of PE was appreciable in patients without clear predisposing conditions. Among coagulation factors, only thrombin-antithrombin (TAT) complexes were twice as high in embolic as in nonembolic patients (14.0 +/- 13.6 vs. 7.0 +/- 4.2 ng/ml; p < 0.02), while there was no statistically significant difference between embolic and nonembolic patients for activated partial thromboplastin time, prothrombin time, antithrombin III, protein C, fibrinogen, plasminogen, alpha 2-plasmin inhibitor, and plasminogen activator inhibitor-1. Sensitivity and specificity of TAT complexes in diagnosis of PE were 95.8% and 30.5%, respectively. Therefore, normal values of TAT complexes may help exclude the diagnosis of PE, while abnormal values allow to reinforce the clinical suspicion of PE. No relation was found between coagulation parameters and the severity of PE. The follow-up of 48 patients with confirmed PE was favorable on the average; however, neither the presence of predisposing conditions nor abnormal coagulation parameters allow to predict the degree of functional and scintigraphic improvement during follow-up.


Subject(s)
Blood Coagulation , Pulmonary Embolism/diagnosis , Adult , Aged , Aged, 80 and over , Antithrombin III/analysis , Female , Humans , Lung/diagnostic imaging , Male , Middle Aged , Peptide Hydrolases/analysis , Pulmonary Embolism/blood , Pulmonary Embolism/diagnostic imaging , Radionuclide Imaging
18.
Int J Tissue React ; 16(2): 89-93, 1994.
Article in English | MEDLINE | ID: mdl-7960505

ABSTRACT

Selected coagulation and fibrinolytic factors were evaluated in plasma and synovial fluid (SF) of 10 rheumatoid arthritis (RA) patients. Increased levels of fibrinogen were observed in plasma (p < 0.01), but only a trace amount of structurally intact fibrinogen was detected in the SF of RA patients, while immunostaining showed deposits of insoluble fibrin in their synovial membranes. Reduced levels of protein C, antithrombin III and coagulation factors II, V, VII, VIII, IX, XII and XIII (p < 0.01), and high levels of thrombin-antithrombin III (TAT) complexes (p < 0.01), were found in SF as compared to their corresponding plasma levels. The increased levels of fibrinogen, TAT complexes, B beta 15-42 peptide and plasminogen activator inhibitor-1 (PAI-1) in plasma (p < 0.01) are consistent with an enhanced fibrin turnover and endothelial perturbation due to a systemic inflammatory state. Plasminogen and alpha 2-plasmin inhibitor activity in SF were significantly reduced as compared to the plasma levels (p < 0.01), whereas an increase in PAI-1 activity was found in SF as compared to plasma (p < 0.01). The detection of D-dimer and B beta 15-42 peptide (p < 0.01) in SF suggests an involvement of plasmin in the degradation of fibrin generated in synovial tissue. The high levels of elastase-alpha 1-proteinase inhibitor complexes and of thrombin-increasable fibrinopeptide A, as well as the pattern of fibrinogen degradation as identified in SF by double-dimension immunoelectrophoresis, suggest that elastase released from exudated granulocytes may play an important role in fibrino(geno)lysis and tissue damage in RA joints.


Subject(s)
Arthritis, Rheumatoid/blood , Fibrinolysin/physiology , Fibrinolysis/physiology , Pancreatic Elastase/physiology , Adult , Antithrombin III/analysis , Arthritis, Rheumatoid/enzymology , Arthritis, Rheumatoid/physiopathology , Blood Coagulation Factors/analysis , Female , Fibrin/analysis , Humans , Immunoelectrophoresis , Immunohistochemistry , Male , Plasminogen/analysis , Plasminogen Activator Inhibitor 1/blood , Protein C/analysis , Synovial Fluid/chemistry , Synovial Membrane/chemistry , Synovial Membrane/embryology
19.
Nephron ; 63(2): 226-9, 1993.
Article in English | MEDLINE | ID: mdl-8450917

ABSTRACT

We report a case of phosphate diabetes in a patient with the syndrome of inappropriate secretion of antidiuretic hormone (SIADH) associated with sarcoidosis. Our patient was affected by systemic sarcoidosis and he fits the criteria of Schwartz for the diagnosis of SIADH. He presented with phosphate diabetes which appeared during demeclocycline (DMC) therapy and persisted for about 1 month from the end of DMC. It constitutes the fourth case of phosphate diabetes induced by tetracycline described in the literature and it is the third case of SIADH associated with sarcoidosis.


Subject(s)
Demeclocycline/adverse effects , Hypophosphatemia, Familial/chemically induced , Inappropriate ADH Syndrome/complications , Sarcoidosis/complications , Humans , Inappropriate ADH Syndrome/diagnosis , Male , Middle Aged , Sarcoidosis/diagnosis
20.
Thromb Res ; 67(6): 643-54, 1992 Sep 15.
Article in English | MEDLINE | ID: mdl-1440530

ABSTRACT

Selected coagulation and fibrinolytic parameters were assessed in 40 insulin dependent diabetes mellitus patients with varying degrees of metabolic control; 30 healthy subjects matched for age and sex formed the control group. Activated Partial Thromboplastin Time, Prothrombin Time, Fibrinogen, Factor VII, Antithrombin III, Protein C, Plasminogen, alpha 2-Plasmin Inhibitor, Plasminogen Activator Inhibitor-1, tissue-Plasminogen Activator were functionally evaluated. Antigenic levels of tissue-Plasminogen Activator, Thrombin-Antithrombin complexes and fibrinolytic specific product B beta 15-42 were also determined. Compared to the control group diabetic patients displayed significantly higher levels of Fibrinogen (p < 0.01), Factor VII (p < 0.01), Thrombin-Antithrombin complexes (p < 0.01) and Plasminogen Activator Inhibitor-1 activity (p < 0.01). Regardless of the normal level of the tissue-Plasminogen Activator-related antigen, diabetic patients had tissue-Plasminogen Activator activity lower than the control group (p < 0.05). Coagulation Factor VII and Thrombin-Antithrombin complexes were increased only in the patients with poor metabolic control (p < 0.01). Activated Partial Thromboplastin Time, Prothrombin Time, Antithrombin III, Protein C, Plasminogen, alpha 2-Plasmin Inhibitor, B beta 15-42 fibrin peptide were found to be in the normal range. Fibrinogen correlated positively with fasting blood glucose (p < 0.05) and Thrombin-Antithrombin complexes with glycosylated haemoglobin (p < 0.05), whereas Factor VII was positively correlated with glycemia (p < 0.01) and glycosylated haemoglobin (p < 0.05). Higher levels of Fibrinogen were found in patients affected by nephropathy (p < 0.005) or neuropathy (p < 0.05). These results demonstrate an impairment of the haemostatic balance in diabetic patients, that is a possible hypercoagulable state, which represents an important factor in the pathogenesis of atherosclerotic complications.


Subject(s)
Blood Coagulation/physiology , Diabetes Mellitus, Type 1/blood , Fibrinolysis/physiology , Adult , Amino Acid Sequence , Antithrombin III/metabolism , Diabetic Angiopathies/etiology , Factor VII/metabolism , Female , Fibrinogen/metabolism , Humans , Male , Molecular Sequence Data , Oligopeptides/chemistry , Peptide Hydrolases/metabolism , Plasminogen Activator Inhibitor 1/blood , Plasminogen Activators/blood , Substrate Specificity
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